FastQCFastQC Report
Fri 10 Feb 2017
ERR1631500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606525
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.22257944849758873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12780.21070854457771732No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10770.17756893780140967No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA8420.13882362639627385No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC6760.11145459791434814No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT6080.1002431886566918No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC6070.10007831499113805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACC358.864547E-426.428574
AATTTCG400.001930141823.12528
GTCTAGA1850.023.01
GTCCTAA502.7000633E-422.21
GCAGTCG2350.022.0425559
CTAGAAC2200.021.8636363
CGCCTTA2150.021.51162725
GACATGT1301.8189894E-1221.3461537
CTAGACA2000.021.2750024
AGTCGGT2450.021.14285911
ACTGATC1600.020.81258
TTTCGGT450.00382375520.55555730
TCTATGT450.00382375520.5555573
TCTAGAC1900.020.4473693
GTATTAA555.139245E-420.1818181
ATTTCGG555.139245E-420.18181829
GGTATCA7350.020.1360531
CTCTAGT1750.020.08571427
CCTAAAC656.8952635E-519.9230773
TAGAACT2200.019.3409084
Produced by FastQC (version 0.11.5)