##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631502.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	280928
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.01368322132361	34.0	33.0	34.0	31.0	34.0
2	33.14644321676729	34.0	33.0	34.0	31.0	34.0
3	33.209220867980406	34.0	33.0	34.0	31.0	34.0
4	36.44394293199681	37.0	37.0	37.0	35.0	37.0
5	36.44636348103429	37.0	37.0	37.0	35.0	37.0
6	36.512967735505185	37.0	37.0	37.0	35.0	37.0
7	36.53028534001595	37.0	37.0	37.0	35.0	37.0
8	36.5388604909443	37.0	37.0	37.0	35.0	37.0
9	38.37981974028933	39.0	39.0	39.0	37.0	39.0
10	38.35442533318145	39.0	39.0	39.0	37.0	39.0
11	38.40647781638	39.0	39.0	39.0	37.0	39.0
12	38.3818985647568	39.0	39.0	39.0	37.0	39.0
13	38.3978314728329	39.0	39.0	39.0	37.0	39.0
14	39.92591340129855	41.0	40.0	41.0	38.0	41.0
15	39.8950763184873	41.0	40.0	41.0	38.0	41.0
16	39.841607102175644	41.0	40.0	41.0	38.0	41.0
17	39.86576987697916	41.0	40.0	41.0	38.0	41.0
18	39.83657378403007	41.0	40.0	41.0	38.0	41.0
19	39.86218532862513	41.0	40.0	41.0	38.0	41.0
20	39.837182480920376	41.0	40.0	41.0	38.0	41.0
21	39.809734166761594	41.0	40.0	41.0	38.0	41.0
22	39.74498803964005	41.0	40.0	41.0	37.0	41.0
23	39.692024290921516	41.0	40.0	41.0	37.0	41.0
24	39.65069697573755	41.0	40.0	41.0	37.0	41.0
25	39.646040978471355	41.0	40.0	41.0	37.0	41.0
26	39.51316707483768	41.0	40.0	41.0	36.0	41.0
27	39.44361544595056	41.0	39.0	41.0	36.0	41.0
28	39.37618891673311	41.0	39.0	41.0	35.0	41.0
29	39.303576005239776	41.0	39.0	41.0	35.0	41.0
30	39.20196278049892	41.0	39.0	41.0	35.0	41.0
31	39.10718404715799	41.0	39.0	41.0	35.0	41.0
32	38.99906025743251	41.0	39.0	41.0	35.0	41.0
33	38.88681797471238	41.0	38.0	41.0	35.0	41.0
34	38.785119318829025	41.0	38.0	41.0	35.0	41.0
35	38.65478343205376	41.0	38.0	41.0	35.0	41.0
36	38.56888953753275	40.0	38.0	41.0	35.0	41.0
37	38.451432395489235	40.0	38.0	41.0	35.0	41.0
38	38.34479297186468	40.0	38.0	41.0	35.0	41.0
39	38.196192618749286	40.0	38.0	41.0	35.0	41.0
40	38.03478827315184	40.0	37.0	41.0	35.0	41.0
41	37.863238979382615	40.0	37.0	41.0	34.0	41.0
42	37.67813817063447	40.0	37.0	41.0	34.0	41.0
43	36.88124359266431	40.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	0.0
16	1.0
17	1.0
18	3.0
19	14.0
20	21.0
21	41.0
22	63.0
23	118.0
24	189.0
25	308.0
26	463.0
27	756.0
28	1048.0
29	1577.0
30	1978.0
31	2507.0
32	3116.0
33	3788.0
34	5647.0
35	8620.0
36	12240.0
37	26500.0
38	49498.0
39	162429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.843262330561565	17.480279644606448	13.477118692333978	25.199339332498006
2	19.378274860462465	20.24504499373505	34.21695238637658	26.159727759425905
3	21.099000455632762	22.2153007176216	28.97681968333523	27.70887914341041
4	15.143381934161065	16.366827087367582	32.340670919239095	36.14912005923226
5	15.598302767969017	35.99712381820253	31.652238295933476	16.752335117894976
6	33.39859323385351	32.27197004214603	16.931384554049437	17.39805216995102
7	29.321035994988037	30.343362000227813	20.400244902608495	19.935357102175647
8	29.160496639708395	31.37173937806128	19.365460189087596	20.102303793142724
9	27.75408645631621	14.127463264608725	19.796531495614538	38.32191878346053
10	20.0300432851122	26.167558947488324	29.73395318373391	24.068444583665567
11	34.4501082127805	22.733582982116417	19.519948171773553	23.296360633329535
