FastQCFastQC Report
Fri 10 Feb 2017
ERR1631509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325281
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.20780498626329058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20680.15604237893699524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19630.148119530876848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19300.021.6632121
CTCTATA759.267524E-619.7333342
CAGTCGG12150.019.18518410
GCAGTCG12100.019.111579
ATACACA7500.018.99333437
CTATACG500.007035437518.4999983
AGTCGGT13000.018.35769311
ATAACGA1451.546141E-1017.86206812
GCTCTAT1258.581992E-917.761
GGCAGTC13300.017.5263168
CTCTATG20550.017.3746971
TAACGAA1502.5102054E-1017.26666613
CTTATAC36250.017.1986237
CTTATTG13150.017.16349828
GCCTTAG650.001580106817.0769231
GGGCAGT14250.016.8771937
CCGCTTA3200.016.76562525
GTATAGA2000.016.651
TCTATGG21500.016.5209312
TCGGTGA14200.016.41549313
Produced by FastQC (version 0.11.5)