##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631511.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	49263
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.95042932829913	34.0	33.0	34.0	31.0	34.0
2	33.02675435925543	34.0	33.0	34.0	31.0	34.0
3	33.0011367557802	34.0	33.0	34.0	31.0	34.0
4	36.344457300611005	37.0	37.0	37.0	35.0	37.0
5	36.38533991027749	37.0	37.0	37.0	35.0	37.0
6	36.47983273450663	37.0	37.0	37.0	35.0	37.0
7	36.48151756896657	37.0	37.0	37.0	35.0	37.0
8	36.523110650995676	37.0	37.0	37.0	35.0	37.0
9	38.307878123540995	39.0	39.0	39.0	37.0	39.0
10	38.298256297830015	39.0	39.0	39.0	37.0	39.0
11	38.3516432210787	39.0	39.0	39.0	37.0	39.0
12	38.335566246473014	39.0	39.0	39.0	37.0	39.0
13	38.356535330775635	39.0	39.0	39.0	37.0	39.0
14	39.71416681891075	41.0	40.0	41.0	37.0	41.0
15	39.65889206909851	41.0	39.0	41.0	37.0	41.0
16	39.542821184255935	41.0	39.0	41.0	37.0	41.0
17	39.58187686498995	41.0	39.0	41.0	37.0	41.0
18	39.75847999512819	41.0	40.0	41.0	38.0	41.0
19	39.803260053183934	41.0	40.0	41.0	38.0	41.0
20	39.82522379879423	41.0	40.0	41.0	38.0	41.0
21	39.81864685463736	41.0	40.0	41.0	38.0	41.0
22	39.73006110062319	41.0	40.0	41.0	37.0	41.0
23	39.64462172421493	41.0	40.0	41.0	37.0	41.0
24	39.611899397113454	41.0	40.0	41.0	37.0	41.0
25	39.592513651218965	41.0	40.0	41.0	36.0	41.0
26	39.383492682134666	41.0	39.0	41.0	35.0	41.0
27	39.24624972088586	41.0	39.0	41.0	35.0	41.0
28	39.06660170919351	41.0	39.0	41.0	35.0	41.0
29	38.830034711649716	40.0	38.0	41.0	35.0	41.0
30	38.54623145159653	40.0	37.0	41.0	35.0	41.0
31	38.206564764630656	40.0	36.0	41.0	35.0	41.0
32	37.92657775612528	40.0	35.0	41.0	35.0	41.0
33	37.713070661551264	40.0	35.0	41.0	35.0	41.0
34	37.511763392404035	40.0	35.0	41.0	35.0	41.0
35	37.30489413961797	40.0	35.0	41.0	34.0	41.0
36	37.0674339768183	40.0	35.0	41.0	33.0	41.0
37	36.83111056979884	39.0	35.0	41.0	33.0	41.0
38	36.57948155816739	39.0	35.0	41.0	33.0	41.0
39	36.22641739236344	39.0	35.0	41.0	33.0	41.0
40	35.866167306091796	39.0	35.0	41.0	31.0	41.0
41	35.425207559425935	38.0	35.0	41.0	27.0	41.0
42	34.93916326654893	38.0	35.0	41.0	21.0	41.0
43	33.99959401579279	37.0	35.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	1.0
14	0.0
15	1.0
16	2.0
17	1.0
18	0.0
19	2.0
20	8.0
21	11.0
22	14.0
23	30.0
24	49.0
25	84.0
26	128.0
27	198.0
28	325.0
29	444.0
30	586.0
31	663.0
32	853.0
33	1061.0
34	1388.0
35	1977.0
36	2999.0
37	9228.0
38	9447.0
39	19761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.1773947993423	13.716176440736454	16.58242494366969	22.524003816251547
2	19.24568134299576	22.079451109351847	33.71089864604267	24.963968901609725
3	19.72880254958082	25.197409820757972	30.30266122647829	24.771126403182915
4	12.2140348740434	19.235531737815396	30.806081643424072	37.74435174471713
5	12.301321478594483	42.179729208533786	28.891866106408457	16.62708320646327
6	27.068997016016077	31.2770233237927	22.008403873089335	19.645575787101883
7	29.480543206869253	33.89359153928912	17.524308304406958	19.101556949434666
8	37.33024785335851	27.541968617420782	16.525587154659686	18.602196374561032
9	32.7405963908004	12.02322229665266	17.05945638714654	38.1767249254004
10	21.76075350668859	29.431825102003533	21.005622881269918	27.801798510037962
11	32.30822321011713	27.90938432494976	14.64182043318515	25.140572031747965
12	25.04719566408867	32.63707041796074	23.90029027870816	18.415443639242433
13	35.95193146986582	18.93916326654893	25.14869171589225	19.960213547692994
14	23.944948541501734	22.554452631792625	26.695491545378886	26.805107281326755
15	32.33664210462213	25.140572031747965	21.809471611554308	20.713314252075595
16	21.752633822544304	26.297627022308834	27.704362300306517	24.245376854840348
17	17.242149280392994	32.06869252786066	27.85457645697582	22.834581734770516
18	21.334470089113534	22.88532976067231	31.873820108397783	23.906380041816373
19	20.021111178775143	30.43663601485902	33.670300225321235	15.871952581044596
20	23.435438361447737	23.049753364594117	35.62511418305828	17.889694090899862
21	26.673162413982098	22.09366055660435	32.785254653593974	18.44792237581958
22	24.543775247142886	23.490246229421675	28.89795586951668	23.068022653918764
23	20.68489535757059	26.372734100643484	29.476483364797108	23.465887176988815
24	19.18681363294968	26.29559710127276	34.666991454032434	19.850597811745125
25	18.606256216633174	25.749548342569472	31.126809167123398	24.517386273673957
