##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631510.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114515
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.002218050037115	34.0	33.0	34.0	31.0	34.0
2	33.15128149150766	34.0	33.0	34.0	31.0	34.0
3	33.21941230406497	34.0	33.0	34.0	31.0	34.0
4	36.44813343230145	37.0	37.0	37.0	35.0	37.0
5	36.43632711871807	37.0	37.0	37.0	35.0	37.0
6	36.48010304326944	37.0	37.0	37.0	35.0	37.0
7	36.51428197179409	37.0	37.0	37.0	35.0	37.0
8	36.50437933895123	37.0	37.0	37.0	35.0	37.0
9	38.35114177182029	39.0	39.0	39.0	37.0	39.0
10	38.32680434877527	39.0	39.0	39.0	37.0	39.0
11	38.378666550233596	39.0	39.0	39.0	37.0	39.0
12	38.346269047723005	39.0	39.0	39.0	37.0	39.0
13	38.36376020608654	39.0	39.0	39.0	37.0	39.0
14	39.89350740077719	41.0	40.0	41.0	38.0	41.0
15	39.88225123346287	41.0	40.0	41.0	38.0	41.0
16	39.824171505916254	41.0	40.0	41.0	38.0	41.0
17	39.834772737196	41.0	40.0	41.0	38.0	41.0
18	39.73960616513121	41.0	40.0	41.0	38.0	41.0
19	39.754521241758724	41.0	40.0	41.0	38.0	41.0
20	39.70975854691525	41.0	40.0	41.0	37.0	41.0
21	39.700676767235734	41.0	40.0	41.0	37.0	41.0
22	39.63444090293848	41.0	40.0	41.0	37.0	41.0
23	39.586979871632536	41.0	40.0	41.0	37.0	41.0
24	39.556590839627994	41.0	40.0	41.0	37.0	41.0
25	39.562668646028904	41.0	40.0	41.0	37.0	41.0
26	39.43713923940095	41.0	39.0	41.0	36.0	41.0
27	39.40840064620355	41.0	39.0	41.0	36.0	41.0
28	39.33882897437017	41.0	39.0	41.0	36.0	41.0
29	39.292904859625374	41.0	39.0	41.0	35.0	41.0
30	39.21211195039951	41.0	39.0	41.0	35.0	41.0
31	39.16755010260665	41.0	39.0	41.0	35.0	41.0
32	39.10412609701786	41.0	39.0	41.0	35.0	41.0
33	39.01260096930533	41.0	39.0	41.0	35.0	41.0
34	38.93342356896476	41.0	39.0	41.0	35.0	41.0
35	38.823245862987385	40.0	38.0	41.0	35.0	41.0
36	38.75573505654281	40.0	38.0	41.0	35.0	41.0
37	38.67482862507095	40.0	38.0	41.0	35.0	41.0
38	38.60185128585775	40.0	38.0	41.0	35.0	41.0
39	38.52065668253067	40.0	38.0	41.0	35.0	41.0
40	38.40363271187181	40.0	38.0	41.0	35.0	41.0
41	38.29872069161245	40.0	37.0	41.0	34.0	41.0
42	38.16473824389818	40.0	37.0	41.0	34.0	41.0
43	37.34403353272497	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	5.0
19	6.0
20	5.0
21	13.0
22	31.0
23	43.0
24	75.0
25	109.0
26	158.0
27	230.0
28	374.0
29	484.0
30	657.0
31	889.0
32	1147.0
33	1542.0
34	2371.0
35	3779.0
36	5278.0
37	9659.0
38	21223.0
39	66435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.523424878836835	16.496528839016722	11.513775487927346	27.4662707942191
2	20.450595991791467	18.52421080207833	32.35384010828275	28.671353097847447
3	21.624241365759943	19.84980133606951	27.659258612408856	30.86669868576169
4	16.261625114613807	14.458367899401825	30.59861153560669	38.68139545037768
5	17.760992009780377	33.78160066366851	30.578526830546217	17.87888049600489
6	37.37588962144697	30.722612758153957	15.05654281098546	16.844954809413615
7	32.210627428721125	28.053093481203334	18.80277692878662	20.933502161288914
8	30.361961315111557	29.97685892677815	18.305898790551456	21.355280967558834
9	28.557830851853467	13.379033314412958	17.63873728332533	40.42439855040824
10	20.798148714142254	24.22040780683753	28.7359734532594	26.24547002576082
11	38.21857398594071	20.158057896345458	18.877876260751865	22.74549185696197
12	25.03864122604026	23.391695411081518	25.450814303802993	26.118849059075227
13	31.878793171200282	17.201240012225472	23.23014452255163	27.689822294022616
14	24.735624154040956	18.76959350303454	22.408418111164476	34.08636423176003
15	28.067938697987167	24.299873379033315	20.05588787495088	27.57630004802864
