##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631498.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26459
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.9383574587097	34.0	33.0	34.0	31.0	34.0
2	33.032994444234475	34.0	33.0	34.0	31.0	34.0
3	33.01307683585925	34.0	33.0	34.0	31.0	34.0
4	36.336634037567556	37.0	37.0	37.0	35.0	37.0
5	36.370195396651425	37.0	37.0	37.0	35.0	37.0
6	36.45493026947352	37.0	37.0	37.0	35.0	37.0
7	36.46679768698741	37.0	37.0	37.0	35.0	37.0
8	36.487811330738126	37.0	37.0	37.0	35.0	37.0
9	38.31592274840319	39.0	39.0	39.0	37.0	39.0
10	38.279111077516156	39.0	39.0	39.0	37.0	39.0
11	38.334366378169996	39.0	39.0	39.0	37.0	39.0
12	38.33746551268	39.0	39.0	39.0	37.0	39.0
13	38.33867493102536	39.0	39.0	39.0	37.0	39.0
14	39.715484334253	41.0	40.0	41.0	37.0	41.0
15	39.67368381269134	41.0	39.0	41.0	37.0	41.0
16	39.55693714804036	41.0	39.0	41.0	37.0	41.0
17	39.55342227597415	41.0	39.0	41.0	37.0	41.0
18	39.713708001058244	41.0	40.0	41.0	38.0	41.0
19	39.769152273328544	41.0	40.0	41.0	38.0	41.0
20	39.785781775577306	41.0	40.0	41.0	38.0	41.0
21	39.78321176159341	41.0	40.0	41.0	37.0	41.0
22	39.686155939377905	41.0	40.0	41.0	37.0	41.0
23	39.632110057069426	41.0	40.0	41.0	37.0	41.0
24	39.55017196417098	41.0	40.0	41.0	36.0	41.0
25	39.514305151366266	41.0	40.0	41.0	36.0	41.0
26	39.315166861937335	41.0	39.0	41.0	35.0	41.0
27	39.17400506443932	41.0	39.0	41.0	35.0	41.0
28	38.998223666805245	41.0	38.0	41.0	35.0	41.0
29	38.8176801844363	40.0	38.0	41.0	35.0	41.0
30	38.568426622321326	40.0	37.0	41.0	35.0	41.0
31	38.27340413469897	40.0	37.0	41.0	35.0	41.0
32	38.05208057749726	40.0	35.0	41.0	35.0	41.0
33	37.82353830454666	40.0	35.0	41.0	35.0	41.0
34	37.66347934540232	40.0	35.0	41.0	35.0	41.0
35	37.442382554140366	40.0	35.0	41.0	34.0	41.0
36	37.234891719263764	40.0	35.0	41.0	33.0	41.0
37	36.99731660304622	40.0	35.0	41.0	33.0	41.0
38	36.73710268717639	39.0	35.0	41.0	33.0	41.0
39	36.421671264976	39.0	35.0	41.0	32.0	41.0
40	36.1032162969122	39.0	35.0	41.0	31.0	41.0
41	35.681167088703276	39.0	35.0	41.0	29.0	41.0
42	35.26901999319702	39.0	35.0	41.0	24.0	41.0
43	34.29139423258626	37.0	35.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	1.0
21	2.0
22	12.0
23	13.0
24	27.0
25	39.0
26	73.0
27	100.0
28	160.0
29	235.0
30	334.0
31	397.0
32	451.0
33	514.0
34	749.0
35	1063.0
36	1640.0
37	4565.0
38	5019.0
39	11062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20201065799917	14.520579009032843	16.497222117237992	23.780188215729996
2	19.097471559771723	21.773309648890738	33.198533580256246	25.930685211081293
3	19.902490645904987	23.523186817340036	30.01625155901584	26.558070977739145
4	13.107071317888053	19.150383612381418	30.87796213008806	36.86458293964247
5	13.398087607241393	40.8670017763332	29.075172909029064	16.65973770739635
6	27.873313428323065	30.961109641331873	21.508749385842247	19.656827544502818
7	30.571828111417666	32.58248611058619	17.07925469594467	19.766431082051476
8	36.0179900978873	28.742582864053816	16.342265391738163	18.897161646320722
9	32.17430741902566	12.354964284364488	17.25688801542008	38.21384028118977
10	21.58811746475679	28.19834460863978	22.362901092255942	27.85063683434748
11	33.18719528326846	27.03805888355569	15.321818662836842	24.452927170339013
12	25.1710193128992	31.308817415624173	24.184587474961262	19.335575796515364
13	35.93862201897275	18.281114176650668	25.424241278959904	20.356022525416684
14	23.961600967534675	21.833780566158964	26.50893835745871	27.695680108847654
15	32.416191088098564	24.679693110094863	21.047658641672022	21.85645716013455
16	22.84288899807249	25.994935560678783	27.090970936165387	24.071204505083337
17	17.990097887297328	31.25968479534374	27.880872292981596	22.86934502437734
18	22.476284062133868	22.393136550890056	31.229449336709624	23.90113005026645
19	21.304660040061982	29.524925356211497	32.16674855436714	17.003666049359385
20	24.188366907290526	22.74084432518236	34.18118598586493	18.889602781662195
21	27.060735477531274	21.198835934842585	31.966438640916135	19.773989946710007
22	25.794625647227786	21.86023659246381	28.134094259042293	24.21104350126611
23	21.648588382025018	25.446917872935487	28.682111946785593	24.222381798253902
24	20.613023923806644	24.89512075286292	33.667183189085	20.824672134245436
