##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631500.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606525
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.09204072379539	34.0	33.0	34.0	31.0	34.0
2	33.23542310704423	34.0	33.0	34.0	31.0	34.0
3	33.3181682535757	34.0	34.0	34.0	31.0	34.0
4	36.5194954865834	37.0	37.0	37.0	35.0	37.0
5	36.514438811260874	37.0	37.0	37.0	35.0	37.0
6	36.56507151395243	37.0	37.0	37.0	35.0	37.0
7	36.578131157000946	37.0	37.0	37.0	35.0	37.0
8	36.57348007089568	37.0	37.0	37.0	35.0	37.0
9	38.42995259882115	39.0	39.0	39.0	37.0	39.0
10	38.401807015374466	39.0	39.0	39.0	37.0	39.0
11	38.452858497176535	39.0	39.0	39.0	37.0	39.0
12	38.4202168088702	39.0	39.0	39.0	37.0	39.0
13	38.43604138329005	39.0	39.0	39.0	37.0	39.0
14	40.00069082065867	41.0	40.0	41.0	38.0	41.0
15	39.98973001937266	41.0	40.0	41.0	38.0	41.0
16	39.96014014261572	41.0	40.0	41.0	38.0	41.0
17	39.957012489180165	41.0	40.0	41.0	38.0	41.0
18	39.93902806974156	41.0	40.0	41.0	38.0	41.0
19	39.946823296648944	41.0	40.0	41.0	38.0	41.0
20	39.91333580643832	41.0	40.0	41.0	38.0	41.0
21	39.88437739582045	41.0	40.0	41.0	38.0	41.0
22	39.83646675734718	41.0	40.0	41.0	38.0	41.0
23	39.786976629157905	41.0	40.0	41.0	38.0	41.0
24	39.75714768558592	41.0	40.0	41.0	38.0	41.0
25	39.74591154527843	41.0	40.0	41.0	37.0	41.0
26	39.661084044351014	41.0	40.0	41.0	37.0	41.0
27	39.609331849470344	41.0	40.0	41.0	37.0	41.0
28	39.55137380981823	41.0	40.0	41.0	37.0	41.0
29	39.50996743745105	41.0	40.0	41.0	37.0	41.0
30	39.44472527925477	41.0	39.0	41.0	36.0	41.0
31	39.39834301966118	41.0	39.0	41.0	36.0	41.0
32	39.32531717571411	41.0	39.0	41.0	36.0	41.0
33	39.25351469436544	41.0	39.0	41.0	35.0	41.0
34	39.19835785829108	41.0	39.0	41.0	35.0	41.0
35	39.11266312188286	41.0	39.0	41.0	35.0	41.0
36	39.05151477680228	41.0	39.0	41.0	35.0	41.0
37	39.003980050286465	41.0	39.0	41.0	35.0	41.0
38	38.93440666089609	41.0	39.0	41.0	35.0	41.0
39	38.846665842298336	40.0	39.0	41.0	35.0	41.0
40	38.752461976010885	40.0	38.0	41.0	35.0	41.0
41	38.65905774700136	40.0	38.0	41.0	35.0	41.0
42	38.526126705411976	40.0	38.0	41.0	35.0	41.0
43	37.74222002390668	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	1.0
17	3.0
18	6.0
19	8.0
20	29.0
21	72.0
22	97.0
23	182.0
24	313.0
25	466.0
26	701.0
27	1012.0
28	1467.0
29	2021.0
30	2752.0
31	3742.0
32	4940.0
33	6286.0
34	9618.0
35	14270.0
36	24111.0
37	46832.0
38	110738.0
39	376854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.064465603231525	17.12015168377231	11.541321462429414	27.274061250566756
2	19.896789085363338	19.6565681546515	32.52726598244096	27.91937677754421
3	20.79914265693912	20.378055315114793	28.127777090804173	30.695024937141913
4	15.241086517455999	14.50311199043733	31.92959894480854	38.32620254729813
5	17.020073368781173	34.27904867894975	31.45459791434813	17.246280037920943
6	36.903672560900205	32.115411565887634	15.051152054738056	15.929763818474093
7	31.957297720621575	28.484069082065865	19.249165327068134	20.309467870244426
8	29.314372861794652	30.384897572235275	19.160957915996867	21.13977164997321
9	27.80924116895429	13.326573513045629	18.570380445983265	40.29380487201682
10	18.89501669345864	24.865256996826183	30.28498413090969	25.95474217880549