16	27.520412173077762	23.075579618390606	21.385844649172597	28.01816355935904
17	26.352879535432038	23.950574160590314	22.85028162249487	26.846264681482772
18	26.033270750556696	21.61550888529887	24.243985504082435	28.107234860062004
19	27.122211064052742	22.934986682967295	23.45282277430904	26.489979478670918
20	27.044491987949176	22.367375452997422	23.499978168798847	27.088154390254555
21	29.44330437060647	22.076583853643626	22.601405929354232	25.87870584639567
22	29.194428677465833	22.42850281622495	21.97790682443348	26.39916168187574
23	27.716892983451952	22.500109156005763	22.223289525389685	27.5597083351526
24	27.1632537222198	22.59180020084705	22.824084181111644	27.420861895821506
25	26.98074488058333	21.93249792603589	23.187355368292366	27.899401825088415
26	27.67497707723879	22.68873073396498	23.47203423132341	26.16425795747282
27	28.369209273894246	21.86700432257783	22.345544251844736	27.418242151683188
28	26.40964065842903	21.95782211937301	24.174125660393837	27.45841156180413
29	26.10051085010697	22.135091472732828	24.624721652185304	27.139676024974897
30	25.77304283281666	22.671265773042833	24.478015980439245	27.077675413701265
31	26.479500502117624	22.567349255556042	23.500851416844956	27.452298825481382
32	25.820198227306467	22.452080513469852	24.339169541108152	27.38855171811553
33	26.451556564642186	22.135091472732828	23.823953193904728	27.58939876872025
34	26.730995939396585	22.436362048639914	24.016067764048376	26.81657424791512
35	26.69344627341396	22.10278129502685	24.94607693315286	26.257695498406324
36	25.97825612365192	22.21193730079029	23.864122604025674	27.945683971532116
37	25.206304850892895	21.536043313103086	25.38619394839104	27.871457887612976
38	25.379207964022182	20.951840370257173	26.511810679823604	27.157140985897044
39	24.31122560363271	20.850543596908704	26.919617517355803	27.918613282102783
40	24.38545168755185	20.48814565777409	27.888922848535124	27.237479806138932
41	22.97864908527267	20.717809893900363	28.75256516613544	27.550975854691522
42	23.291271885779157	20.790289481727285	28.315067895035583	27.60337073745798
43	22.199711828144785	19.696109679954592	29.211020390341876	28.893158101558747
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	40.5
2	28.0
3	44.0
4	60.0
5	60.0
6	77.0
7	94.0
8	85.5
9	77.0
10	115.5
11	154.0
12	154.0
13	263.5
14	373.0
15	555.5
16	738.0
17	634.0
18	530.0
19	530.0
20	624.5
21	719.0
22	610.5
23	502.0
24	564.5
25	627.0
26	627.0
27	693.5
28	760.0
29	978.0
30	1196.0
31	1389.0
32	1582.0
33	1582.0
34	1921.5
35	2261.0
36	2698.0
37	3135.0
38	3472.5
39	3810.0
40	3810.0
41	4126.5
42	4443.0
43	4848.5
44	5254.0
45	5589.0
46	5924.0
47	5924.0
48	6491.0
49	7058.0
50	7484.5
51	7911.0
52	8504.5
53	9098.0
54	9098.0
55	9072.0
56	9046.0
57	8882.5
58	8719.0
59	8218.5
60	7718.0
61	7718.0
62	7527.0
63	7336.0
64	6686.5
65	6037.0
66	5395.0
67	4753.0
68	4753.0
69	4172.5
70	3592.0
71	3302.5
72	3013.0
73	2911.0
74	2809.0
75	2809.0
76	2645.5
77	2482.0
78	1966.5
79	1451.0
80	971.5
81	492.0
82	492.0
83	393.5
84	295.0
85	244.0
86	193.0
87	157.5
88	122.0
89	122.0
90	85.5
91	49.0
92	30.0
93	11.0
94	7.5
95	4.0
96	4.0
97	4.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	114515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.23822206697812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.71317968340095	61.901060996376025
2	4.564708291209706	6.2297515609308824
3	1.4537450571388353	2.9760293411343492
4	0.8113330688609345	2.214557044928612
5	0.44661709942029354	1.523817840457582
6	0.3109683528914938	1.2731956512247304
7	0.25594103118641465	1.2225472645504956
