FastQCFastQC Report
Fri 10 Feb 2017
ERR1631506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1508091
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31280.20741453930830434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT29790.197534498912864No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA25050.16610403483609412No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23690.15708601138790695No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21350.14156970633734967No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC21100.13991198143878586No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC18760.12439567638822856No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT17750.11769846779803075No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16130.10695641045533726No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT15330.10165169077993304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.022.8937511
ACTAGAC502.7026437E-422.23
CCGCTTA1650.020.18181825
AGTCGGT5350.019.71028111
CAGTCGG5500.019.17272810
CGGTGAT5750.018.6608714
TAGCGAA609.2386367E-418.510
GTATGGA609.2386367E-418.51
CGAATTA609.2386367E-418.515
TCGGTGA5700.018.513
GTCGGTG5850.018.0256412
GTGATTC5750.018.01739116
CGCTTAT2000.017.57526
CGCCTTA3700.017.525
ACTAGGG752.0678059E-417.2666662
ATACACA4650.017.10752937
CTCTATG9100.017.0769231
GGTGATT6300.017.03174615
TATACAC6450.016.92248237
GCAGTCG6300.016.7380969
Produced by FastQC (version 0.11.5)