##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631499.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328543
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05031609256627	34.0	33.0	34.0	31.0	34.0
2	33.19248621945986	34.0	33.0	34.0	31.0	34.0
3	33.27498379207592	34.0	34.0	34.0	31.0	34.0
4	36.49559114027692	37.0	37.0	37.0	35.0	37.0
5	36.48616162876701	37.0	37.0	37.0	35.0	37.0
6	36.534450589420565	37.0	37.0	37.0	35.0	37.0
7	36.5438892321553	37.0	37.0	37.0	35.0	37.0
8	36.54532587819555	37.0	37.0	37.0	35.0	37.0
9	38.39402148272829	39.0	39.0	39.0	37.0	39.0
10	38.36171825301407	39.0	39.0	39.0	37.0	39.0
11	38.413142267526624	39.0	39.0	39.0	37.0	39.0
12	38.37981938437282	39.0	39.0	39.0	37.0	39.0
13	38.4012290628624	39.0	39.0	39.0	37.0	39.0
14	39.95179930785316	41.0	40.0	41.0	38.0	41.0
15	39.94247328355801	41.0	40.0	41.0	38.0	41.0
16	39.90469740642777	41.0	40.0	41.0	38.0	41.0
17	39.90432606995127	41.0	40.0	41.0	38.0	41.0
18	39.86989830859279	41.0	40.0	41.0	38.0	41.0
19	39.88212806238453	41.0	40.0	41.0	38.0	41.0
20	39.84654367921399	41.0	40.0	41.0	38.0	41.0
21	39.81001573614413	41.0	40.0	41.0	38.0	41.0
22	39.75391044703433	41.0	40.0	41.0	38.0	41.0
23	39.707441643863966	41.0	40.0	41.0	37.0	41.0
24	39.668889612623005	41.0	40.0	41.0	37.0	41.0
25	39.663523496163364	41.0	40.0	41.0	37.0	41.0
26	39.5732796011481	41.0	40.0	41.0	37.0	41.0
27	39.5261168248905	41.0	40.0	41.0	37.0	41.0
28	39.45600119314671	41.0	39.0	41.0	36.0	41.0
29	39.40924019078172	41.0	39.0	41.0	36.0	41.0
30	39.351737824272625	41.0	39.0	41.0	36.0	41.0
31	39.31147521024645	41.0	39.0	41.0	35.0	41.0
32	39.238370624240964	41.0	39.0	41.0	35.0	41.0
33	39.160466057715425	41.0	39.0	41.0	35.0	41.0
34	39.10835111385724	41.0	39.0	41.0	35.0	41.0
35	39.0104796023656	41.0	39.0	41.0	35.0	41.0
36	38.95472129979942	41.0	39.0	41.0	35.0	41.0
37	38.882767248122775	41.0	39.0	41.0	35.0	41.0
38	38.808731277184414	40.0	38.0	41.0	35.0	41.0
39	38.732172044450806	40.0	38.0	41.0	35.0	41.0
40	38.62240254700298	40.0	38.0	41.0	35.0	41.0
41	38.53968582499095	40.0	38.0	41.0	35.0	41.0
42	38.409462383919305	40.0	37.0	41.0	35.0	41.0
43	37.61201121314409	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	1.0
16	0.0
17	2.0
18	3.0
19	6.0
20	23.0
21	44.0
22	60.0
23	117.0
24	199.0
25	302.0
26	441.0
27	601.0
28	861.0
29	1119.0
30	1629.0
31	2077.0
32	2828.0
33	3742.0
34	5895.0
35	8623.0
36	14130.0
37	26730.0
38	61967.0
39	197141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44149776437179	17.540474154068114	11.96647014241667	27.05155793914343
2	20.35319577650414	19.168571541624686	32.07464471926049	28.403587962610676
3	21.024036427499595	20.598521350325527	27.975029143825925	30.402413078348957
4	15.407115659137466	14.823630392368731	32.40062944576509	37.368624502728714
5	17.36576338561467	33.659825350106374	31.608647878664286	17.36576338561467
6	36.31092429301492	32.36227830147043	15.16148571115501	16.165311694359644
7	31.371540407191752	28.332973157242737	19.694225717790363	20.601260717775148
8	29.605257150509978	30.009770410570308	18.86602362552238	21.518948813397333
9	27.691961174032016	13.848719954465624	18.754318308410163	39.7050005630922
10	19.791321075171286	24.20565953315091	29.669175724334412	26.333843667343395
