##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631513.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	281116
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.96609940380483	34.0	33.0	34.0	31.0	34.0
2	33.1006844149746	34.0	33.0	34.0	31.0	34.0
3	33.15417123180467	34.0	33.0	34.0	31.0	34.0
4	36.414636662445396	37.0	37.0	37.0	35.0	37.0
5	36.42292505584883	37.0	37.0	37.0	35.0	37.0
6	36.50558844035914	37.0	37.0	37.0	35.0	37.0
7	36.51854750352168	37.0	37.0	37.0	35.0	37.0
8	36.531951222982684	37.0	37.0	37.0	35.0	37.0
9	38.36387825666273	39.0	39.0	39.0	37.0	39.0
10	38.321265954267986	39.0	39.0	39.0	37.0	39.0
11	38.379103288322256	39.0	39.0	39.0	37.0	39.0
12	38.354444428634444	39.0	39.0	39.0	37.0	39.0
13	38.381401272072736	39.0	39.0	39.0	37.0	39.0
14	39.85717995418261	41.0	40.0	41.0	38.0	41.0
15	39.82030905391369	41.0	40.0	41.0	38.0	41.0
16	39.75997097283684	41.0	40.0	41.0	38.0	41.0
17	39.76157173551132	41.0	40.0	41.0	38.0	41.0
18	39.81766245962521	41.0	40.0	41.0	38.0	41.0
19	39.86047041079127	41.0	40.0	41.0	38.0	41.0
20	39.82838045504347	41.0	40.0	41.0	38.0	41.0
21	39.82161456480599	41.0	40.0	41.0	38.0	41.0
22	39.759458728781	41.0	40.0	41.0	38.0	41.0
23	39.699771624525106	41.0	40.0	41.0	37.0	41.0
24	39.682789311174034	41.0	40.0	41.0	37.0	41.0
25	39.66236002219724	41.0	40.0	41.0	37.0	41.0
26	39.536419129469685	41.0	40.0	41.0	37.0	41.0
27	39.45705331606881	41.0	39.0	41.0	36.0	41.0
28	39.38282061497745	41.0	39.0	41.0	35.0	41.0
29	39.29741459041819	41.0	39.0	41.0	35.0	41.0
30	39.15041477539521	41.0	39.0	41.0	35.0	41.0
31	39.00202407547062	41.0	39.0	41.0	35.0	41.0
32	38.87576658745856	40.0	38.0	41.0	35.0	41.0
33	38.765107642396735	40.0	38.0	41.0	35.0	41.0
34	38.69914910570725	40.0	38.0	41.0	35.0	41.0
35	38.56756285661435	40.0	38.0	41.0	35.0	41.0
36	38.456711820031586	40.0	38.0	41.0	35.0	41.0
37	38.34977020162495	40.0	38.0	41.0	35.0	41.0
38	38.22517394954396	40.0	37.0	41.0	35.0	41.0
39	38.07200941959903	40.0	37.0	41.0	35.0	41.0
40	37.89938673003315	40.0	37.0	41.0	34.0	41.0
41	37.70720983508587	40.0	37.0	41.0	34.0	41.0
42	37.50120946513183	40.0	36.0	41.0	33.0	41.0
43	36.69256819248994	40.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	3.0
18	0.0
19	10.0
20	18.0
21	36.0
22	76.0
23	100.0
24	175.0
25	273.0
26	471.0
27	697.0
28	1030.0
29	1459.0
30	1966.0
31	2482.0
32	3137.0
33	4003.0
34	5989.0
35	8462.0
36	12465.0
37	30509.0
38	55404.0
39	152345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96055720770073	18.514776818110672	13.94904594544601	24.575620028742584
2	18.83670797820117	20.735923960215715	35.2302963901023	25.197071671480813
3	18.788685097966678	24.94166109364106	29.598457576231873	26.671196232160387
4	12.986453990523486	17.92569615390088	35.79305340144282	33.294796454132815
5	12.937719660211444	39.15572219297372	33.38906358940793	14.517494557406907
6	28.99585936054867	36.1946669702187	18.48525163989243	16.324222029340202
7	28.94925938046927	31.454986553593535	21.42069465985572	18.175059406081477
8	29.543675920260675	31.580557492280764	18.911410236343716	19.964356351114844
9	28.280852032612874	14.022681028472231	20.10344484127549	37.59302209763941
10	19.621081688697902	26.86364347813714	29.465060686691615	24.05021414647334
11	33.05823930334809	25.43683034761451	19.65060686691615	21.85432348212126
12	22.88663754464349	29.536205694446423	26.35851392307802	21.218642837832068
13	30.914995944734557	20.200202051822025	25.474181476685782	23.410620526757636
14	22.82118413750907	21.91550818879039	26.21551245749086	29.047795216209675
15	27.78710567879452	26.850481651702502	22.482889625634968	22.879523043868012
16	23.74891503863174	26.125158297642255	26.46807723502042	23.65784942870559
17	20.833392620839795	29.059178417450447	26.524281791146713	23.58314717056304
18	22.012621124375702	25.12521521364846	28.691003002319327	24.17116065965651
19	22.51383770400831	27.36343715761465	29.91683148593463	20.20589365244241