8	0.17403990120676197	0.9500938741649565
9	0.14972550324405257	0.9195301925511941
>10	1.0058482525626096	13.802558616775096
>50	0.08574024544744892	3.7698118150460638
>100	0.028153513430505615	3.217045801860018
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	384	0.3353272497052788	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	309	0.2698336462472165	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	294	0.25673492555560407	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.2514954372789591	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.1938610662358643	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	173	0.15107191197659695	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	162	0.1414661834694145	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	156	0.1362266951927695	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	149	0.13011395887001703	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	137	0.11963498231672706	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	136	0.11876173427061956	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	134	0.11701523817840458	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	130	0.11352224599397459	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	124	0.1082827577173296	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	123	0.1074095096712221	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	121	0.10566301357900712	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	119	0.10391651748679212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.732480461074968E-4	0.0
2	0.0	0.0	0.0	8.732480461074968E-4	0.0
3	0.0	0.0	0.0	8.732480461074968E-4	0.0
4	0.0	0.0	0.0	8.732480461074968E-4	0.0
5	0.0	0.0	0.0	8.732480461074968E-4	0.0
6	0.0	0.0	0.0	8.732480461074968E-4	0.0
7	0.0	0.0	0.0	8.732480461074968E-4	0.0
8	0.0	0.0	0.0	8.732480461074968E-4	0.0
9	0.0	0.0	0.0	8.732480461074968E-4	0.0
10	0.0	0.0	0.0	8.732480461074968E-4	0.0
11	0.0	0.0	0.0	8.732480461074968E-4	0.0
12	0.0	0.0	0.0	8.732480461074968E-4	0.0
13	0.0	0.0	0.0	8.732480461074968E-4	0.0
14	0.0	0.0	0.0	8.732480461074968E-4	0.0
15	0.0	0.0	0.0	8.732480461074968E-4	0.0
16	0.0	0.0	0.0	8.732480461074968E-4	0.0
17	0.0	0.0	0.0	8.732480461074968E-4	0.0
18	0.0	0.0	0.0	0.0017464960922149936	0.0
19	0.0	0.0	0.0	0.0017464960922149936	0.0
20	0.0	0.0	0.0	0.0026197441383224904	0.0
21	0.0	0.0	0.0	0.0034929921844299872	0.0
22	0.0	0.0	0.0	0.0069859843688599745	0.0
23	0.0	0.0	0.0	0.00785923241496747	0.0
24	0.0	0.0	0.0	0.01571846482993494	0.0
25	0.0	0.0	0.0	0.026197441383224905	0.0
26	0.0	0.0	0.0	0.04191590621315985	0.0
27	0.0	0.0	0.0	0.11876173427061958	0.0
28	0.0	0.0	0.0	0.3833558922411911	0.0
29	0.0	0.0	0.0	0.7291621184997599	0.0
30	0.0	0.0	0.0	1.140461948216391	0.0
31	0.0	0.0	0.0	1.8163559359035935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCGGT	40	5.8942525E-5	27.75	11
CAGTCGG	45	1.3130749E-4	24.666668	10
TTATTGA	45	1.3130749E-4	24.666668	29
ATACACA	60	1.3199206E-6	24.666668	37
GGTATCA	135	0.0	23.296297	1
TTGATAT	40	0.0019206444	23.125	32
GCAGTCG	50	2.6815696E-4	22.199999	9
CTTATTG	50	2.6815696E-4	22.199999	28
TGCATCA	95	6.9776434E-9	21.421053	14
TATGCCC	45	0.003805121	20.555557	36
GATATGC	45	0.003805121	20.555557	34
GCATCAG	100	1.2596502E-8	20.349998	15
TCAGAGG	55	5.1043276E-4	20.18182	3
TATACAC	120	2.3101165E-10	20.041668	37
ACAGGCT	115	2.9667717E-9	19.304348	8
GGCAGTC	60	9.1677625E-4	18.5	8
TATTGAT	60	9.1677625E-4	18.5	30
CAGGACA	130	6.7484507E-10	18.5	4
TGATATG	50	0.006997209	18.499998	33
ATATGCC	50	0.006997209	18.499998	35
>>END_MODULE