11	38.43666790321916	20.43378261407197	19.22179629858621	21.907753184122665
12	24.123490375499774	24.113927702897655	26.503606611433987	25.258975310168587
13	31.98153414945798	17.601253039858207	23.779399035489057	26.637813775194758
14	24.49742384897572	19.062858084992374	22.665182803676682	33.77453526235522
15	27.6872346564445	25.59086599892832	20.434112361403077	26.287786983224105
16	27.429866864515066	23.73455339845843	22.159185524092166	26.67639421293434
17	25.850706895841064	24.59618317464243	22.89254358847533	26.660566341041175
18	26.652157784097934	22.207328634433864	24.561394831210585	26.579118750257614
19	26.32521330530481	23.544454062074934	23.73372903013066	26.396603602489595
20	26.516796504678293	22.932278141873788	24.215489880878778	26.335435472569145
21	28.033963975104076	22.947281645439183	24.00082436832777	25.017930011128975
22	27.72532047318742	22.545319648819092	22.915955648983967	26.813404229009517
23	26.386546308890814	22.86979102262891	23.70388689666543	27.039775771814845
24	26.09208194221178	23.331437286179465	23.516095791599685	27.06038498000907
25	26.718436997650553	22.944313919459212	23.60891966530646	26.728329417583772
26	27.018177321627302	23.242570380445983	23.249495074399242	26.489757223527473
27	26.900787271753018	22.466180289353282	23.68014508882569	26.95288735006801
28	25.83933061291785	22.81933968096946	24.42553893079428	26.91579077531841
29	25.619883764065786	23.047854581426982	24.11458719755987	27.217674456947368
30	24.822884464778863	23.920530893203082	25.37636536004287	25.880219281975187
31	26.770701949631093	23.354189852025886	23.309509088660814	26.565599109682207
32	25.809323605787064	23.15716582168913	23.805119327315445	27.22839124520836
33	25.657310086146488	22.513828778698322	24.552821400601786	27.276039734553397
34	26.74712501545691	22.246898314166767	24.091999505379004	26.91397716499732
35	25.90626932113268	22.723712954948272	25.050410123243065	26.319607600675983
36	25.09542063393924	22.68612175920201	25.454515477515354	26.763942129343395
37	26.03618976958905	22.206834013437206	25.237541733646594	26.51943448332715
38	24.990890729978155	21.43670912163555	26.401055191459545	27.171344956926756
39	25.484357610980584	21.363834961460782	26.51910473599604	26.63270269156259
40	24.717200445158895	22.263880301718807	26.54070318618359	26.47821606693871
41	24.376241704793703	21.69523102922386	27.670747289889125	26.25777997609332
42	24.73879889534644	21.800915048843823	27.337207864473847	26.123078191335892
43	23.064012200651252	21.51238613412473	27.58847533077779	27.83512633444623
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	53.5
2	57.0
3	142.0
4	227.0
5	227.0
6	276.5
7	326.0
8	297.0
9	268.0
10	433.0
11	598.0
12	598.0
13	1026.0
14	1454.0
15	2076.5
16	2699.0
17	2525.5
18	2352.0
19	2352.0
20	2653.5
21	2955.0
22	2398.5
23	1842.0
24	2051.5
25	2261.0
26	2261.0
27	2762.0
28	3263.0
29	4058.5
30	4854.0
31	5959.5
32	7065.0
33	7065.0
34	8966.0
35	10867.0
36	13750.0
37	16633.0
38	19145.0
39	21657.0
40	21657.0
41	23975.5
42	26294.0
43	29100.5
44	31907.0
45	36235.0
46	40563.0
47	40563.0
48	43561.0
49	46559.0
50	48441.0
51	50323.0
52	51343.0
53	52363.0
54	52363.0
55	50773.5
