##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631497.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7565
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79748843357568	34.0	31.0	34.0	31.0	34.0
2	32.949636483807005	34.0	33.0	34.0	31.0	34.0
3	33.00356906807667	34.0	33.0	34.0	31.0	34.0
4	36.284996695307335	37.0	37.0	37.0	35.0	37.0
5	36.296232650363514	37.0	37.0	37.0	35.0	37.0
6	36.36259087904825	37.0	37.0	37.0	35.0	37.0
7	36.391011235955055	37.0	37.0	37.0	35.0	37.0
8	36.39762062128222	37.0	37.0	37.0	35.0	37.0
9	38.199867812293455	39.0	39.0	39.0	37.0	39.0
10	38.14897554527429	39.0	39.0	39.0	37.0	39.0
11	38.233575677462	39.0	39.0	39.0	37.0	39.0
12	38.17541308658295	39.0	39.0	39.0	37.0	39.0
13	38.210575016523464	39.0	39.0	39.0	37.0	39.0
14	39.68010575016523	41.0	40.0	41.0	37.0	41.0
15	39.620753469927294	41.0	40.0	41.0	37.0	41.0
16	39.58149372108394	41.0	39.0	41.0	37.0	41.0
17	39.55280898876404	41.0	39.0	41.0	37.0	41.0
18	39.592465300727035	41.0	40.0	41.0	37.0	41.0
19	39.61493721083939	41.0	40.0	41.0	37.0	41.0
20	39.620224719101124	41.0	40.0	41.0	37.0	41.0
21	39.59087904824851	41.0	40.0	41.0	37.0	41.0
22	39.50416391275611	41.0	39.0	41.0	37.0	41.0
23	39.439656311962985	41.0	39.0	41.0	36.0	41.0
24	39.37554527428949	41.0	39.0	41.0	36.0	41.0
25	39.28393919365499	41.0	39.0	41.0	36.0	41.0
26	39.22908129543952	41.0	39.0	41.0	35.0	41.0
27	39.05657633840053	41.0	39.0	41.0	35.0	41.0
28	38.955717118307994	41.0	39.0	41.0	35.0	41.0
29	38.874818241903505	40.0	38.0	41.0	35.0	41.0
30	38.7035029742234	40.0	38.0	41.0	35.0	41.0
31	38.58506278916061	40.0	38.0	41.0	35.0	41.0
32	38.52240581625909	40.0	38.0	41.0	35.0	41.0
33	38.34976867151355	40.0	37.0	41.0	35.0	41.0
34	38.184534038334434	40.0	37.0	41.0	34.0	41.0
35	38.06847323198942	40.0	37.0	41.0	34.0	41.0
36	37.92412425644415	40.0	36.0	41.0	34.0	41.0
37	37.78294778585592	40.0	36.0	41.0	33.0	41.0
38	37.62247191011236	40.0	36.0	41.0	33.0	41.0
39	37.45076007931262	40.0	35.0	41.0	33.0	41.0
40	37.263185723727695	40.0	35.0	41.0	33.0	41.0
41	37.06186384666226	39.0	35.0	41.0	33.0	41.0
42	36.84018506278916	39.0	35.0	41.0	32.0	41.0
43	35.96219431592862	38.0	35.0	41.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	3.0
24	5.0
25	12.0
26	15.0
27	34.0
28	32.0
29	51.0
30	75.0
31	91.0
32	135.0
33	149.0
34	197.0
35	267.0
36	459.0
37	936.0
38	1578.0
39	3525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.864507600793125	16.94646397884997	13.139458030403173	25.049570389953736
2	22.048909451421018	18.91606080634501	31.804362194315928	27.230667547918042
3	21.573033707865168	20.60806345009914	27.547918043621944	30.270984798413746
4	15.888962326503636	16.07402511566424	30.627891606080638	37.40912095175148
5	16.840713813615334	34.47455386649042	30.38995373430271	18.29477858559154
6	34.05155320555188	30.58823529411765	17.078651685393258	18.28155981493721
7	31.89689358889623	28.83013879709187	17.79246530072703	21.480502313284862
8	32.769332452081954	27.111698612029084	18.083278255122273	22.035690680766688
9	29.7951090548579	13.02048909451421	17.461996034368806	39.72240581625909
10	22.220753469927295	22.855254461335093	26.23925974884336	28.684732319894252
11	37.316589557171184	21.79775280898876	16.523463317911435	24.36219431592862
12	25.657633840052874	25.908790482485127	23.040317250495704	25.39325842696629
13	34.71249173826834	16.642432253800397	23.648380700594846	24.996695307336417
14	24.78519497686715	20.092531394580305	22.80237937871778	32.31989424983476
15	31.421017845340383	23.23859881031064	18.506278916060808	26.834104428288168
16	28.076668869795107	23.727693324520818	22.30006609385327	25.8955717118308
17	24.771976206212823	26.31857237276933	21.83740912095175	27.072042300066094
18	26.186384666226044	20.92531394580304	25.340383344348975	27.547918043621944
19	26.68869795109055	25.155320555188364	23.489755452742894	24.666226040978188
20	28.010575016523465	20.91209517514871	24.679444811632518	26.39788499669531
21	30.429610046265697	20.978189028420356	23.304692663582287	25.287508261731663
22	29.49107732980833	21.136814276272307	22.035690680766688	27.336417713152677
23	27.73298083278255	22.02247191011236	22.74950429610046	27.49504296100463
24	25.76338400528751	22.65697290152016	24.63978849966953	26.939854593522806
25	26.411103767349637	21.73165895571712	23.410442828816922	28.446794448116325
26	27.455386649041642	21.83740912095175	23.278255122273627	27.42894910773298
27	27.640449438202246	21.044282881692002	23.397224058162593	27.918043621943163
28	25.76338400528751	21.86384666226041	24.49438202247191	27.878387309980173
29	25.499008592200926	22.141440846001323	26.186384666226044	26.17316589557171
30	24.33575677461996	23.357567746199603	26.754791804362192	25.551883674818242
31	25.155320555188364	23.450099140779905	24.282881692002643	27.111698612029084
32	25.39325842696629	23.595505617977526	25.604758757435558	25.40647719762062
