FastQCFastQC Report
Fri 10 Feb 2017
ERR1631501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80288
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1100.13700677560781188No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC970.1208150657632523No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC830.10337783977680351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTC200.001831753637.04
TCCTACC200.001831753637.033
ATACACA551.810622E-830.27272837
ACCATAG250.00546630829.59999831
TATTAGC250.00546630829.5999982
TAAGAGG250.00546630829.5999984
GTATTAG358.7974075E-426.428571
GCCTCCT400.001915661223.12500230
AAGCCTC450.003795343720.55555528
CTACAAC450.003795343720.5555553
TACTGTC609.1351673E-418.535
GTATCAA609.1351673E-418.51
GTCTACA500.00697940118.4999981
AACTGTC500.00697940118.49999836
AGCCTCC500.00697940118.49999829
CTTATAC3250.018.21538537
TATACAC953.5226149E-617.52631636
TGCTGAG650.001562689517.0769237
TCCTGGA700.0025644315.8571422
TTATACA1659.222276E-1015.69696937
Produced by FastQC (version 0.11.5)