FastQCFastQC Report
Fri 10 Feb 2017
ERR1631503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3974365
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT323020.8127587677528361No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230320.579513960091738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228980.5761423523003046No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98350.24746091513990287No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA43480.1094011244563597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCG36450.020.048019
GTACTAG759.275174E-619.7333341
CAGTCGG37750.019.40662210
AGTCGGT37550.019.31291811
GGCAGTC38400.019.0781258
TCGGTGA39000.018.59487213
GGGCAGT41100.018.094897
ATACACA19250.017.58701337
CGGTGAT41000.017.55243914
CTTATAC99750.017.21102737
GTGATTC41500.017.11807316
GTCGGTG42150.017.07354512
GGTATCA148500.016.9053881
CGCTTAT11750.016.53191626
CTCTATG62000.016.4709681
CGCCTTA29550.016.40270825
CCGCTTA12100.016.35950325
ATTCCTC45000.015.99222319
GATTCCT45000.015.86888918
TTCCTCG31700.015.75709720
Produced by FastQC (version 0.11.5)