FastQCFastQC Report
Fri 10 Feb 2017
ERR1631513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281116
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36061.2827444898191493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30631.0895857937648514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26960.9590347045347829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.3813372415657593No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8770.31197085900482363No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA5500.19564877132571606No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.14904879124631826No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA3930.13979994023819348No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.12877246403619858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.12770528891987648No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA3570.12699383884232843No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA3430.12201368829949201No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC3420.121657963260718No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT3300.11738926279542963No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC3290.11703353775665562No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT2940.10458316139956458No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT2880.10244881116692041No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.10067018597305027No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAACC303.5926243E-430.83333426
CCCGTTG303.5926243E-430.83333431
TAACCCG303.5926243E-430.83333428
ACCCGTT303.5926243E-430.83333430
GTAACCC303.5926243E-430.83333427
CTCACGG250.00548937129.59999815
CCATGCT405.924422E-527.759
GAGACAC405.924422E-527.7514
AGACACC405.924422E-527.7515
GCGGGTA358.852666E-426.42857223
GATAGAG509.059229E-625.8999987
CCCTAAG451.319741E-424.6666661
TAAGATA451.319741E-424.6666664
CGGGTAA400.001927579923.12524
TATGAGT400.001927579923.1254
CCTTTCC400.001927579923.12537
AACGCCG400.001927579923.1255
TCATGGA400.001927579923.1252
TAGAGGA502.6950723E-422.1999999
GACACCT502.6950723E-422.19999916
Produced by FastQC (version 0.11.5)