##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631504.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459813
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.08524987331807	34.0	33.0	34.0	31.0	34.0
2	33.22858205400891	34.0	33.0	34.0	31.0	34.0
3	33.3125031262709	34.0	34.0	34.0	31.0	34.0
4	36.53595700861002	37.0	37.0	37.0	35.0	37.0
5	36.530761418228714	37.0	37.0	37.0	35.0	37.0
6	36.58069693549334	37.0	37.0	37.0	35.0	37.0
7	36.586953826881796	37.0	37.0	37.0	35.0	37.0
8	36.581340675448494	37.0	37.0	37.0	35.0	37.0
9	38.431279672388555	39.0	39.0	39.0	37.0	39.0
10	38.39150480738909	39.0	39.0	39.0	37.0	39.0
11	38.446098740140016	39.0	39.0	39.0	37.0	39.0
12	38.40661747275523	39.0	39.0	39.0	37.0	39.0
13	38.43183424566074	39.0	39.0	39.0	37.0	39.0
14	39.997575101182434	41.0	40.0	41.0	38.0	41.0
15	40.00076552859532	41.0	40.0	41.0	38.0	41.0
16	39.98478294437086	41.0	40.0	41.0	38.0	41.0
17	39.96510755459285	41.0	40.0	41.0	38.0	41.0
18	39.94461444108801	41.0	40.0	41.0	38.0	41.0
19	39.962723976921055	41.0	40.0	41.0	38.0	41.0
20	39.94142836326942	41.0	40.0	41.0	38.0	41.0
21	39.90894124350551	41.0	40.0	41.0	38.0	41.0
22	39.8660303210218	41.0	40.0	41.0	38.0	41.0
23	39.82180799585919	41.0	40.0	41.0	38.0	41.0
24	39.804701041510356	41.0	40.0	41.0	38.0	41.0
25	39.77766178859667	41.0	40.0	41.0	38.0	41.0
26	39.69620258670373	41.0	40.0	41.0	37.0	41.0
27	39.65850465297849	41.0	40.0	41.0	37.0	41.0
28	39.60359102504714	41.0	40.0	41.0	37.0	41.0
29	39.55892939086107	41.0	40.0	41.0	37.0	41.0
30	39.504894380976616	41.0	40.0	41.0	37.0	41.0
31	39.463218743271725	41.0	39.0	41.0	36.0	41.0
32	39.40951647735058	41.0	39.0	41.0	36.0	41.0
33	39.347223762703535	41.0	39.0	41.0	36.0	41.0
34	39.29826907895166	41.0	39.0	41.0	35.0	41.0
35	39.23893843801719	41.0	39.0	41.0	35.0	41.0
36	39.19444426321135	41.0	39.0	41.0	35.0	41.0
37	39.154849906374984	41.0	39.0	41.0	35.0	41.0
38	39.11049056899218	41.0	39.0	41.0	35.0	41.0
39	39.05339561952359	41.0	39.0	41.0	35.0	41.0
40	38.97313038126369	41.0	39.0	41.0	35.0	41.0
41	38.909084780117134	40.0	39.0	41.0	35.0	41.0
42	38.81583600289683	40.0	38.0	41.0	35.0	41.0
43	38.0269783585936	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	0.0
17	2.0
18	2.0
19	16.0
20	14.0
21	37.0
22	79.0
23	125.0
24	189.0
25	295.0
26	444.0
27	674.0
28	933.0
29	1284.0
30	1800.0
31	2416.0
32	3037.0
33	4412.0
34	6850.0
35	10591.0
36	17573.0
37	33462.0
38	85585.0
39	289991.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.998103576888866	17.064980763919245	12.229319310241337	26.707596348950553
2	20.068375622263833	19.490532020625775	32.43557707154865	28.00551528556174
3	19.918532098918472	20.67057695193481	28.858470726143022	30.5524202230037
4	15.093092191825807	15.527181702126732	32.91164016676345	36.46808593928401
5	16.851415684202056	33.5471158927651	32.448625854423426	17.152842568609415
6	35.480945514807104	33.33616056962287	15.844049646269246	15.33884426930078
7	31.217908149617347	29.167291920846083	19.86198737312777	19.7528125564088
8	28.76125729372593	31.73898954575012	19.111029918684334	20.388723241839617
9	27.661462377096775	14.63594983177944	18.473814354966038	39.228773436157745
10	18.735224971890744	24.416882515283387	30.59874340220916	26.24914911061671
