##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631503.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3974365
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07666985795215	34.0	33.0	34.0	31.0	34.0
2	33.20660835127121	34.0	33.0	34.0	31.0	34.0
3	33.27108204706916	34.0	34.0	34.0	31.0	34.0
4	36.50605316824197	37.0	37.0	37.0	35.0	37.0
5	36.515031961080574	37.0	37.0	37.0	35.0	37.0
6	36.58132582186085	37.0	37.0	37.0	35.0	37.0
7	36.58011380434359	37.0	37.0	37.0	35.0	37.0
8	36.58518027408152	37.0	37.0	37.0	35.0	37.0
9	38.43039605068986	39.0	39.0	39.0	37.0	39.0
10	38.39681534031222	39.0	39.0	39.0	37.0	39.0
11	38.45237490768966	39.0	39.0	39.0	37.0	39.0
12	38.418332236722094	39.0	39.0	39.0	37.0	39.0
13	38.44188945907082	39.0	39.0	39.0	37.0	39.0
14	39.97253875776382	41.0	40.0	41.0	38.0	41.0
15	39.96178810954706	41.0	40.0	41.0	38.0	41.0
16	39.93151283286764	41.0	40.0	41.0	38.0	41.0
17	39.93232881227567	41.0	40.0	41.0	38.0	41.0
18	39.946170268709594	41.0	40.0	41.0	38.0	41.0
19	39.97422959390997	41.0	40.0	41.0	38.0	41.0
20	39.95873755933338	41.0	40.0	41.0	38.0	41.0
21	39.92749835508314	41.0	40.0	41.0	38.0	41.0
22	39.87412630696979	41.0	40.0	41.0	38.0	41.0
23	39.81267145820779	41.0	40.0	41.0	38.0	41.0
24	39.78521575144708	41.0	40.0	41.0	38.0	41.0
25	39.75864798527564	41.0	40.0	41.0	37.0	41.0
26	39.65747609995559	41.0	40.0	41.0	37.0	41.0
27	39.588912694229144	41.0	40.0	41.0	37.0	41.0
28	39.50547144009169	41.0	40.0	41.0	36.0	41.0
29	39.42690392050051	41.0	39.0	41.0	36.0	41.0
30	39.3177347827892	41.0	39.0	41.0	35.0	41.0
31	39.21760985717215	41.0	39.0	41.0	35.0	41.0
32	39.116850113162734	41.0	39.0	41.0	35.0	41.0
33	39.015582866696946	41.0	39.0	41.0	35.0	41.0
34	38.94009332308432	41.0	39.0	41.0	35.0	41.0
35	38.828383905353434	41.0	39.0	41.0	35.0	41.0
36	38.74046646445407	41.0	38.0	41.0	35.0	41.0
37	38.645460595592	40.0	38.0	41.0	35.0	41.0
38	38.542334184203014	40.0	38.0	41.0	35.0	41.0
39	38.419095377500554	40.0	38.0	41.0	35.0	41.0
40	38.28073893565387	40.0	38.0	41.0	35.0	41.0
41	38.14008376180849	40.0	37.0	41.0	35.0	41.0
42	37.962829282162055	40.0	37.0	41.0	34.0	41.0
43	37.15957090000541	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	12.0
11	9.0
12	6.0
13	11.0
14	13.0
15	23.0
16	24.0
17	36.0
18	46.0
19	114.0
20	189.0
21	401.0
22	743.0
23	1259.0
24	2091.0
25	3427.0
26	5202.0
27	7985.0
28	11985.0
29	17230.0
30	23135.0
31	29086.0
32	35663.0
33	46414.0
34	69179.0
35	102663.0
36	165288.0
37	371533.0
38	734752.0
39	2345843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.77168050745213	17.02470709157312	13.16620390930375	25.037408491671
2	19.8378608909851	20.455796083147874	33.316190133518184	26.39015289234884
3	19.57530322454027	22.719352651304046	29.405200579212025	28.30014354494366
4	14.05618255998128	16.3456300566254	33.499439533107804	36.09874785028552
5	15.382734097145079	35.57637509388292	32.2104537454411	16.830437063530905
6	32.76807741614069	34.21628360757001	17.07477798340112	15.940860992888172
7	29.867739877942768	30.345803669265404	20.402957453580633	19.383498999211195
8	29.35165743458389	31.229844264429662	19.26184434494567	20.156653956040778
9	28.05467036872557	14.277198999085389	19.119658108905448	38.54847252328359
10	19.19836250570846	25.345583508308874	29.35175807959259	26.104295906390078
11	35.841952110588736	22.480522045660127	19.994439363269354	21.683086480481787
12	23.771143314718202	26.3983051380535	26.57269777687756	23.257853770350735
13	31.074221919728057	18.702409064089483	24.76315084296485	25.46021817321761
14	23.954719810586095	20.120723687935055	24.643181992595043	31.281374508883808
15	26.845018009166242	26.02591860586534	21.892805517359378	25.23625786760904