26	22.7209061567505	24.78736577147149	30.763453301666566	21.72827477011144
27	20.64429693684916	24.700079166920407	29.59421878488927	25.061405111341166
28	17.843005907070214	26.157562470819883	33.215597913241176	22.783833708868727
29	18.671213689787468	26.898483648986055	34.87810324178389	19.552199419442584
30	17.57302640927268	28.01494021882549	37.1008667762824	17.311166595619433
31	18.194182246310618	29.683535310476422	32.231086210746405	19.891196232466555
32	17.254328806609422	28.938554290238113	36.349795992935874	17.45732091021659
33	19.164484501552888	27.330856829669326	35.57436615715648	17.9302925116213
34	19.82217891724012	28.682784239693078	30.31484075269472	21.180196090372082
35	17.77804843391592	30.39806751517366	30.751273775450134	21.072610275460285
36	15.342143190629884	29.799240809532506	32.821793232243266	22.03682276759434
37	16.361163550737874	29.96163449241824	33.01666565170615	20.66053630513773
38	17.6359539613909	25.591214501755882	35.30641658039502	21.466414956458195
39	18.693542821184256	25.566855449323022	35.24957879138502	20.490022938107707
40	17.76383898666342	25.118242900351177	32.8197633112072	24.29815480177821
41	14.158699226600085	26.6224143880803	36.301077888070154	22.917808497249457
42	14.731136958772304	25.52016726549337	34.86186387349532	24.886831902239003
43	13.300042628341757	28.083957534051923	37.068388039705255	21.547611797901062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	55.5
4	99.0
5	99.0
6	167.0
7	235.0
8	237.0
9	239.0
10	372.0
11	505.0
12	505.0
13	960.5
14	1416.0
15	2309.5
16	3203.0
17	2785.0
18	2367.0
19	2367.0
20	2573.0
21	2779.0
22	1922.0
23	1065.0
24	890.5
25	716.0
26	716.0
27	647.5
28	579.0
29	633.0
30	687.0
31	670.0
32	653.0
33	653.0
34	624.5
35	596.0
36	573.5
37	551.0
38	625.0
39	699.0
40	699.0
41	883.0
42	1067.0
43	1444.5
44	1822.0
45	2389.0
46	2956.0
47	2956.0
48	4324.0
49	5692.0
50	5347.5
51	5003.0
52	4037.0
53	3071.0
54	3071.0
55	2382.0
56	1693.0
57	1634.5
58	1576.0
59	1586.5
60	1597.0
61	1597.0
62	1607.0
63	1617.0
64	1630.0
65	1643.0
66	1550.5
67	1458.0
68	1458.0
69	1322.5
70	1187.0
71	1089.0
72	991.0
73	841.5
74	692.0
75	692.0
76	544.0
77	396.0
78	301.5
79	207.0
80	162.0
81	117.0
82	117.0
83	79.5
84	42.0
85	34.0
86	26.0
87	15.5
88	5.0
89	5.0
90	2.5
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	49263.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.44142662850415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78117401875652	52.46939894038122
2	5.227509551927753	6.110062318575808
3	1.6255644320944773	2.850009134644662
4	0.868357068426537	2.0299210360716966
5	0.5001736714136853	1.4615431459716217
6	0.3647099687391455	1.278850252725169
7	0.20840569642236886	0.8525668351501127
8	0.18756512678013199	0.8769258875829731
9	0.1528308440430705	0.8038487302843919
>10	0.9030913511635985	10.744372043927491
>50	0.0868357068426537	3.461015366502243
>100	0.08336227856894755	8.330795932038244
>500	0.0	0.0
>1k	0.010420284821118444	8.730690376144368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1759	3.5706311024501147	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1366	2.772872135273938	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1176	2.3871871384203156	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	466	0.9459432028094107	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	379	0.7693400726711731	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	241	0.489210969693279	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198	0.4019243651421959	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	195	0.39583460203398085	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	180	0.36538578649290543	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	176	0.35726610234861866	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	165	0.33493697095182995	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	164	0.33290704991575826	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	161	0.3268172868075432	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	150	0.3044881554107545	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	147	0.29839839230253945	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.294338550230396	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	142	0.28824878712218094	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139	