FastQCFastQC Report
Fri 10 Feb 2017
ERR1631510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114515
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.3353272497052788No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC3090.2698336462472165No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT2940.25673492555560407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2880.2514954372789591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2220.1938610662358643No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1730.15107191197659695No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1620.1414661834694145No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT1560.1362266951927695No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT1490.13011395887001703No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1370.11963498231672706No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1360.11876173427061956No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG1340.11701523817840458No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG1300.11352224599397459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.1082827577173296No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG1230.1074095096712221No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG1210.10566301357900712No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT1190.10391651748679212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGT405.8942525E-527.7511
CAGTCGG451.3130749E-424.66666810
TTATTGA451.3130749E-424.66666829
ATACACA601.3199206E-624.66666837
GGTATCA1350.023.2962971
TTGATAT400.001920644423.12532
GCAGTCG502.6815696E-422.1999999
CTTATTG502.6815696E-422.19999928
TGCATCA956.9776434E-921.42105314
TATGCCC450.00380512120.55555736
GATATGC450.00380512120.55555734
GCATCAG1001.2596502E-820.34999815
TCAGAGG555.1043276E-420.181823
TATACAC1202.3101165E-1020.04166837
ACAGGCT1152.9667717E-919.3043488
GGCAGTC609.1677625E-418.58
TATTGAT609.1677625E-418.530
CAGGACA1306.7484507E-1018.54
TGATATG500.00699720918.49999833
ATATGCC500.00699720918.49999835