25	19.936505536868363	24.672134245436336	30.492460032503114	24.898900185192186
26	24.00317472315658	23.851997429986017	30.038928152991424	22.105899693865982
27	22.018972750292907	23.576098869949732	29.02226085641937	25.382667523337993
28	19.702180732453986	24.72126686571677	32.19698401300125	23.379568388827998
29	19.225972258966703	25.98737669602026	34.04134698968215	20.74530405533089
30	18.814014135076913	26.61854189500737	35.825239049094826	18.742204920820893
31	19.619033221210174	28.893760157224385	30.545372085112817	20.941834536452625
32	18.50788011640652	27.54072338334782	35.10714690653464	18.844249593711023
33	20.231301258550964	25.976038399032465	34.13583279791375	19.656827544502818
34	21.652367814354285	26.784836917494992	29.097849503004646	22.464945765146073
35	19.482973657356663	28.18700631165199	30.447106844551946	21.882913186439396
36	16.992327752371594	27.51426735704297	32.46910314070826	23.024301749877168
37	18.103480857175253	28.217241770286105	32.49177973468385	21.187497637854793
38	19.101250992100987	24.059866208095542	34.23031860614535	22.60856419365811
39	19.619033221210174	24.21482293359537	34.555349786462074	21.610794058732377
40	18.953853131259685	23.97293926452247	31.384406062209457	25.688801542008388
41	15.5372463056049	25.34865263237462	35.48509013946105	23.62901092255943
42	15.839600891946029	24.536074681582825	33.534903057560754	26.089421368910386
43	14.47144638875241	27.381987225518728	35.8176801844363	22.328886201292566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	18.5
4	35.0
5	35.0
6	63.0
7	91.0
8	99.0
9	107.0
10	189.5
11	272.0
12	272.0
13	456.5
14	641.0
15	1060.5
16	1480.0
17	1258.0
18	1036.0
19	1036.0
20	1196.0
21	1356.0
22	919.5
23	483.0
24	401.0
25	319.0
26	319.0
27	296.0
28	273.0
29	301.0
30	329.0
31	340.0
32	351.0
33	351.0
34	329.0
35	307.0
36	324.0
37	341.0
38	381.5
39	422.0
40	422.0
41	524.5
42	627.0
43	830.0
44	1033.0
45	1291.5
46	1550.0
47	1550.0
48	2285.5
49	3021.0
50	2882.0
51	2743.0
52	2205.5
53	1668.0
54	1668.0
55	1366.5
56	1065.0
57	1051.0
58	1037.0
59	1011.5
60	986.0
61	986.0
62	1004.0
63	1022.0
64	981.0
65	940.0
66	903.5
67	867.0
68	867.0
69	800.0
70	733.0
71	631.0
72	529.0
73	440.0
74	351.0
75	351.0
76	289.0
77	227.0
78	170.5
79	114.0
80	83.5
81	53.0
82	53.0
83	43.0
84	33.0
85	21.0
86	9.0
87	7.5
88	6.0
89	6.0
90	3.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26459.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.62984239767187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.24100201554852	59.88132582486111
2	4.428448027641808	5.8127669224082545
3	1.5202994529225455	2.9933104047772026
4	0.7313561762165275	1.9199516232661855
5	0.4434206737690757	1.4550814467666955
6	0.2591419522027066	1.020446728901319
7	0.24762453210480853	1.1376091311085075
8	0.10941549093003167	0.57447371404815
9	0.10365678088108264	0.6122680373407914
>10	0.7601497264612728	9.41078649986772
>50	0.1151742009789807	5.260969802335689
>100	0.02303484019579614	2.486866472655807
>500	0.017276130146847108	7.434143391662571
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	788	2.978192675460146	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	675	2.5511168222532974	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	504	1.904833893949129	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218	0.8239162477795835	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	206	0.7785630598284138	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	132	0.4988850674628671	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	102	0.38550209758494275	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	88	0.33259004497524475	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	87	0.32881061264598055	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	86	0.32503118031671646	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.3099134509996599	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	79	0.2985751540118674	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	77	0.2910162893533391	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	72	0.2721191277070184	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	0.260780830719226	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	69	0.260780830719226	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	