20	23.14596109790976	25.103871711322018	30.36540075982868	21.384766430939543
21	24.56814980292833	24.923874841702357	28.73724725735995	21.77072809800936
22	24.844903883094524	24.54182615005905	27.30047382575165	23.31279614109478
23	22.37510493888644	25.9999430839938	28.294013859047514	23.330938118072257
24	21.32891759985202	26.410449778739025	28.558317562856615	23.702315058552344
25	22.158468390273054	25.6491981957626	28.713057954723315	23.479275459241027
26	22.847152065339575	26.087095718493437	28.275160431992486	22.790591784174506
27	21.952859317861666	26.049033139344612	27.440629491028613	24.55747805176511
28	21.226824513723873	25.941959902673627	30.402751888899953	22.428463694702543
29	22.027561576004214	25.830262240498588	30.412356464946853	21.72981971855035
30	21.173110032868994	27.083481552099485	30.573144182472717	21.170264232558804
31	22.151353889497575	27.36948448327381	28.74009305767014	21.739068569558473
32	20.45063247911894	26.921270934418533	30.423028216110076	22.20506837035245
33	20.73129953471165	26.11946669702187	30.715434197982326	22.43379957028415
34	22.706640675023834	25.641372244909576	28.679975526117335	22.972011553949258
35	21.628082357460976	27.146444883962495	28.938943354344826	22.286529404231707
36	20.490829408500407	26.904551857596154	28.74507320821298	23.85954552569046
37	21.023349791545126	25.826704990110844	29.66604533359894	23.48389988474509
38	20.579760668193913	25.05727173124262	30.868751689693934	23.494215910869535
39	20.63098507377737	25.13232971442394	31.33261714025527	22.90406807154342
40	20.44885385392507	24.626132984248493	31.27534540901265	23.649667752813784
41	18.742085117887278	24.997509924728583	32.11236642524794	24.1480385321362
42	18.484895914853656	25.170392293572757	31.750594060814752	24.594117730758832
43	18.279286842442268	25.488766203275514	32.51220136882995	23.719745585452266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	22.0
1	39.0
2	56.0
3	177.5
4	299.0
5	299.0
6	417.0
7	535.0
8	550.5
9	566.0
10	901.5
11	1237.0
12	1237.0
13	2206.0
14	3175.0
15	4997.0
16	6819.0
17	6062.0
18	5305.0
19	5305.0
20	5732.5
21	6160.0
22	4268.5
23	2377.0
24	2090.0
25	1803.0
26	1803.0
27	1977.5
28	2152.0
29	3053.5
30	3955.0
31	4787.0
32	5619.0
33	5619.0
34	7058.5
35	8498.0
36	10281.0
37	12064.0
38	13359.5
39	14655.0
40	14655.0
41	16504.0
42	18353.0
43	20322.0
44	22291.0
45	23725.5
46	25160.0
47	25160.0
48	27660.5
49	30161.0
50	28913.5
51	27666.0
52	23816.0
53	19966.0
54	19966.0
55	17393.5
56	14821.0
57	12485.5
58	10150.0
59	9755.0
60	9360.0
61	9360.0
62	8376.0
63	7392.0
64	6484.5
65	5577.0
66	4983.5
67	4390.0
68	4390.0
69	3744.0
70	3098.0
71	2738.5
72	2379.0
73	1962.5
74	1546.0
75	1546.0
76	1264.5
77	983.0
78	880.0
79	777.0
80	687.5
81	598.0
82	598.0
83	541.0
84	484.0
85	394.5
86	305.0
87	249.5
88	194.0
89	194.0
90	146.5
91	99.0
92	72.5
93	46.0
94	33.5
95	21.0
96	21.0
97	11.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	281116.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.238651991129224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.84254461442875	27.484315804039756
2	9.040815513785773	6.552539342401696
3	3.8245465328925725	4.157892324881214
4	2.187267898488081	3.1705456073871257
5	1.5304674657146058	2.773103893688855
6	1.0986168388268702	2.388743597630487
7	0.8656980301464565	2.1960210750718474
8	0.7267464965354618	2.10690506989768
9	0.5158200717225976	1.6823361662275076
>10	3.9016390308313116	27.798395359618382
>50	0.34587935041442947	8.696779574102498
>100	0.11396024840244208	6.6945676898160595
>500	0.0019993026035516155	0.5169452431475929
>1k	0.003998605207103231	3.7809092520892977