0.28215902401396586	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	138	0.2801291029778942	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	133	0.26997949779753566	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	125	0.2537401295089621	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	121	0.2456204453646753	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118	0.2395306822564602	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	107	0.21720155085967158	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105	0.21314170878752817	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	105	0.21314170878752817	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	104	0.21111178775145645	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	96	0.1948724194628829	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	94	0.1908125773907395	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	89	0.18066297221038102	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.1766031301382376	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	86	0.17457320910216592	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	86	0.17457320910216592	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	85	0.17254328806609423	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	79	0.16036376184966403	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	77	0.15630391977752067	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	68	0.13803463045287537	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	67	0.1360047094168037	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	67	0.1360047094168037	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	65	0.1319448673446603	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.12788502527251688	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	60	0.12179526216430181	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	57	0.11570549905608671	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	56	0.11367557802001503	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	55	0.11164565698394333	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	55	0.11164565698394333	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.10961573594787163	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.10758581491179993	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	52	0.10555589387572822	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	52	0.10555589387572822	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	51	0.10352597283965655	No Hit
ATCACCGACTGCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAA	51	0.10352597283965655	ABI Solid3 Adapter B (95% over 23bp)
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	50	0.10149605180358484	ABI Solid3 Adapter B (100% over 21bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.002029921036071697	0.0
12	0.0	0.0	0.0	0.002029921036071697	0.0
13	0.0	0.0	0.0	0.002029921036071697	0.0
14	0.0	0.0	0.0	0.004059842072143394	0.0
15	0.0	0.0	0.0	0.00608976310821509	0.0
16	0.0	0.0	0.0	0.01826928932464527	0.0
17	0.0	0.0	0.0	0.030448815541075452	0.0
18	0.0	0.0	0.0	0.03247873657714715	0.0
19	0.0	0.0	0.0	0.03450865761321885	0.0
20	0.0	0.0	0.0	0.03653857864929054	0.0
21	0.0	0.0	0.0	0.03856849968536224	0.0
22	0.0	0.0	0.0	0.05074802590179242	0.0
23	0.0	0.0	0.0	0.0690173152264377	0.0
24	0.0	0.0	0.0	0.10961573594787163	0.0
25	0.0	0.0	0.0	0.1299149463085886	0.0
26	0.0	0.0	0.0	0.15224407770537726	0.0
27	0.0	0.0	0.0	0.20908186671538478	0.0
28	0.0	0.0	0.0	0.3410267340600451	0.0
29	0.0	0.0	0.0	0.6800235470840185	0.0
30	0.0	0.0	0.0	1.1083368856951465	0.0
31	0.0	0.0	0.0	2.0035320626027646	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCCC	35	2.3425546E-5	31.714285	37
GGGTATC	25	0.005446043	29.6	1
TGCTGTC	25	0.005446043	29.6	37
TCCTGTC	40	0.0019052059	23.125002	34
AGGGCAA	50	2.65157E-4	22.2	10
GAGGGCA	50	2.65157E-4	22.2	9
TGGGCAG	250	0.0	21.46	6
GGGCAGT	265	0.0	20.943396	7
AGTCGGT	265	0.0	20.943396	11
GGCAGTC	265	0.0	20.943396	8
CAGTCGG	270	0.0	20.555555	10
TATACAC	45	0.0037748266	20.555555	37
GCAGTCG	270	0.0	20.555555	9
ACAGTGG	55	5.0476915E-4	20.181818	3
GGGCAAA	55	5.0476915E-4	20.181818	11
AACAGTG	55	5.0476915E-4	20.181818	2
TCCTCCG	65	6.732323E-5	19.923077	21
GGCAAAA	65	6.732323E-5	19.923077	12
CCTCCGC	65	6.732323E-5	19.923077	22
TCCGCTT	65	6.732323E-5	19.923077	24
>>END_MODULE