FastQCFastQC Report
Fri 10 Feb 2017
ERR1631502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280928
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24750.8810086570224399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22230.791305957398337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17070.6076290010251737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.37767684246497324No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4240.1509283517484907No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.1473687208110263No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT3660.13028249231119718No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3310.11782378403007177No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.10536507574894635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA502.6950648E-422.23
TACCTCT502.6950648E-422.26
GCGTCAG555.1298074E-420.1818184
TGCATCA1403.6379788E-1219.8214314
CACCGAG759.224961E-619.7333347
AAAAGTA500.00702197818.55
GCACCGA801.6095959E-518.56
TATACAC2200.018.537
CTGCATC1451.5279511E-1017.8620713
CTTATAC5600.017.83928737
GGTATCA12900.017.4961241
TAAAGCC852.7112745E-517.41176424
ATCAAGC1603.274181E-1117.3437530
CCAGGAC2300.016.8913043
GACAGGC1553.965397E-1016.7096797
ACGGGTT904.4267465E-516.4444458
AAAGCCC904.4267465E-516.44444525
GCTGCAT1606.220944E-1016.187512
ATAAAGC803.3711985E-416.187523
CAGGACA1951.8189894E-1216.1282064
Produced by FastQC (version 0.11.5)