FastQCFastQC Report
Fri 10 Feb 2017
ERR1631504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459813
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3600.023.638891
TACGCTA400.001929435323.1259
TATTAGA502.6986867E-422.22
TAACGCC705.090944E-621.1428594
ATACACA3700.021.037
TATTACC450.003822370220.5555552
ATTAGAG759.2459595E-619.7333343
TCGTTTA801.6132359E-518.530
TAGCGCC500.007028620718.529
TAACGAA801.6132359E-518.513
ATATACG500.007028620718.56
AACGCCG801.6132359E-518.55
ACGCTAT500.007028620718.510
ATAACGA801.6132359E-518.512
CGGTCCA500.007028620718.510
ATACGCT500.007028620718.58
GAATAAC1156.380105E-817.6956521
AATAACG953.596875E-617.5263162
CGAACGA852.7173659E-517.41176416
ATCGTTT852.7173659E-517.41176429
Produced by FastQC (version 0.11.5)