FastQCFastQC Report
Fri 10 Feb 2017
ERR1631508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931479
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28360.14683048586083514No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC25930.13424945339814723No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21890.11333283975647676No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC21660.11214204244519356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC2600.022.0576938
GTCTAGA3000.020.9666671
GGTATCA12250.019.6326521
TAGAACT3900.019.4487194
ATACACA6650.018.91729237
ACAACGG2350.018.89361823
ACGGAGT2350.018.89361826
CTAGAAC4150.018.722893
CGAACAA609.239917E-418.522
AACGGAG2300.018.525
TATACAC10050.017.85572237
CTTATAC32750.017.62442637
TATAGAC752.068184E-417.2666663
GTATCAA12300.017.1463432
TAGATAA2800.016.5178574
ATTACAA2700.016.44444320
CAAGACG4800.015.8020844
CAACGGA2700.015.75925824
AAGACGG4350.015.7356315
GTCTTAT957.063654E-515.5789481
Produced by FastQC (version 0.11.5)