FastQCFastQC Report
Fri 10 Feb 2017
ERR1631498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26459
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7882.978192675460146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6752.5511168222532974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5041.904833893949129No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.8239162477795835No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2060.7785630598284138No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1320.4988850674628671No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1020.38550209758494275No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA880.33259004497524475No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC870.32881061264598055No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA860.32503118031671646No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.3099134509996599No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC790.2985751540118674No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT770.2910162893533391No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA720.2721191277070184No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.260780830719226No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT690.260780830719226No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA680.25700139838996183No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT680.25700139838996183No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT670.2532219660606977No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT670.2532219660606977No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.24188366907290526No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.24188366907290526No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG620.23432480441437695No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT580.21920707509732038No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA580.21920707509732038No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2116482104387921No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA510.19275104879247137No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC500.18897161646320723No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG490.18519218413394306No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT490.18519218413394306No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA440.16629502248762237No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT440.16629502248762237No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC410.15495672549982994No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT380.1436184285120375No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.13228013152424506No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.13228013152424506No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT340.12850069919498092No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT340.12850069919498092No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.12472126686571677No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.12094183453645263No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT320.12094183453645263No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA310.11716240220718847No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC300.11338296987792433No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA300.11338296987792433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG290.10960353754866019No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT290.10960353754866019No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA290.10960353754866019No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA290.10960353754866019No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT280.10582410521939604No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT270.1020446728901319No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT270.1020446728901319No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC270.1020446728901319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC251.201324E-436.99999637
GGGCAGT1400.022.4642857
TGGGCAG1400.022.4642856
GGCAGTC1400.022.4642858
AGTCGGT1500.020.96666711
GCAGTCG1550.020.2903239
CAGTCGG1600.019.6562510
ATTCCTC1550.019.09677519
GGTGATT1601.8189894E-1218.515
TGATTCC1601.8189894E-1218.517
GTGATTC1601.8189894E-1218.516
GATTCCT1601.8189894E-1218.518
GTCGGTG1700.018.512
TCGGTGA1651.8189894E-1217.93939613
TTCCTCG1257.528797E-917.76000220
GCCTTAT1155.7352736E-817.69565226
CGCCTTA1155.7352736E-817.69565225
TCGCCTT1155.7352736E-817.69565224
CTCGCCT1155.7352736E-817.69565223
TCCTCGC1301.2232704E-817.07692321
Produced by FastQC (version 0.11.5)