56	49184.0
57	47695.0
58	46206.0
59	45148.0
60	44090.0
61	44090.0
62	41444.0
63	38798.0
64	35165.5
65	31533.0
66	28331.5
67	25130.0
68	25130.0
69	20733.5
70	16337.0
71	13916.5
72	11496.0
73	8631.5
74	5767.0
75	5767.0
76	4532.5
77	3298.0
78	2812.5
79	2327.0
80	2073.5
81	1820.0
82	1820.0
83	1464.0
84	1108.0
85	1015.5
86	923.0
87	789.0
88	655.0
89	655.0
90	488.5
91	322.0
92	192.0
93	62.0
94	43.0
95	24.0
96	24.0
97	15.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	606525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28784144672972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.10992929418772	41.948847005943016
2	7.089909683237204	6.988926886780581
3	2.4136989464460945	3.5689803291772106
4	1.2456981711454043	2.4559109579958345
5	0.7592448756619812	1.8710770525434874
6	0.49106177558022135	1.4522024961208513
7	0.3621167843530987	1.24935682566766
8	0.2637331069368789	1.039906844716664
9	0.21037900289627595	0.9332214244625467
>10	1.6791219912525215	17.09005247723171
>50	0.23196926492652475	8.040790105980793
>100	0.13877929929207145	11.755940079288518
>500	0.003352156987731194	0.9926452573519798
>1k	0.0010056470963193582	0.6121422567391952
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1350	0.22257944849758873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1278	0.21070854457771732	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1077	0.17756893780140967	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	842	0.13882362639627385	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	676	0.11145459791434814	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	608	0.1002431886566918	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	607	0.10007831499113805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.6487366555376944E-4	0.0
14	0.0	0.0	0.0	4.946209966613083E-4	0.0
15	0.0	0.0	0.0	9.892419933226165E-4	0.0
16	0.0	0.0	0.0	0.00280285231441408	0.0
17	0.0	0.0	0.0	0.003956967973290466	0.0
18	0.0	0.0	0.0	0.0041218416388442355	0.0
19	0.0	0.0	0.0	0.00560570462882816	0.0
20	0.0	0.0	0.0	0.006100325625489469	0.0
21	0.0	0.0	0.0	0.007913935946580932	0.0
22	0.0	0.0	0.0	0.009562672602118627	0.0
23	0.0	0.0	0.0	0.012530398582086476	0.0
24	0.0	0.0	0.0	0.017806355879807098	0.0
25	0.0	0.0	0.0	0.020938955525328717	0.0
26	0.0	0.0	0.0	0.029842133465232266	0.0
27	0.0	0.0	0.0	0.059024772268249454	0.0
28	0.0	0.0	0.0	0.15877333992827997	0.0
29	0.0	0.0	0.0	0.3008944396356292	0.0
30	0.0	0.0	0.0	0.496269733316846	0.0
31	0.0	0.0	0.0	0.9673137958039653	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACACC	35	8.864547E-4	26.42857	4
AATTTCG	40	0.0019301418	23.125	28
GTCTAGA	185	0.0	23.0	1
GTCCTAA	50	2.7000633E-4	22.2	1
GCAGTCG	235	0.0	22.042555	9
CTAGAAC	220	0.0	21.863636	3
CGCCTTA	215	0.0	21.511627	25
GACATGT	130	1.8189894E-12	21.346153	7
CTAGACA	200	0.0	21.275002	4
AGTCGGT	245	0.0	21.142859	11
ACTGATC	160	0.0	20.8125	8
TTTCGGT	45	0.003823755	20.555557	30
TCTATGT	45	0.003823755	20.555557	3
TCTAGAC	190	0.0	20.447369	3
GTATTAA	55	5.139245E-4	20.181818	1
ATTTCGG	55	5.139245E-4	20.181818	29
GGTATCA	735	0.0	20.136053	1
CTCTAGT	175	0.0	20.085714	27
CCTAAAC	65	6.8952635E-5	19.923077	3
TAGAACT	220	0.0	19.340908	4
>>END_MODULE