33	25.181758096497024	22.577660277594184	25.86913417052214	26.371447455386647
34	25.499008592200926	22.683410442828816	25.049570389953736	26.768010575016522
35	24.653007270323858	22.65697290152016	25.089226701916722	27.60079312623926
36	23.648380700594846	23.027098479841374	25.82947785855915	27.49504296100463
37	24.33575677461996	21.321877065432915	26.913417052214143	27.42894910773298
38	23.56906807666887	19.947124917382684	28.354263053536023	28.129543952412426
39	24.904163912756115	20.237937871777927	29.147389292795772	25.710508922670193
40	23.331130204890947	20.105750165234635	28.5261070720423	28.03701255783212
41	20.60806345009914	21.07072042300066	30.971579643093193	27.349636483807004
42	21.86384666226041	20.647719762062128	28.750826173165894	28.737607402511568
43	19.418374091209518	21.136814276272307	30.60145406477198	28.843357567746196
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	4.0
6	8.0
7	12.0
8	13.0
9	14.0
10	18.5
11	23.0
12	23.0
13	42.5
14	62.0
15	90.5
16	119.0
17	102.0
18	85.0
19	85.0
20	96.5
21	108.0
22	75.0
23	42.0
24	40.0
25	38.0
26	38.0
27	36.0
28	34.0
29	43.0
30	52.0
31	57.0
32	62.0
33	62.0
34	76.5
35	91.0
36	102.5
37	114.0
38	128.0
39	142.0
40	142.0
41	188.0
42	234.0
43	298.0
44	362.0
45	406.0
46	450.0
47	450.0
48	562.0
49	674.0
50	657.5
51	641.0
52	597.0
53	553.0
54	553.0
55	503.5
56	454.0
57	472.5
58	491.0
59	463.5
60	436.0
61	436.0
62	453.5
63	471.0
64	477.5
65	484.0
66	442.0
67	400.0
68	400.0
69	366.0
70	332.0
71	284.0
72	236.0
73	177.0
74	118.0
75	118.0
76	103.5
77	89.0
78	72.0
79	55.0
80	43.5
81	32.0
82	32.0
83	28.0
84	24.0
85	17.5
86	11.0
87	11.0
88	11.0
89	11.0
90	7.0
91	3.0
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7565.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.12888301387972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.01242236024845	79.1804362194316
2	4.114906832298137	7.005948446794448
3	1.1335403726708075	2.894910773298083
4	0.6832298136645962	2.32650363516193
5	0.32608695652173914	1.3879709187045606
6	0.20186335403726707	1.0310641110376735
7	0.10869565217391304	0.6477197620621282
8	0.062111801242236024	0.4230006609385327
9	0.09316770186335403	0.713813615333774
>10	0.2639751552795031	4.388631857237277
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.6477197620621282	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.6477197620621282	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.5155320555188367	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	23	0.3040317250495704	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	18	0.23793787177792464	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.21150033046926636	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	14	0.18506278916060806	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	14	0.18506278916060806	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	14	0.18506278916060806	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	14	0.18506278916060806	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	14	0.18506278916060806	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	13	0.17184401850627892	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	12	0.15862524785194976	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.14540647719762062	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11	0.14540647719762062	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11	0.14540647719762062	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	10	0.13218770654329146	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	9	0.11896893588896232	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	9	0.11896893588896232	No Hit
ACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCCATTCG	9	0.11896893588896232	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	9	0.11896893588896232	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	9	0.11896893588896232	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	9	0.11896893588896232	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	8	0.10575016523463318	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.10575016523463318	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	8	0.10575016523463318	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	8	0.10575016523463318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.026437541308658295	0.0
19	0.0	0.0	0.0	0.026437541308658295	0.0
20	0.0	0.0	0.0	0.026437541308658295	0.0
21	0.0	0.0	0.0	0.03965631196298744	0.0
22	0.0	0.0	0.0	0.03965631196298744	0.0
23	0.0	0.0	0.0	0.03965631196298744	0.0
24	0.0	0.0	0.0	0.07931262392597488	0.0
25	0.0	0.0	0.0	0.09253139458030403	0.0
26	0.0	0.0	0.0	0.11896893588896233	0.0
27	0.0	0.0	0.0	0.2511566424322538	0.0
28	0.0	0.0	0.0	0.43621943159286186	0.0
29	0.0	0.0	0.0	0.7270323859881032	0.0
30	0.0	0.0	0.0	1.2161269001982815	0.0
31	0.0	0.0	0.0	2.4058162590879046	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE