FastQCFastQC Report
Fri 10 Feb 2017
ERR1631511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49263
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17593.5706311024501147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13662.772872135273938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11762.3871871384203156No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.9459432028094107No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3790.7693400726711731No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2410.489210969693279No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.4019243651421959No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1950.39583460203398085No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1800.36538578649290543No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1760.35726610234861866No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1650.33493697095182995No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1640.33290704991575826No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1610.3268172868075432No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1500.3044881554107545No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1470.29839839230253945No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.294338550230396No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1420.28824878712218094No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.28215902401396586No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1380.2801291029778942No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1330.26997949779753566No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1250.2537401295089621No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1210.2456204453646753No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.2395306822564602No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1070.21720155085967158No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.21314170878752817No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1050.21314170878752817No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1040.21111178775145645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.1948724194628829No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.1908125773907395No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT890.18066297221038102No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.1766031301382376No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT860.17457320910216592No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC860.17457320910216592No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC850.17254328806609423No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT790.16036376184966403No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT770.15630391977752067No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC680.13803463045287537No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT670.1360047094168037No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA670.1360047094168037No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG650.1319448673446603No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.12788502527251688No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT600.12179526216430181No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT570.11570549905608671No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA560.11367557802001503No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT550.11164565698394333No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG550.11164565698394333No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10961573594787163No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10758581491179993No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC520.10555589387572822No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT520.10555589387572822No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA510.10352597283965655No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA510.10352597283965655ABI Solid3 Adapter B (95% over 23bp)
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA500.10149605180358484ABI Solid3 Adapter B (100% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC352.3425546E-531.71428537
GGGTATC250.00544604329.61
TGCTGTC250.00544604329.637
TCCTGTC400.001905205923.12500234
AGGGCAA502.65157E-422.210
GAGGGCA502.65157E-422.29
TGGGCAG2500.021.466
GGGCAGT2650.020.9433967
AGTCGGT2650.020.94339611
GGCAGTC2650.020.9433968
CAGTCGG2700.020.55555510
TATACAC450.003774826620.55555537
GCAGTCG2700.020.5555559
ACAGTGG555.0476915E-420.1818183
GGGCAAA555.0476915E-420.18181811
AACAGTG555.0476915E-420.1818182
TCCTCCG656.732323E-519.92307721
GGCAAAA656.732323E-519.92307712
CCTCCGC656.732323E-519.92307722
TCCGCTT656.732323E-519.92307724
Produced by FastQC (version 0.11.5)