11	37.711852426964874	20.801282260397162	20.18972930299926	21.297136009638702
12	24.343156892040895	23.87927266084256	26.77240530389528	25.005165143221262
13	31.386019968987394	17.987312233451426	23.468236000287074	27.15843179727411
14	24.473427241074088	18.963796151913932	22.962160704460292	33.60061590255169
15	26.87092361459985	25.328774958515744	20.713420455706995	27.08688097117741
16	27.002063882491363	24.240723946473892	22.1851056842673	26.57210648676745
17	26.088431601542368	24.474514639646987	23.12592292953875	26.3111308292719
18	26.173466169942998	23.19355912077301	24.124807258602953	26.508167450681036
19	26.724342286973187	23.798152727304362	23.506947389482246	25.970557596240212
20	26.85874475058339	23.173116027602525	23.191384323627215	26.776754898186873
21	28.09620432654144	23.428219732804422	22.794266364804823	25.681309575849316
22	27.971805929801896	23.16941887245467	22.533073227594695	26.325701970148735
23	26.85374271714806	23.0400184422798	23.152890414146622	26.953348426425528
24	26.28633814180982	23.599158788464006	23.149193258998768	26.965309810727405
25	26.42074060542003	22.7390265173016	23.502597795190653	27.33763508208772
26	26.25850073834363	23.762921013542464	23.644394569096566	26.334183679017343
27	26.796110592784455	23.148758299569607	23.26228271058017	26.792848397065764
28	26.216744633144344	23.409081517921415	23.774012478986023	26.600161369948218
29	25.743943733648244	23.60872789590551	24.16025645207943	26.487071918366816
30	25.627592086348145	23.79793524758978	23.861656803961612	26.712815862100463
31	26.13779949675194	23.15049813728624	23.454099818839396	27.257602547122417
32	25.363354233133904	22.591575270816612	24.26442923536307	27.780641260686412
33	25.20241924434498	22.25361179435988	25.183498509176555	27.36047045211858
34	26.116269005008558	21.516138082220383	25.526681498783198	26.84091141398786
35	25.400325784612438	21.208186806375632	26.20891536341948	27.182572045592444
36	24.376431288371577	21.35150593828361	26.426612557713682	27.845450215631136
37	24.220715812732568	20.13470693521062	27.290876943453103	28.353700308603713
38	23.303386376635718	19.9257089294996	28.771478840311172	27.99942585355351
39	23.359496142997262	19.44921087485565	29.724692429313652	27.466600552833437
40	22.620717552570284	20.264324845100074	30.01829004399615	27.096667558333497
41	21.459158397000518	20.384373647548024	31.180936598138807	26.975531357312647
42	21.214711197813024	21.076611579054962	30.788820672751747	26.91985655038026
43	20.006611383323218	20.505727328283456	30.87603003829818	28.611631250095147
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	20.0
2	20.0
3	45.0
4	70.0
5	70.0
6	89.5
7	109.0
8	117.0
9	125.0
10	163.5
11	202.0
12	202.0
13	315.0
14	428.0
15	611.0
16	794.0
17	810.5
18	827.0
19	827.0
20	989.5
21	1152.0
22	1268.5
23	1385.0
24	1781.0
25	2177.0
26	2177.0
27	2760.0
28	3343.0
29	4290.5
30	5238.0
31	6381.5
32	7525.0
33	7525.0
34	9212.5
35	10900.0
36	12993.0
37	15086.0
38	17249.5
39	19413.0
40	19413.0
41	21436.5
42	23460.0
43	25723.0
44	27986.0
45	29562.0
46	31138.0
47	31138.0
48	32479.5
49	33821.0
50	34875.5
51	35930.0
52	36752.5
53	37575.0
54	37575.0
55	36556.5
56	35538.0
57	34547.5
58	33557.0
59	32539.5
60	31522.0
61	31522.0
62	29184.0
63	26846.0
64	24593.5
