##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631508.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1931479
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.084658440500775	34.0	33.0	34.0	31.0	34.0
2	33.23523113634681	34.0	33.0	34.0	31.0	34.0
3	33.3237752002481	34.0	34.0	34.0	31.0	34.0
4	36.53126024150405	37.0	37.0	37.0	35.0	37.0
5	36.52063729401148	37.0	37.0	37.0	35.0	37.0
6	36.57122702343644	37.0	37.0	37.0	35.0	37.0
7	36.57752323478537	37.0	37.0	37.0	35.0	37.0
8	36.56748067154756	37.0	37.0	37.0	35.0	37.0
9	38.42560079607389	39.0	39.0	39.0	37.0	39.0
10	38.38533372612387	39.0	39.0	39.0	37.0	39.0
11	38.445035125931994	39.0	39.0	39.0	37.0	39.0
12	38.40424514064093	39.0	39.0	39.0	37.0	39.0
13	38.42771937981205	39.0	39.0	39.0	37.0	39.0
14	39.98904414699823	41.0	40.0	41.0	38.0	41.0
15	39.990877457119645	41.0	40.0	41.0	38.0	41.0
16	39.98007071265077	41.0	40.0	41.0	38.0	41.0
17	39.95695526588692	41.0	40.0	41.0	38.0	41.0
18	39.939563930024605	41.0	40.0	41.0	38.0	41.0
19	39.948637805536585	41.0	40.0	41.0	38.0	41.0
20	39.92403127344382	41.0	40.0	41.0	38.0	41.0
21	39.88779479352351	41.0	40.0	41.0	38.0	41.0
22	39.8503437003457	41.0	40.0	41.0	38.0	41.0
23	39.80077753887047	41.0	40.0	41.0	38.0	41.0
24	39.7748409379548	41.0	40.0	41.0	38.0	41.0
25	39.75173532821221	41.0	40.0	41.0	38.0	41.0
26	39.68241694577057	41.0	40.0	41.0	37.0	41.0
27	39.63106148190066	41.0	40.0	41.0	37.0	41.0
28	39.56704421844607	41.0	40.0	41.0	37.0	41.0
29	39.53439825128826	41.0	40.0	41.0	37.0	41.0
30	39.476114418018525	41.0	40.0	41.0	37.0	41.0
31	39.44462714841839	41.0	39.0	41.0	37.0	41.0
32	39.37697847090235	41.0	39.0	41.0	36.0	41.0
33	39.32214018376591	41.0	39.0	41.0	36.0	41.0
34	39.26996772939287	41.0	39.0	41.0	36.0	41.0
35	39.197215191053076	41.0	39.0	41.0	35.0	41.0
36	39.14317629132908	41.0	39.0	41.0	35.0	41.0
37	39.095459489852075	41.0	39.0	41.0	35.0	41.0
38	39.03135162225424	40.0	39.0	41.0	35.0	41.0
39	38.959640254954884	40.0	39.0	41.0	35.0	41.0
40	38.88795529229156	40.0	38.0	41.0	35.0	41.0
41	38.81699930467792	40.0	38.0	41.0	35.0	41.0
42	38.71018375037989	40.0	38.0	41.0	35.0	41.0
43	37.881433347191454	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	1.0
15	3.0
16	4.0
17	12.0
18	15.0
19	30.0
20	83.0
21	154.0
22	301.0
23	523.0
24	829.0
25	1268.0
26	1968.0
27	2732.0
28	3948.0
29	5530.0
30	7490.0
31	10089.0
32	13372.0
33	18594.0
34	28409.0
35	44245.0
36	75858.0
37	146088.0
38	386115.0
39	1183816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64996978998995	16.40400956986848	11.66147806939656	28.284542570745007
2	20.414356045289647	18.0985141438245	32.998028971580844	28.48910083930501
3	21.140638857580125	19.016877739804574	27.15483833891024	32.68764506370507
4	16.020055097673854	14.07962499203978	30.614932908926267	39.2853870013601
5	17.79429131768971	32.98037410709617	31.695089617852435	17.530244957361692
6	38.91748240596973	30.824875652285115	14.177736335730287	16.079905606014872
7	33.264508700327575	26.955923414129796	18.63970563490465	21.139862250637982
8	29.186493873347835	31.1794743820668	18.14578361970283	21.48824812488254
9	27.9551059058887	13.42660210129129	17.52216824516342	41.09612374765659
10	19.276057363295173	24.103653210829627	29.931104609472843	26.68918481640235
11	39.90449805563509	19.426097824516862	18.642397872304073	22.02700624754398
12	24.94627174305286	22.535114282888916	25.94861243637648	26.570001537681748
13	32.97504140609347	16.919883674634825	22.48753416423373	27.61754075503798
