FastQCFastQC Report
Fri 10 Feb 2017
ERR1631499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328543
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7130.2170187768419963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6050.18414636744657473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4570.13909899160840436No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4220.12844589597099923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAG250.00549070129.62
GGGTGAA250.00549070129.611
GACTGTA250.00549070129.67
GGTATCA4550.023.1758251
GCCTTAT400.001928268223.12526
CATAGAA400.001928268223.1252
TTATGCG450.003820080820.5555554
GATCAAC450.003820080820.55555518
ACGCTAT555.132354E-420.18181827
AGTCGGT555.132354E-420.18181811
TTATACA2950.019.44067837
TACGCTA609.2176854E-418.526
TGGTCAG1409.276846E-1118.510
TATACAC1451.5279511E-1017.86206837
GCAGTCG650.001576688717.0769239
TTGAGAG650.001576688717.0769235
ATACGCT650.001576688717.07692325
TACACCA650.001576688717.0769235
GTCGGTG650.001576688717.07692312
CTTATAC5300.016.75471537
Produced by FastQC (version 0.11.5)