65	22341.0
66	19739.0
67	17137.0
68	17137.0
69	14703.0
70	12269.0
71	10419.5
72	8570.0
73	6739.5
74	4909.0
75	4909.0
76	3995.5
77	3082.0
78	2558.5
79	2035.0
80	1750.0
81	1465.0
82	1465.0
83	1127.5
84	790.0
85	669.5
86	549.0
87	425.0
88	301.0
89	301.0
90	223.5
91	146.0
92	86.0
93	26.0
94	15.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	459813.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.25029953996948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.7623946534755	67.3913498998548
2	5.40023295282059	8.019378286650854
3	1.4049726906572917	3.129589293803423
4	0.6715014075639024	1.9943672261252352
5	0.38165293251759075	1.4168922279869434
6	0.2493252257914524	1.110748361873152
7	0.18428223202310565	0.9578107649326824
8	0.1385362355446618	0.8229085589064723
9	0.10131051031933148	0.6770102163981778
>10	0.6258557157229289	8.650326765786387
>50	0.052898455693670555	2.690492420622415
>100	0.027036987869936843	3.139125977059472
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1747971457962257E-4	0.0
2	0.0	0.0	0.0	2.1747971457962257E-4	0.0
3	0.0	0.0	0.0	2.1747971457962257E-4	0.0
4	0.0	0.0	0.0	2.1747971457962257E-4	0.0
5	0.0	0.0	0.0	2.1747971457962257E-4	0.0
6	0.0	0.0	0.0	2.1747971457962257E-4	0.0
7	0.0	0.0	0.0	2.1747971457962257E-4	0.0
8	0.0	0.0	0.0	2.1747971457962257E-4	0.0
9	0.0	0.0	0.0	2.1747971457962257E-4	0.0
10	0.0	0.0	0.0	6.524391437388678E-4	0.0
11	0.0	0.0	0.0	6.524391437388678E-4	0.0
12	0.0	0.0	0.0	6.524391437388678E-4	0.0
13	0.0	0.0	0.0	6.524391437388678E-4	0.0
14	0.0	0.0	0.0	6.524391437388678E-4	0.0
15	0.0	0.0	0.0	6.524391437388678E-4	2.1747971457962257E-4
16	0.0	0.0	0.0	0.001087398572898113	2.1747971457962257E-4
17	0.0	0.0	0.0	0.001087398572898113	2.1747971457962257E-4
18	0.0	0.0	0.0	0.001087398572898113	2.1747971457962257E-4
19	0.0	0.0	0.0	0.0017398377166369806	2.1747971457962257E-4
20	0.0	0.0	0.0	0.0019573174312166033	2.1747971457962257E-4
21	0.0	0.0	0.0	0.0023922768603758483	4.3495942915924515E-4
22	0.0	0.0	0.0	0.004349594291592452	4.3495942915924515E-4
23	0.0	0.0	0.0	0.007829269724866413	4.3495942915924515E-4
24	0.0	0.0	0.0	0.013918701733095845	4.3495942915924515E-4
25	0.0	0.0	0.0	0.020443093170484525	4.3495942915924515E-4
26	0.0	0.0	0.0	0.030664639755726784	4.3495942915924515E-4
27	0.0	0.0	0.0	0.12200611987916828	4.3495942915924515E-4
28	0.0	0.0	0.0	0.5115122886912723	4.3495942915924515E-4
29	0.0	0.0	0.0	1.065433121725571	4.3495942915924515E-4
30	0.0	0.0	0.0	1.8503174116434289	4.3495942915924515E-4
31	0.0	0.0	0.0	3.5481815433665425	4.3495942915924515E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	360	0.0	23.63889	1
TACGCTA	40	0.0019294353	23.125	9
TATTAGA	50	2.6986867E-4	22.2	2
TAACGCC	70	5.090944E-6	21.142859	4
ATACACA	370	0.0	21.0	37
TATTACC	45	0.0038223702	20.555555	2
ATTAGAG	75	9.2459595E-6	19.733334	3
TCGTTTA	80	1.6132359E-5	18.5	30
TAGCGCC	50	0.0070286207	18.5	29
TAACGAA	80	1.6132359E-5	18.5	13
ATATACG	50	0.0070286207	18.5	6
AACGCCG	80	1.6132359E-5	18.5	5
ACGCTAT	50	0.0070286207	18.5	10
ATAACGA	80	1.6132359E-5	18.5	12
CGGTCCA	50	0.0070286207	18.5	10
ATACGCT	50	0.0070286207	18.5	8
GAATAAC	115	6.380105E-8	17.695652	1
AATAACG	95	3.596875E-6	17.526316	2
CGAACGA	85	2.7173659E-5	17.411764	16
ATCGTTT	85	2.7173659E-5	17.411764	29
>>END_MODULE