16	25.326284827890746	25.07937746029869	24.072046729477538	25.52229098233303
17	23.725651770786023	26.455421180490468	24.647157470438675	25.17176957828483
18	24.33581213602676	23.642443509843712	26.75287750370185	25.268866850427678
19	24.801144333748915	24.93887702815418	26.73657301229253	23.523405625804376
20	25.464344618574287	23.53052626016986	26.72361496742247	24.28151415383338
21	27.18997877648379	23.688689891341134	25.75822804397684	23.36310328819824
22	26.664561508568035	23.538477215857124	24.990809852643125	24.806151422931713
23	24.78972112526152	24.03541194631092	25.8265660048838	25.34830092354376
24	24.101183459496045	24.91394222724888	26.103616552581354	24.881257760673716
25	24.356217911540586	23.84033675819911	26.15426615320938	25.649179177050925
26	24.822632043106257	24.549733102017555	26.148680355226556	24.47895449964963
27	24.901764181196242	24.155355635428553	25.567505752491275	25.37537443088393
28	23.84936964772989	24.411547505073138	27.16340849418712	24.575674353009852
29	23.9229663103414	24.55340664483509	27.429186800910333	24.09444024391318
30	23.469686352411014	24.985022764642906	27.412630696979267	24.132660185966813
31	24.353525657557874	24.778197271765425	26.04000890708327	24.828268163593428
32	23.33137494920572	24.270719976650355	27.158023986221696	25.23988108792222
33	23.069446314065264	23.821138722789676	28.01141817623696	25.0979967869081
34	24.322753446148003	23.470290222463213	27.100002138706436	25.106954192682352
35	23.754285275761035	23.66684992445334	27.70377154589475	24.875093253890874
36	22.416386013866365	23.851961256703902	27.712301210382034	26.019351519047696
37	22.839648597952124	22.7895022223676	28.51054193562997	25.860307244050308
38	22.16421994456976	21.988317630615207	29.824563169210677	26.022899255604354
39	22.23811854220737	21.869782971619365	30.399145523876143	25.49295296229712
40	21.70932966650018	22.33878871215905	30.501375691462663	25.45050592987811
41	20.485008296922906	22.590074137629536	31.56207343814672	25.36284412730084
42	20.591742328648728	22.95531487420003	31.061767099901495	25.39117569724975
43	19.479765950032267	22.729467474678344	31.34410151055577	26.446665064733608
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	218.0
1	383.5
2	549.0
3	2225.5
4	3902.0
5	3902.0
6	5325.0
7	6748.0
8	6786.0
9	6824.0
10	10367.0
11	13910.0
12	13910.0
13	22488.5
14	31067.0
15	46944.0
16	62821.0
17	54706.5
18	46592.0
19	46592.0
20	48522.5
21	50453.0
22	37340.5
23	24228.0
24	24686.0
25	25144.0
26	25144.0
27	27477.0
28	29810.0
29	37430.0
30	45050.0
31	54479.0
32	63908.0
33	63908.0
34	80158.0
35	96408.0
36	116430.5
37	136453.0
38	157553.0
39	178653.0
40	178653.0
41	197138.5
42	215624.0
43	237044.5
44	258465.0
45	277370.0
46	296275.0
47	296275.0
48	315170.0
49	334065.0
50	328586.5
51	323108.0
52	308329.5
53	293551.0
54	293551.0
55	277292.0
56	261033.0
57	246190.0
58	231347.0
59	224766.0
60	218185.0
61	218185.0
62	201822.5
63	185460.0
64	169615.5
65	153771.0
66	138031.5
67	122292.0
68	122292.0
69	105351.5
70	88411.0
71	77043.5
72	65676.0
73	51129.0
74	36582.0
75	36582.0
76	29981.5
77	23381.0
78	19364.0
79	15347.0
80	13499.5
81	11652.0
82	11652.0
83	9304.0
84	6956.0
85	6075.5
86	5195.0
87	4303.0
88	3411.0
89	3411.0
90	2474.0
91	1537.0
92	875.0
93	213.0
94	138.5
95	64.0
96	64.0
97	40.0
98	16.0
99	13.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3974365.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.34041972527449
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75962569146003	37.976965848826815
2	8.599944738032661	7.79850208073133
3	2.6507171451455545	3.6055388380164235
4	1.2652068952833244	2.2946004668582933
5	0.765779694895679	1.7360386391831357
