##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76217
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.06833121219675	34.0	33.0	34.0	31.0	34.0
2	33.21211803141031	34.0	33.0	34.0	31.0	34.0
3	33.290420772268654	34.0	34.0	34.0	31.0	34.0
4	36.513231956125274	37.0	37.0	37.0	35.0	37.0
5	36.498300903997794	37.0	37.0	37.0	35.0	37.0
6	36.55633257672173	37.0	37.0	37.0	35.0	37.0
7	36.57291680333784	37.0	37.0	37.0	35.0	37.0
8	36.561777556188254	37.0	37.0	37.0	35.0	37.0
9	38.42409173806369	39.0	39.0	39.0	37.0	39.0
10	38.369878111182544	39.0	39.0	39.0	37.0	39.0
11	38.42787042260913	39.0	39.0	39.0	37.0	39.0
12	38.39711612894761	39.0	39.0	39.0	37.0	39.0
13	38.42678142671583	39.0	39.0	39.0	37.0	39.0
14	39.991327394150915	41.0	40.0	41.0	38.0	41.0
15	39.98815224949814	41.0	40.0	41.0	38.0	41.0
16	39.96948187412257	41.0	40.0	41.0	38.0	41.0
17	39.98287783565346	41.0	40.0	41.0	38.0	41.0
18	39.944658015928205	41.0	40.0	41.0	38.0	41.0
19	39.94095805397746	41.0	40.0	41.0	38.0	41.0
20	39.91845651232665	41.0	40.0	41.0	38.0	41.0
21	39.89840849154388	41.0	40.0	41.0	38.0	41.0
22	39.86138263117152	41.0	40.0	41.0	38.0	41.0
23	39.81211540732382	41.0	40.0	41.0	38.0	41.0
24	39.79335318892111	41.0	40.0	41.0	38.0	41.0
25	39.77520763084351	41.0	40.0	41.0	38.0	41.0
26	39.696642481336184	41.0	40.0	41.0	38.0	41.0
27	39.656362753716365	41.0	40.0	41.0	37.0	41.0
28	39.59897398218245	41.0	40.0	41.0	37.0	41.0
29	39.57968694648176	41.0	40.0	41.0	37.0	41.0
30	39.53203353582534	41.0	40.0	41.0	37.0	41.0
31	39.485127989818544	41.0	40.0	41.0	37.0	41.0
32	39.42834275817731	41.0	39.0	41.0	36.0	41.0
33	39.38555702795964	41.0	39.0	41.0	36.0	41.0
34	39.33782489470853	41.0	39.0	41.0	36.0	41.0
35	39.28426728944986	41.0	39.0	41.0	36.0	41.0
36	39.21046485692168	41.0	39.0	41.0	35.0	41.0
37	39.15059632365483	41.0	39.0	41.0	35.0	41.0
38	39.09169870238923	41.0	39.0	41.0	35.0	41.0
39	39.01838172586169	40.0	39.0	41.0	35.0	41.0
40	38.96533581746854	40.0	39.0	41.0	35.0	41.0
41	38.88539302255403	40.0	38.0	41.0	35.0	41.0
42	38.781387354527205	40.0	38.0	41.0	35.0	41.0
43	37.98394059068187	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	3.0
21	3.0
22	14.0
23	23.0
24	30.0
25	50.0
26	82.0
27	112.0
28	165.0
29	270.0
30	278.0
31	424.0
32	555.0
33	730.0
34	1128.0
35	1692.0
36	2740.0
37	5479.0
38	14604.0
39	47832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42447223060472	15.77338389073304	11.174672317199574	28.627471561462663
2	21.45453114134642	17.54333022816432	32.169988322815115	28.83215030767414
3	22.18927535851582	18.909167246152432	26.29990684492961	32.60165055040214
4	16.534368972801346	13.5678391959799	30.02479761732947	39.87299421388929
5	18.09701247753126	32.777464345224814	31.17283545665665	17.95268772058727
6	38.70396368264298	30.145505595864442	14.042798850650119	17.107731870842464
7	33.70376687615624	26.48096881273207	18.005169450385083	21.810094860726608
8	29.90540168203944	30.208483671621817	17.755881233845468	22.130233412493276
9	28.98565936733275	13.16110579004684	16.94503850846924	40.90819633415118
10	19.03118726793235	24.45517404253644	29.636432816825643	26.877205872705563
11	40.144587165592974	19.209625149244918	18.24920949394492	22.39657819121718
12	25.58615531967934	22.119737066533713	25.667502000865948	26.626605612921
13	33.92419014130706	16.552737578230577	21.918994450057074	27.604077830405288
14	24.997047902698874	17.556450660613773	20.97432331369642	36.47217812299093