14	25.093154002709838	18.027014531351362	21.53443035104187	35.34540111489692
15	28.056841415309204	24.858049194425618	19.454832281376085	27.630277108889096
16	28.68972429935816	22.747697489851042	20.764450454806912	27.798127755983888
17	26.98274224053174	23.62091433559464	21.992524899312908	27.403818524560712
18	27.738587890419726	21.62653593437982	23.202219646188233	27.432656529012224
19	27.778919677614926	22.46439127735792	22.075104104160594	27.68158494086656
20	27.740555294673147	22.142720681923024	22.62602906891558	27.490694954488244
21	29.038679685360286	22.051029288954215	22.47774891676275	26.432542108922746
22	28.937979651862637	21.739972321728583	21.30559017209092	28.016457854317856
23	27.568873386663796	21.79604334295118	22.434569570779697	28.200513699605327
24	27.20070992229271	22.336975965050616	22.16845225860597	28.293861854050707
25	28.161890447682836	21.933140355137176	22.199568310087763	27.705400887092225
26	28.06812810286832	22.372648110592973	22.040674529725663	27.518549256813042
27	28.280555988442018	21.61897696014298	22.40376416207476	27.69670288934024
28	27.075417335627254	22.14473985997259	22.55121593348931	28.22862687091084
29	26.965294471231633	22.23135742091941	22.58849306671209	28.21485504113687
30	26.151513943459907	22.712698403658543	23.753092837147076	27.38269481573447
31	28.11519048356208	22.147017907002873	21.97217779742881	27.76561381200624
32	26.804019096246968	21.677274254599713	23.034265451501152	28.48444119765216
33	26.856724820720284	21.447087956949055	22.974052526587137	28.722134695743524
34	27.53221753899473	20.94762614555996	23.250679919377845	28.26947639606747
35	26.634770556656324	20.949282907036526	24.345022648447124	28.070923887860026
36	26.06028851465639	20.97879397083789	24.753414352421125	28.207503162084603
37	26.993821832906285	19.973502171134143	24.93715955493174	28.095516441027833
38	25.498232183730707	19.608393360735477	26.369429851424737	28.52394460410908
39	25.865619041159647	19.479165965563176	26.838914634847182	27.81630035842999
40	25.10387117851139	20.412181545851652	26.609712039323234	27.874235236313726
41	24.459183868941885	19.82558443555431	28.263056445345768	27.45217525015804
42	24.39922981300858	20.001252925866655	28.046383108488364	27.5531341526364
43	22.652537252540668	19.908267187994277	28.095205798250976	29.343989761214072
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	27.0
2	29.0
3	57.5
4	86.0
5	86.0
6	119.5
7	153.0
8	147.0
9	141.0
10	189.5
11	238.0
12	238.0
13	421.5
14	605.0
15	929.0
16	1253.0
17	1264.5
18	1276.0
19	1276.0
20	1613.5
21	1951.0
22	2115.0
23	2279.0
24	3012.5
25	3746.0
26	3746.0
27	4905.0
28	6064.0
29	7992.0
30	9920.0
31	12673.5
32	15427.0
33	15427.0
34	20595.5
35	25764.0
36	33098.0
37	40432.0
38	49009.5
39	57587.0
40	57587.0
41	65160.0
42	72733.0
43	81214.5
44	89696.0
45	103013.5
46	116331.0
47	116331.0
48	128228.5
49	140126.0
50	147907.5
51	155689.0
52	165676.5
53	175664.0
54	175664.0
55	174802.5
56	173941.0
57	168831.0
58	163721.0
59	160471.5
60	157222.0
61	157222.0
62	148017.5
63	138813.0
64	129356.5
65	119900.0
66	109753.5
67	99607.0
68	99607.0
69	81964.5
70	64322.0
71	53973.0
72	43624.0
73	32804.0
74	21984.0
75	21984.0
76	17046.5
77	12109.0
78	9655.0
79	7201.0
80	6175.0
81	5149.0
82	5149.0
83	4018.5
84	2888.0
85	2411.0
86	1934.0
87	1520.0
88	1106.0
89	1106.0
90	837.0
91	568.0
92	338.5
93	109.0
94	79.0
95	49.0
96	49.0
97	30.5