6	0.49013288580364867	1.3333698458098475
7	0.33998250967538324	1.0790464781553848
8	0.25646198257256536	0.9302475146732914
9	0.20562416301023878	0.8390777270888236
>10	1.3299814773912333	11.689148356996833
>50	0.16692244330768358	5.342069922586578
>100	0.14581776041371525	13.117320121626182
>500	0.017001866438417532	5.203030539201472
>1k	0.006577771272497639	4.82919564414718
>5k	5.574382434320033E-5	0.24857454943312665
>10k+	1.6723147302960097E-4	1.9772734266652128
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32302	0.8127587677528361	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23032	0.579513960091738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22898	0.5761423523003046	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9835	0.24746091513990287	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	4348	0.1094011244563597	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.54837565246272E-5	0.0
2	0.0	0.0	0.0	7.54837565246272E-5	0.0
3	0.0	0.0	0.0	1.0064500869950293E-4	0.0
4	0.0	0.0	0.0	1.0064500869950293E-4	0.0
5	0.0	5.0322504349751467E-5	0.0	1.0064500869950293E-4	0.0
6	0.0	5.0322504349751467E-5	0.0	1.0064500869950293E-4	0.0
7	0.0	5.0322504349751467E-5	0.0	1.0064500869950293E-4	0.0
8	0.0	5.0322504349751467E-5	0.0	1.0064500869950293E-4	5.0322504349751467E-5
9	0.0	5.0322504349751467E-5	0.0	1.7612876522413013E-4	5.0322504349751467E-5
10	0.0	5.0322504349751467E-5	0.0	3.5225753044826027E-4	5.0322504349751467E-5
11	0.0	5.0322504349751467E-5	0.0	0.0013587076174432897	5.0322504349751467E-5
12	0.0	5.0322504349751467E-5	0.0	0.002264512695738816	5.0322504349751467E-5
13	0.0	5.0322504349751467E-5	0.0	0.002667092730536828	5.0322504349751467E-5
14	0.0	5.0322504349751467E-5	0.0	0.0034470915479579757	5.0322504349751467E-5
15	0.0	5.0322504349751467E-5	0.0	0.0051832179480244015	5.0322504349751467E-5
16	0.0	7.54837565246272E-5	0.0	0.009334824556878897	7.54837565246272E-5
17	0.0	7.54837565246272E-5	0.0	0.013109012383110259	7.54837565246272E-5
18	0.0	1.0064500869950293E-4	0.0	0.014794816278826933	7.54837565246272E-5
19	0.0	1.0064500869950293E-4	0.0	0.01741158650501401	7.54837565246272E-5
20	0.0	1.0064500869950293E-4	0.0	0.02017932424425034	7.54837565246272E-5
21	0.0	1.0064500869950293E-4	0.0	0.02508576841835111	1.0064500869950293E-4
22	0.0	1.0064500869950293E-4	0.0	0.034018012940431996	1.0064500869950293E-4
23	0.0	1.2580626087437867E-4	0.0	0.046699284036569366	1.0064500869950293E-4
24	0.0	1.2580626087437867E-4	0.0	0.06650118949819657	1.0064500869950293E-4
25	0.0	1.2580626087437867E-4	0.0	0.0749805314811297	1.0064500869950293E-4
26	0.0	1.2580626087437867E-4	0.0	0.08869341391643697	1.0064500869950293E-4
27	0.0	1.2580626087437867E-4	0.0	0.15084170678838002	1.0064500869950293E-4
28	0.0	1.509675130492544E-4	0.0	0.379255554031902	1.0064500869950293E-4
29	0.0	1.509675130492544E-4	0.0	0.7253485776973176	1.0064500869950293E-4
30	0.0	1.509675130492544E-4	0.0	1.2757761302748993	1.0064500869950293E-4
31	0.0	1.509675130492544E-4	0.0	2.4501272530328744	1.0064500869950293E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTCG	3645	0.0	20.04801	9
GTACTAG	75	9.275174E-6	19.733334	1
CAGTCGG	3775	0.0	19.406622	10
AGTCGGT	3755	0.0	19.312918	11
GGCAGTC	3840	0.0	19.078125	8
TCGGTGA	3900	0.0	18.594872	13
GGGCAGT	4110	0.0	18.09489	7
ATACACA	1925	0.0	17.587013	37
CGGTGAT	4100	0.0	17.552439	14
CTTATAC	9975	0.0	17.211027	37
GTGATTC	4150	0.0	17.118073	16
GTCGGTG	4215	0.0	17.073545	12
GGTATCA	14850	0.0	16.905388	1
CGCTTAT	1175	0.0	16.531916	26
CTCTATG	6200	0.0	16.470968	1
CGCCTTA	2955	0.0	16.402708	25
CCGCTTA	1210	0.0	16.359503	25
ATTCCTC	4500	0.0	15.992223	19
GATTCCT	4500	0.0	15.868889	18
TTCCTCG	3170	0.0	15.757097	20
>>END_MODULE