FastQCFastQC Report
Fri 10 Feb 2017
ERR1631512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143943
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1720.11949174325948467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAAAG250.005480577229.5999981
GGTGAAG358.831587E-426.428573
GGTATCA659.273026E-825.6153851
AAGACGG451.3153697E-424.6666685
ATCGAGA551.887707E-523.54545423
CGAAAGC400.001923032823.12500219
GCGAAAG400.001923032823.12500218
AGACGGA400.001923032823.1250026
AGCATTT400.001923032823.12500223
ACGGACC400.001923032823.1250028
CGAGCCG400.001923032823.12500215
TATACAC1500.022.237
GACCAGA450.003809807620.55555711
GCATTTG450.003809807620.55555724
AAGCATT450.003809807620.55555722
AGTGACC450.003809807620.55555734
GAGCGAA450.003809807620.55555716
GTATCAA909.334508E-820.5555572
CGCAAGA450.003809807620.5555572
AGAGCGA450.003809807620.55555715
Produced by FastQC (version 0.11.5)