12	22.832540722177924	26.939642897824356	26.81292003645062	23.414896343547102
13	31.157093632532177	19.208124501651668	25.184388882560654	24.450392983255494
14	23.54161920492083	20.99897482629001	25.342436496184074	30.11696947260508
15	27.342593119945324	25.932623305615678	22.38509511333865	24.339688461100355
16	24.580675475566693	25.165522838592096	24.89926244446976	25.354539241371455
17	23.052525914113225	27.01190340585488	25.368065838933816	24.567504841098074
18	22.875612256521244	23.784386034855906	27.563646201161863	25.776355507460984
19	23.953824467479212	25.68843262330562	27.73522041234765	22.622522496867525
20	24.657563503815926	24.153519763070967	27.90074325093974	23.28817348217337
21	26.41388540836086	23.773707142043513	26.584391730265406	23.22801571933022
22	25.97818658161522	24.118635379883813	26.04688745870828	23.856290579792688
23	24.702414853627978	24.68604055131564	26.036564528989636	24.57498006606675
24	23.630254015263695	25.428223601776967	27.15820423738467	23.78331814557467
25	23.852374985761475	24.78286251281467	26.799037475794506	24.56572502562934
26	24.63015434559745	24.758301059346167	26.833209932794166	23.778334662262214
27	24.736231347533888	24.42405171431826	26.329166192049207	24.510550746098644
28	23.224812051486502	24.52514523294225	27.79395432281581	24.45608839275544
29	23.460459619546643	24.7049065952842	28.364563162091354	23.4700706230778
30	22.882375555302424	25.21784941337282	28.004684474313702	23.89509055701105
31	23.135465314956143	25.463463948057864	27.198072103884268	24.202998633101718
32	22.596537191024034	25.148792573186014	28.20829536393667	24.046374871853285
33	23.163586399362117	25.041647681968332	27.799293769222004	23.995472149447544
34	23.800760337168242	24.69814329650302	27.44083893381934	24.060257432509395
35	23.240118464517597	24.68426073584691	27.62202414853628	24.453596651099215
36	22.289341041120856	25.007831188062426	27.25182253103998	25.45100523977674
37	22.30713919580818	23.944213463948056	28.480963093746443	25.267684246497325
38	22.477289554618977	23.019421346394804	29.29077913201959	25.21250996696662
39	21.847234878687775	23.275714773892243	30.201332725822986	24.675717621596995
40	21.69274689600182	22.783417815240917	30.382517940539927	25.141317348217335
41	20.23329821164142	23.336584462922886	31.25427155712496	25.17584576831074
42	20.221195466454038	23.72600808748149	30.58114534685044	25.471651099214032
43	19.491827087367582	23.290309260735846	31.203012871625468	26.014850780271104
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	134.5
2	135.0
3	275.5
4	416.0
5	416.0
6	538.5
7	661.0
8	658.0
9	655.0
10	1014.0
11	1373.0
12	1373.0
13	2331.5
14	3290.0
15	4331.0
16	5372.0
17	4787.5
18	4203.0
19	4203.0
20	4608.0
21	5013.0
22	3925.0
23	2837.0
24	2858.5
25	2880.0
26	2880.0
27	3228.5
28	3577.0
29	4292.5
30	5008.0
31	5528.0
32	6048.0
33	6048.0
34	6811.0
35	7574.0
36	8343.0
37	9112.0
38	10043.5
39	10975.0
40	10975.0
41	11937.0
42	12899.0
43	14267.5
44	15636.0
45	16717.5
46	17799.0
47	17799.0
48	19435.5
49	21072.0
50	21004.0
51	20936.0
52	20555.5
53	20175.0
54	20175.0
55	19360.5
56	18546.0
57	17337.0
58	16128.0
59	15111.0
60	14094.0
61	14094.0
62	13612.5
63	13131.0
64	11986.0
65	10841.0
66	9620.0
67	8399.0
68	8399.0
69	7281.5
70	6164.0
71	5635.0