15	27.978010155214715	25.272576984137395	18.402718553603528	28.346694307044363
16	28.311269139430834	22.69441200781978	20.28812469659	28.706194156159388
17	28.123646955403654	23.088024981303384	21.672330320007347	27.115997743285618
18	28.91218494561581	21.0373013894538	22.235196872088906	27.815316792841493
19	28.147263733812665	22.455620137239723	21.713003660600652	27.684112468346957
20	27.707729246755974	21.392865108833988	22.540902948161172	28.358502696248866
21	28.523820145111983	21.113399897660624	22.836112678273874	27.526667278953514
22	29.783381660259522	21.295773908708032	21.07666268680216	27.84418174423029
23	27.954393376805697	21.202618838316912	22.029206082632484	28.813781702244906
24	28.134143301363213	21.709067530865816	21.642153325373602	28.514635842397368
25	28.031803928257474	21.966228006875106	21.639529238883714	28.362438825983705
26	28.04492436070693	21.602792028025245	21.77073356337825	28.581550047889582
27	29.06700604851936	21.312830470892322	21.90324993111773	27.71691354947059
28	27.89010325780338	22.20501987745516	21.97016413660994	27.934712728131515
29	27.681488381857065	21.56999094690161	22.160410407127017	28.588110264114302
30	26.808979623968405	21.75105291470407	23.412099662804884	28.02786779852264
31	28.09871813374969	21.482084049490272	22.32572785599013	28.093469960769905
32	27.1212459162654	21.17375388692811	23.081464765078657	28.62353543172783
33	27.64081504126376	21.713003660600652	21.601479984780298	29.044701313355286
34	27.928152511906788	21.45453114134642	22.57895220226459	28.038364144482202
35	26.77355445635488	20.945458362307622	23.17986800844956	29.10111917288794
36	26.230368552947503	20.828686513507485	24.5234002912736	28.41754464227141
37	27.60538987365024	19.549444349685764	25.200414605665404	27.644751170998592
38	25.79870632536048	18.982641667869373	26.17788682314969	29.040765183620454
39	27.110749570305835	19.490402403663225	25.67275017384573	27.726097852185205
40	25.83281944972906	19.419552068436175	25.342115276119504	29.405513205715263
41	24.556201372397236	20.033588307070602	27.373158219294908	28.037052101237258
42	24.82254615112114	19.41561593870134	26.91919125654382	28.842646653633704
43	23.47245365207237	19.764619441856805	27.04777149454846	29.715155411522364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	6.5
4	10.0
5	10.0
6	13.0
7	16.0
8	14.5
9	13.0
10	16.5
11	20.0
12	20.0
13	35.5
14	51.0
15	85.0
16	119.0
17	110.5
18	102.0
19	102.0
20	116.5
21	131.0
22	110.0
23	89.0
24	99.0
25	109.0
26	109.0
27	135.0
28	161.0
29	205.5
30	250.0
31	350.0
32	450.0
33	450.0
34	557.0
35	664.0
36	918.0
37	1172.0
38	1465.0
39	1758.0
40	1758.0
41	2053.5
42	2349.0
43	2512.0
44	2675.0
45	3332.0
46	3989.0
47	3989.0
48	4645.5
49	5302.0
50	5553.5
51	5805.0
52	6321.5
53	6838.0
54	6838.0
55	7107.0
56	7376.0
57	7393.5
58	7411.0
59	7189.5
60	6968.0
61	6968.0
62	6828.0
63	6688.0
64	6095.5
65	5503.0
66	4852.5
67	4202.0
68	4202.0
69	3407.0
70	2612.0
71	2199.5
72	1787.0
73	1259.0
74	731.0
75	731.0
76	524.5
77	318.0
78	255.0
79	192.0
80	156.0
81	120.0
82	120.0
83	106.5
84	93.0
85	80.0
86	67.0
87	56.5
88	46.0
89	46.0
90	33.0
91	20.0
92	11.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	76217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.4670742747681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09619395843048	42.82509151501633