11	38.414454120160826	20.30571340737742	19.46533634866669	21.814496123795056
12	24.295754284827254	24.17278712375549	25.778360823392983	25.753097768024276
13	32.00555178469789	17.44155255172078	23.2054860398791	27.347409623702223
14	24.527078647239478	19.416332108734625	22.497511741233264	33.55907750279263
15	27.625911981080105	25.66087239722046	20.053082853690384	26.66013276800906
16	27.362628331755662	23.986510137181437	21.902460256343918	26.748401274718987
17	26.361541716000648	24.10856417576999	22.758664771430222	26.77122933679914
18	26.25166264385484	22.24366369090195	24.621130263009714	26.883543402233496
19	26.929503900554874	23.44868099457301	23.844063029801273	25.777752075070843
20	27.451201212626657	22.411069479489747	23.173222378805818	26.964506929077775
21	28.707353375357258	22.73614108351114	22.939158648943973	25.617346892187626
22	28.328407544826707	22.609217058345482	22.63417573955312	26.428199657274693
23	26.869542190824337	22.45094249458975	23.171091759678337	27.50842355490758
24	26.25683700459301	23.281275205985214	23.76462137376234	26.697266415659442
25	26.515250667340347	22.661265039888235	23.28279707679056	27.540687215980864
26	26.691483306599135	23.543645732826448	23.46785656672034	26.297014393854077
27	27.30053600289764	22.314278496269896	23.19909418249666	27.186091318335805
28	25.943940367014363	22.982988528137867	24.00416383852342	27.06890726632435
29	25.784448306614355	23.229531598603533	24.515816803279936	26.470203291502177
30	25.582648237825794	23.573170026450114	24.132914108655488	26.7112676270686
31	26.293057529760183	23.618826150610424	23.25844714390506	26.829669175724334
32	25.614911898899077	22.901720627132523	23.642262961012715	27.841104512955688
33	25.208268019711273	22.720313627135567	24.46924755663642	27.602170796516738
34	26.64095719586173	22.03699363553629	24.35967285865168	26.962376309950297
35	26.117738012984603	22.207443165734773	24.940114383809732	26.734704437470896
36	24.822017209315067	23.052385836861536	24.8071028754227	27.318494078400697
37	25.2819265666899	21.506469472793516	25.81184198111054	27.399761979406044
38	24.82840906669751	21.266927008032436	26.32562556499454	27.579038360275522
39	24.910894464347134	20.601565091936216	27.030251747868622	27.457288695848032
40	24.229096343553202	20.90654800132707	27.66730686698545	27.197048788134275
41	22.897459388877557	21.2468383134019	28.682090319988557	27.173611977731987
42	23.590823727792102	21.258100157361444	28.676307210928247	26.47476890391821
43	22.4981204895554	20.58573763556064	29.033033727700786	27.883108147183172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	31.0
2	28.0
3	61.5
4	95.0
5	95.0
6	123.0
7	151.0
8	146.5
9	142.0
10	219.0
11	296.0
12	296.0
13	486.5
14	677.0
15	1060.0
16	1443.0
17	1333.0
18	1223.0
19	1223.0
20	1420.0
21	1617.0
22	1504.5
23	1392.0
24	1610.0
25	1828.0
26	1828.0
27	2195.5
28	2563.0
29	3055.5
30	3548.0
31	4281.5
32	5015.0
33	5015.0
34	6166.5
35	7318.0
36	8647.0
37	9976.0
38	11278.0
39	12580.0
40	12580.0
41	13800.5
42	15021.0
43	16019.5
44	17018.0
45	18160.0
46	19302.0
47	19302.0
48	20855.0
49	22408.0
50	22909.0
51	23410.0
52	24267.5
53	25125.0
54	25125.0
55	24716.5
56	24308.0
57	24041.0
58	23774.0