72	5106.0
73	4707.5
74	4309.0
75	4309.0
76	3791.5
77	3274.0
78	2618.0
79	1962.0
80	1260.0
81	558.0
82	558.0
83	422.5
84	287.0
85	233.0
86	179.0
87	143.5
88	108.0
89	108.0
90	76.0
91	44.0
92	26.0
93	8.0
94	8.0
95	8.0
96	8.0
97	4.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	280928.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.87556499864759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.96167557756462	57.714389663208934
2	5.464892683299941	7.090760009721178
3	1.7737068946396217	3.4521071079522647
4	0.9366062963574286	2.4305145062991578
5	0.5834777964837253	1.8926725855523796
6	0.4246177592590016	1.652839022423247
7	0.29438151074045227	1.33687167841096
8	0.22885905430584083	1.1877888362518076
9	0.17177273749226118	1.0029468056557336
>10	1.0451256873242862	12.68764758644033
>50	0.0830023176194242	3.681649332923367
>100	0.02968294802284044	3.2073570418745407
>500	0.0	0.0
>1k	0.0021987368905807734	2.6624558232861153
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2475	0.8810086570224399	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2223	0.791305957398337	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1707	0.6076290010251737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.37767684246497324	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	424	0.1509283517484907	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	414	0.1473687208110263	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	366	0.13028249231119718	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	331	0.11782378403007177	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	296	0.10536507574894635	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.5596309374644035E-4	0.0
11	0.0	0.0	0.0	0.0010678892812393211	0.0
12	0.0	0.0	0.0	0.0017798154687322018	0.0
13	0.0	0.0	0.0	0.0021357785624786423	0.0
14	0.0	0.0	0.0	0.0028477047499715228	0.0
15	0.0	0.0	0.0	0.004271557124957285	0.0
16	0.0	0.0	0.0	0.00996696662490033	0.0
17	0.0	0.0	0.0	0.013526597562364734	0.0
18	0.0	0.0	0.0	0.015306413031096935	0.0
19	0.0	0.0	0.0	0.0185100808748149	0.0
20	0.0	0.0	0.0	0.02135778562478642	0.0
21	0.0	0.0	0.0	0.026697232030983026	0.0
22	0.0	0.0	0.0	0.03808805103086912	0.0
23	0.0	0.0	0.0	0.04947887003075521	0.0
24	0.0	0.0	0.0	0.06905684018680944	0.0
25	0.0	0.0	0.0	0.07831188062421689	0.0
26	0.0	0.0	0.0	0.0961100353115389	0.0
27	0.0	0.0	0.0	0.17050632190454493	0.0
28	0.0	0.0	0.0	0.36628602346508715	0.0
29	0.0	0.0	0.0	0.6460730151497893	0.0
30	0.0	0.0	0.0	1.0315810456771841	0.0
31	0.0	0.0	0.0	1.8567034969814329	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTCA	50	2.6950648E-4	22.2	3
TACCTCT	50	2.6950648E-4	22.2	6
GCGTCAG	55	5.1298074E-4	20.181818	4
TGCATCA	140	3.6379788E-12	19.82143	14
CACCGAG	75	9.224961E-6	19.733334	7
AAAAGTA	50	0.007021978	18.5	5
GCACCGA	80	1.6095959E-5	18.5	6
TATACAC	220	0.0	18.5	37
CTGCATC	145	1.5279511E-10	17.86207	13
CTTATAC	560	0.0	17.839287	37
GGTATCA	1290	0.0	17.496124	1
TAAAGCC	85	2.7112745E-5	17.411764	24
ATCAAGC	160	3.274181E-11	17.34375	30
CCAGGAC	230	0.0	16.891304	3
GACAGGC	155	3.965397E-10	16.709679	7
ACGGGTT	90	4.4267465E-5	16.444445	8
AAAGCCC	90	4.4267465E-5	16.444445	25
GCTGCAT	160	6.220944E-10	16.1875	12
ATAAAGC	80	3.3711985E-4	16.1875	23
CAGGACA	195	1.8189894E-12	16.128206	4
>>END_MODULE