FastQCFastQC Report
Fri 10 Feb 2017
ERR1631496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336486
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.6148844231260736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16110.47877177653750824No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14360.42676366921654985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.21308464542358374No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4660.13849016006609488No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4580.13611264658856534No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3780.11233751181327009No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3560.10579934975006389No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC3540.10520497138068151No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3370.10015275524093126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCT250.005490889329.5999984
GGTTCCG652.6734924E-622.769238
TAAGATT502.6966023E-422.1999994
GTATTAA450.00382026920.5555571
GAGTATA450.00382026920.5555571
ATACACA2100.019.3809537
GGTATCA10050.019.328361
GCAGTCG2100.018.59
GTTCCGG801.6111386E-518.59
CCGAGGA500.007024798518.49999826
CAGTCGG2250.018.08888810
AAGACGG1351.1423253E-917.8148165
TATACAC2300.017.69565236
AGTCGGT2300.017.69565211
TCTAGAT1054.7723734E-717.6190472
GGAGTAT752.0617963E-417.26666618
TCAAGGG752.0617963E-417.2666663
CGCAAGA1401.8535502E-917.1785722
CCCACGG650.001576796217.07692315
TCGGTGA2300.016.89130413
Produced by FastQC (version 0.11.5)