59	23709.0
60	23644.0
61	23644.0
62	22319.0
63	20994.0
64	19451.0
65	17908.0
66	15951.0
67	13994.0
68	13994.0
69	12430.0
70	10866.0
71	9373.5
72	7881.0
73	6158.5
74	4436.0
75	4436.0
76	3698.0
77	2960.0
78	2492.0
79	2024.0
80	1713.0
81	1402.0
82	1402.0
83	1129.0
84	856.0
85	736.0
86	616.0
87	523.5
88	431.0
89	431.0
90	308.0
91	185.0
92	111.0
93	37.0
94	25.0
95	13.0
96	13.0
97	8.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	328543.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.24852707225823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2188480341037	57.96428100347064
2	4.919191592686173	6.321016284326454
3	1.5244703131496777	2.9383491655575313
4	0.73461888182419	1.887927244666942
5	0.4871118189040165	1.5648108442035829
6	0.33510219807367275	1.2917893586945766
7	0.24047743688307036	1.0815224779694452
8	0.19781985910207125	1.0167707658359788
9	0.14199301769684355	0.8210558017410565
>10	1.0049356159795102	12.696534105960728
>50	0.13551800731182106	5.948141265944468
>100	0.05896222072514231	6.065148872768061
>500	9.510035600829405E-4	0.4026528088605402
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	713	0.2170187768419963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	605	0.18414636744657473	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.13909899160840436	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	422	0.12844589597099923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.043741610687186E-4	0.0
10	0.0	0.0	0.0	3.043741610687186E-4	0.0
11	0.0	0.0	0.0	3.043741610687186E-4	0.0
12	0.0	0.0	0.0	3.043741610687186E-4	0.0
13	0.0	0.0	0.0	3.043741610687186E-4	0.0
14	0.0	0.0	0.0	3.043741610687186E-4	0.0
15	0.0	0.0	0.0	3.043741610687186E-4	0.0
16	0.0	0.0	0.0	6.087483221374372E-4	0.0
17	0.0	0.0	0.0	6.087483221374372E-4	0.0
18	0.0	0.0	0.0	6.087483221374372E-4	0.0
19	0.0	0.0	0.0	9.131224832061557E-4	0.0
20	0.0	0.0	0.0	9.131224832061557E-4	0.0
21	0.0	0.0	0.0	9.131224832061557E-4	0.0
22	0.0	0.0	0.0	0.00213061912748103	0.0
23	0.0	0.0	0.0	0.003956864093893341	0.0
24	0.0	0.0	0.0	0.009131224832061557	0.0
25	0.0	0.0	0.0	0.013392463087023616	0.0
26	0.0	0.0	0.0	0.024045558724428765	0.0
27	0.0	0.0	0.0	0.07091917952901142	0.0
28	3.043741610687186E-4	0.0	0.0	0.22067126677482096	0.0
29	3.043741610687186E-4	3.043741610687186E-4	0.0	0.42368883220765624	0.0
30	3.043741610687186E-4	3.043741610687186E-4	0.0	0.6827112432771357	0.0
31	3.043741610687186E-4	3.043741610687186E-4	0.0	1.3447250436015985	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTAAG	25	0.005490701	29.6	2
GGGTGAA	25	0.005490701	29.6	11
GACTGTA	25	0.005490701	29.6	7
GGTATCA	455	0.0	23.175825	1
GCCTTAT	40	0.0019282682	23.125	26
CATAGAA	40	0.0019282682	23.125	2
TTATGCG	45	0.0038200808	20.555555	4
GATCAAC	45	0.0038200808	20.555555	18
ACGCTAT	55	5.132354E-4	20.181818	27
AGTCGGT	55	5.132354E-4	20.181818	11
TTATACA	295	0.0	19.440678	37
TACGCTA	60	9.2176854E-4	18.5	26
TGGTCAG	140	9.276846E-11	18.5	10
TATACAC	145	1.5279511E-10	17.862068	37
GCAGTCG	65	0.0015766887	17.076923	9
TTGAGAG	65	0.0015766887	17.076923	5
ATACGCT	65	0.0015766887	17.076923	25
TACACCA	65	0.0015766887	17.076923	5
GTCGGTG	65	0.0015766887	17.076923	12
CTTATAC	530	0.0	16.754715	37
>>END_MODULE