68	0.25700139838996183	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	68	0.25700139838996183	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	67	0.2532219660606977	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	67	0.2532219660606977	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.24188366907290526	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64	0.24188366907290526	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	62	0.23432480441437695	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	58	0.21920707509732038	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	58	0.21920707509732038	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	56	0.2116482104387921	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	51	0.19275104879247137	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	50	0.18897161646320723	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	49	0.18519218413394306	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	49	0.18519218413394306	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	44	0.16629502248762237	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	44	0.16629502248762237	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	41	0.15495672549982994	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	38	0.1436184285120375	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.13228013152424506	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.13228013152424506	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	34	0.12850069919498092	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	34	0.12850069919498092	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33	0.12472126686571677	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32	0.12094183453645263	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	32	0.12094183453645263	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	31	0.11716240220718847	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	30	0.11338296987792433	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	30	0.11338296987792433	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	29	0.10960353754866019	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	29	0.10960353754866019	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	29	0.10960353754866019	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	29	0.10960353754866019	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	28	0.10582410521939604	No Hit
TCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCAT	27	0.1020446728901319	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	27	0.1020446728901319	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	27	0.1020446728901319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0037794323292641447	0.0
13	0.0	0.0	0.0	0.0037794323292641447	0.0
14	0.0	0.0	0.0	0.0037794323292641447	0.0
15	0.0	0.0	0.0	0.011338296987792434	0.0
16	0.0	0.0	0.0	0.015117729317056579	0.0
17	0.0	0.0	0.0	0.015117729317056579	0.0
18	0.0	0.0	0.0	0.015117729317056579	0.0
19	0.0	0.0	0.0	0.022676593975584868	0.0
20	0.0	0.0	0.0	0.030235458634113158	0.0
21	0.0	0.0	0.0	0.037794323292641444	0.0
22	0.0	0.0	0.0	0.056691484938962165	0.0
23	0.0	0.0	0.0	0.06425034959749046	0.0
24	0.0	0.0	0.0	0.09448580823160362	0.0
25	0.0	0.0	0.0	0.10960353754866019	0.0
26	0.0	0.0	0.0	0.12850069919498092	0.0
27	0.0	0.0	0.0	0.18897161646320723	0.0
28	0.0	0.0	0.0	0.3703843682678862	0.0
29	0.0	0.0	0.0	0.7294304395479799	0.0
30	0.0	0.0	0.0	1.1035942401451302	0.0
31	0.0	0.0	0.0	2.03333459314411	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCCCC	25	1.201324E-4	36.999996	37
GGGCAGT	140	0.0	22.464285	7
TGGGCAG	140	0.0	22.464285	6
GGCAGTC	140	0.0	22.464285	8
AGTCGGT	150	0.0	20.966667	11
GCAGTCG	155	0.0	20.290323	9
CAGTCGG	160	0.0	19.65625	10
ATTCCTC	155	0.0	19.096775	19
GGTGATT	160	1.8189894E-12	18.5	15
TGATTCC	160	1.8189894E-12	18.5	17
GTGATTC	160	1.8189894E-12	18.5	16
GATTCCT	160	1.8189894E-12	18.5	18
GTCGGTG	170	0.0	18.5	12
TCGGTGA	165	1.8189894E-12	17.939396	13
TTCCTCG	125	7.528797E-9	17.760002	20
GCCTTAT	115	5.7352736E-8	17.695652	26
CGCCTTA	115	5.7352736E-8	17.695652	25
TCGCCTT	115	5.7352736E-8	17.695652	24
CTCGCCT	115	5.7352736E-8	17.695652	23
TCCTCGC	130	1.2232704E-8	17.076923	21
>>END_MODULE