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3606	1.2827444898191493	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3063	1.0895857937648514	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2696	0.9590347045347829	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1072	0.3813372415657593	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	877	0.31197085900482363	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	550	0.19564877132571606	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	419	0.14904879124631826	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	393	0.13979994023819348	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.12877246403619858	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.12770528891987648	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	357	0.12699383884232843	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	343	0.12201368829949201	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	342	0.121657963260718	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	330	0.11738926279542963	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	329	0.11703353775665562	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	294	0.10458316139956458	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	288	0.10244881116692041	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	283	0.10067018597305027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.557250387740292E-4	0.0	0.0	0.0
3	0.0	3.557250387740292E-4	0.0	0.0	0.0
4	0.0	3.557250387740292E-4	0.0	0.0	0.0
5	0.0	3.557250387740292E-4	0.0	0.0	0.0
6	0.0	3.557250387740292E-4	0.0	0.0	0.0
7	0.0	3.557250387740292E-4	0.0	0.0	0.0
8	0.0	3.557250387740292E-4	0.0	0.0	0.0
9	0.0	3.557250387740292E-4	0.0	0.0	0.0
10	0.0	3.557250387740292E-4	0.0	3.557250387740292E-4	0.0
11	0.0	3.557250387740292E-4	0.0	0.0010671751163220877	3.557250387740292E-4
12	0.0	3.557250387740292E-4	0.0	0.0014229001550961169	3.557250387740292E-4
13	0.0	3.557250387740292E-4	0.0	0.001778625193870146	3.557250387740292E-4
14	0.0	3.557250387740292E-4	0.0	0.0021343502326441753	3.557250387740292E-4
15	0.0	3.557250387740292E-4	0.0	0.0049801505428364095	3.557250387740292E-4
16	0.0	3.557250387740292E-4	0.0	0.009604576046898789	3.557250387740292E-4
17	0.0	3.557250387740292E-4	0.0	0.016363351783605345	3.557250387740292E-4
18	0.0	3.557250387740292E-4	0.0	0.01849770201624952	3.557250387740292E-4
19	0.0	3.557250387740292E-4	0.0	0.021699227365215783	3.557250387740292E-4
20	0.0	3.557250387740292E-4	0.0	0.023477852559085927	3.557250387740292E-4
21	0.0	3.557250387740292E-4	0.0	0.026323652869278163	3.557250387740292E-4
22	0.0	3.557250387740292E-4	0.0	0.03450532876108083	3.557250387740292E-4
23	0.0	3.557250387740292E-4	0.0	0.04802288023449395	3.557250387740292E-4
24	0.0	3.557250387740292E-4	0.0	0.07292363294867599	3.557250387740292E-4
25	0.0	3.557250387740292E-4	0.0	0.07648088333641628	3.557250387740292E-4
26	0.0	3.557250387740292E-4	0.0	0.08821980961595925	3.557250387740292E-4
27	0.0	3.557250387740292E-4	0.0	0.11596636264033353	3.557250387740292E-4
28	0.0	7.114500775480584E-4	0.0	0.21023349791545126	3.557250387740292E-4
29	0.0	7.114500775480584E-4	0.0	0.37564564094537484	3.557250387740292E-4
30	0.0	7.114500775480584E-4	0.0	0.5947722648301769	3.557250387740292E-4
31	0.0	7.114500775480584E-4	0.0	1.2101765819092474	3.557250387740292E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAACC	30	3.5926243E-4	30.833334	26
CCCGTTG	30	3.5926243E-4	30.833334	31
TAACCCG	30	3.5926243E-4	30.833334	28
ACCCGTT	30	3.5926243E-4	30.833334	30
GTAACCC	30	3.5926243E-4	30.833334	27
CTCACGG	25	0.005489371	29.599998	15
CCATGCT	40	5.924422E-5	27.75	9
GAGACAC	40	5.924422E-5	27.75	14
AGACACC	40	5.924422E-5	27.75	15
GCGGGTA	35	8.852666E-4	26.428572	23
GATAGAG	50	9.059229E-6	25.899998	7
CCCTAAG	45	1.319741E-4	24.666666	1
TAAGATA	45	1.319741E-4	24.666666	4
CGGGTAA	40	0.0019275799	23.125	24
TATGAGT	40	0.0019275799	23.125	4
CCTTTCC	40	0.0019275799	23.125	37
AACGCCG	40	0.0019275799	23.125	5
TCATGGA	40	0.0019275799	23.125	2
TAGAGGA	50	2.6950723E-4	22.199999	9
GACACCT	50	2.6950723E-4	22.199999	16
>>END_MODULE