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1931479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.05103519063355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9801944078723	38.67374887993174
2	7.901801335478178	7.277722627390015
3	2.624625884738694	3.626002169410479
4	1.3128982867730359	2.418413008236303
5	0.8170143513097453	1.881217832170886
6	0.538274333538362	1.4872854165593958
7	0.37934988945553405	1.222861857621582
8	0.29465112908931695	1.0855191611721635
9	0.23473938862066546	0.9728992661398264
>10	1.5269359273631034	13.82967643279826
>50	0.20298374584416545	6.560470578913129
>100	0.1703312603457681	15.038739744238033
>500	0.013275048815287181	4.060472857119455
>1k	0.0029250107559107346	1.8649701682987927
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2836	0.14683048586083514	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2593	0.13424945339814723	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2189	0.11333283975647676	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	2166	0.11214204244519356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	5.177379614274864E-5	0.0	5.177379614274864E-5	0.0
4	0.0	5.177379614274864E-5	0.0	5.177379614274864E-5	0.0
5	0.0	1.0354759228549728E-4	0.0	5.177379614274864E-5	0.0
6	0.0	1.0354759228549728E-4	0.0	1.0354759228549728E-4	0.0
7	0.0	1.0354759228549728E-4	0.0	1.553213884282459E-4	0.0
8	0.0	1.0354759228549728E-4	0.0	1.553213884282459E-4	0.0
9	0.0	1.0354759228549728E-4	0.0	1.553213884282459E-4	0.0
10	0.0	1.0354759228549728E-4	0.0	2.588689807137432E-4	0.0
11	0.0	2.0709518457099455E-4	0.0	3.106427768564918E-4	0.0
12	0.0	2.0709518457099455E-4	0.0	3.106427768564918E-4	0.0
13	0.0	2.0709518457099455E-4	0.0	3.6241657299924046E-4	0.0
14	0.0	2.0709518457099455E-4	0.0	3.6241657299924046E-4	0.0
15	0.0	2.0709518457099455E-4	0.0	4.141903691419891E-4	0.0
16	0.0	2.0709518457099455E-4	0.0	6.212855537129836E-4	0.0
17	0.0	2.0709518457099455E-4	0.0	7.248331459984809E-4	0.0
18	0.0	2.588689807137432E-4	0.0	7.766069421412296E-4	0.0
19	0.0	2.588689807137432E-4	0.0	9.319283305694755E-4	0.0
20	0.0	2.588689807137432E-4	0.0	0.0012425711074259673	0.0
21	0.0	2.588689807137432E-4	0.0	0.001604987680425208	0.0
22	0.0	2.588689807137432E-4	0.0	0.0023815946225664375	0.0
23	0.0	3.6241657299924046E-4	0.0	0.0037794871184206507	0.0
24	0.0	3.6241657299924046E-4	0.0	0.00647172451784358	0.0
25	0.0	3.6241657299924046E-4	0.0	0.008180259790554286	0.0
26	0.0	3.6241657299924046E-4	0.0	0.014548436716112367	0.0
27	0.0	3.6241657299924046E-4	0.0	0.051204284385178406	5.177379614274864E-5
28	0.0	3.6241657299924046E-4	0.0	0.22495714424024285	5.177379614274864E-5
29	0.0	3.6241657299924046E-4	0.0	0.47823455497056916	5.177379614274864E-5
30	0.0	3.6241657299924046E-4	0.0	0.8085513743613055	5.177379614274864E-5
31	0.0	3.6241657299924046E-4	0.0	1.630512161923583	5.177379614274864E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGATC	260	0.0	22.057693	8
GTCTAGA	300	0.0	20.966667	1
GGTATCA	1225	0.0	19.632652	1
TAGAACT	390	0.0	19.448719	4
ATACACA	665	0.0	18.917292	37
ACAACGG	235	0.0	18.893618	23
ACGGAGT	235	0.0	18.893618	26
CTAGAAC	415	0.0	18.72289	3
CGAACAA	60	9.239917E-4	18.5	22
AACGGAG	230	0.0	18.5	25
TATACAC	1005	0.0	17.855722	37
CTTATAC	3275	0.0	17.624426	37
TATAGAC	75	2.068184E-4	17.266666	3
GTATCAA	1230	0.0	17.146343	2
TAGATAA	280	0.0	16.517857	4
ATTACAA	270	0.0	16.444443	20
CAAGACG	480	0.0	15.802084	4
CAACGGA	270	0.0	15.759258	24
AAGACGG	435	0.0	15.735631	5
GTCTTAT	95	7.063654E-5	15.578948	1
>>END_MODULE