FastQCFastQC Report
Fri 10 Feb 2017
ERR1631507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25160
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7663.044515103338633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5622.233704292527822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5142.042925278219396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2521.001589825119237No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1250.4968203497615262No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1050.417329093799682No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.37360890302066774No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.37360890302066774No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.3259141494435612No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA790.3139904610492846No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA720.2861685214626391No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT670.26629570747217807No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.22655007949125594No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC560.22257551669316375No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA540.21462639109697934No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA540.21462639109697934No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC530.21065182829888715No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.2066772655007949No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA510.20270270270270271No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT500.19872813990461047No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT490.19475357710651828No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.19475357710651828No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT470.18680445151033387No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA450.17885532591414946No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.17885532591414946No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1589825119236884No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG400.1589825119236884No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT380.151033386327504No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.151033386327504No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC370.14705882352941177No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT360.14308426073131955No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT360.14308426073131955No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA320.1271860095389507No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT300.11923688394276628No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT300.11923688394276628No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11923688394276628No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1152623211446741No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA290.1152623211446741No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT280.11128775834658187No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA280.11128775834658187No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10731319554848967No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT260.10333863275039745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAGT901.2369128E-1024.6666687
AGTCGGT901.2369128E-1024.66666811
GGCAGTC901.2369128E-1024.6666688
TGGGCAG1001.8189894E-1124.056
CAGTCGG952.4374458E-1023.36842210
GGTGATT1004.638423E-1022.19999915
GCCTTAT502.6017032E-422.19999926
CGCCTTA502.6017032E-422.19999925
TCGGTGA1004.638423E-1022.19999913
CGGTGAT1004.638423E-1022.19999914
CTCGCCT603.543356E-521.58333223
GCAGTCG1058.54925E-1021.1428579
GTGATTC1058.54925E-1021.14285716
GTCGGTG1058.54925E-1021.14285712
GATTCCT1001.1463271E-820.34999818
ATTCCTC1001.1463271E-820.34999819
TGATTCC1101.5261321E-920.18181817
TCGCCTT554.9535226E-420.18181824
TCCTCGC656.565631E-519.92307721
CCTCGCC656.565631E-519.92307722
Produced by FastQC (version 0.11.5)