FastQCFastQC Report
Fri 10 Feb 2017
ERR1631505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76217
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG1970.25847251925423465No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA1810.23747982733510897No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG1550.20336670296652978No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC1450.19024627051707624No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG1330.174501751577732No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC1310.1718776650878413No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1170.15350905965860637No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1120.14694884343387957No Hit
TCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCT1050.1377645407192621No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC980.12858023800464463No Hit
GGGGAGAGATGCCCCTGCGCCTAGTGGCCTACAGCATTCTGGG950.12464410826980858No Hit
CCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCCCTCTCTGT940.12333206502486323No Hit
ACGCTGGACGCTCCAAGCAGTGAGACTGCCCACCCACTCCCAC940.12333206502486323No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC910.11939593529002716No Hit
ATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGA880.11545980555519109No Hit
GCCACAGAGAGGGCGACAAGCAGGAGGATCAGGATCTTCTCCA880.11545980555519109No Hit
GCTTGGAGCGTCCAGCGTCATGGCAGATGCCAAAGTTGGTGTT870.11414776231024576No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC870.11414776231024576No Hit
GTCTCACTGCTTGGAGCGTCCAGCGTCATGGCAGATGCCAAAG850.11152367582035504No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC820.10758754608551897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAC502.6648195E-729.61
CACTATT502.6648195E-729.614
TCTTACA502.6648195E-729.62
TATGGGC250.005464593429.64
GGCACTA453.9440765E-628.77777712
GCACTAT453.9440765E-628.77777713
TATTCAT508.946532E-625.90000217
ACTATTC508.946532E-625.90000215
CTATTCA508.946532E-625.90000216
ACACTGG551.8726172E-523.5454546
CTTACAC551.8726172E-523.5454543
ACTGGGC551.8726172E-523.5454548
GGGCACT603.6678655E-521.58333211
TTACACT603.6678655E-521.5833324
CACTGGG603.6678655E-521.5833327
TTGTAGT450.003793608620.5555552
ATCTAAG450.003793608620.55555526
CACAACA450.003793608620.5555554
CGGGTTA450.003793608620.5555559
TCATGCA656.794683E-519.92307720
Produced by FastQC (version 0.11.5)