2	8.696719098917818	9.299762520172665
3	3.3937817476871732	5.443667423278272
4	1.916517386076415	4.098823097209284
5	1.288311943265196	3.444113517981553
6	0.9202228166179971	2.9520973011270453
7	0.6429290078771073	2.4062873112297782
8	0.47115408210841453	2.0152984242360628
9	0.36072734411425483	1.7358332130627028
>10	2.07356874677922	19.836781820328795
>50	0.11778852052710363	4.268076675807234
>100	0.02208534759883193	1.674167180550271
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAAGAGCAATTGCTGCCAGCATTCAAGTGCGCTGGGCCTG	197	0.25847251925423465	No Hit
CTCTTACACTGGGCACTATTCATGCAGAGCTCACCGTTCTCCA	181	0.23747982733510897	No Hit
CTCCCACACCTAGCCCAGAATGCTGTAGGCCACTAGGCGCAGG	155	0.20336670296652978	No Hit
GTGTGGGAGTGGGTGGGCAGTCTCACTGCTTGGAGCGTCCAGC	145	0.19024627051707624	No Hit
GGTCAAGGAGGTGGCCAGCCCGGGAGATGCGCTGGAGCAGGGG	133	0.174501751577732	No Hit
CTCTGTGGCCTATGCAGCTCCTGGCCCCCGGGGGATCATTATC	131	0.1718776650878413	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	117	0.15350905965860637	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	112	0.14694884343387957	No Hit
TCTCTCCCCTGCTCCAGCGCATCTCCCGGGCTGGCCACCTCCT	105	0.1377645407192621	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	98	0.12858023800464463	No Hit
GGGGAGAGATGCCCCTGCGCCTAGTGGCCTACAGCATTCTGGG	95	0.12464410826980858	No Hit
CCATGGAGAAGATCCTGATCCTCCTGCTTGTCGCCCTCTCTGT	94	0.12333206502486323	No Hit
ACGCTGGACGCTCCAAGCAGTGAGACTGCCCACCCACTCCCAC	94	0.12333206502486323	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	91	0.11939593529002716	No Hit
ATTGTGAAGAGCGCAATCAGAAAACAGATATGCTGGTCAAGGA	88	0.11545980555519109	No Hit
GCCACAGAGAGGGCGACAAGCAGGAGGATCAGGATCTTCTCCA	88	0.11545980555519109	No Hit
GCTTGGAGCGTCCAGCGTCATGGCAGATGCCAAAGTTGGTGTT	87	0.11414776231024576	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	87	0.11414776231024576	No Hit
GTCTCACTGCTTGGAGCGTCCAGCGTCATGGCAGATGCCAAAG	85	0.11152367582035504	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	82	0.10758754608551897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0013120432449453534	0.0
18	0.0	0.0	0.0	0.0013120432449453534	0.0
19	0.0	0.0	0.0	0.0013120432449453534	0.0
20	0.0	0.0	0.0	0.0013120432449453534	0.0
21	0.0	0.0	0.0	0.0013120432449453534	0.0
22	0.0	0.0	0.0	0.002624086489890707	0.0
23	0.0	0.0	0.0	0.002624086489890707	0.0
24	0.0	0.0	0.0	0.006560216224726767	0.0
25	0.0	0.0	0.0	0.010496345959562827	0.0
26	0.0	0.0	0.0	0.017056562184289595	0.0
27	0.0	0.0	0.0	0.04985764330792343	0.0
28	0.0	0.0	0.0	0.17843788131256808	0.0
29	0.0	0.0	0.0	0.3647480220948082	0.0
30	0.0	0.0	0.0	0.5851712872456276	0.0
31	0.0	0.0	0.0	1.26218560163743	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAC	50	2.6648195E-7	29.6	1
CACTATT	50	2.6648195E-7	29.6	14
TCTTACA	50	2.6648195E-7	29.6	2
TATGGGC	25	0.0054645934	29.6	4
GGCACTA	45	3.9440765E-6	28.777777	12
GCACTAT	45	3.9440765E-6	28.777777	13
TATTCAT	50	8.946532E-6	25.900002	17
ACTATTC	50	8.946532E-6	25.900002	15
CTATTCA	50	8.946532E-6	25.900002	16
ACACTGG	55	1.8726172E-5	23.545454	6
CTTACAC	55	1.8726172E-5	23.545454	3
ACTGGGC	55	1.8726172E-5	23.545454	8
GGGCACT	60	3.6678655E-5	21.583332	11
TTACACT	60	3.6678655E-5	21.583332	4
CACTGGG	60	3.6678655E-5	21.583332	7
TTGTAGT	45	0.0037936086	20.555555	2
ATCTAAG	45	0.0037936086	20.555555	26
CACAACA	45	0.0037936086	20.555555	4
CGGGTTA	45	0.0037936086	20.555555	9
TCATGCA	65	6.794683E-5	19.923077	20
>>END_MODULE