##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336486
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02764453795997	34.0	33.0	34.0	31.0	34.0
2	33.159394447317275	34.0	33.0	34.0	31.0	34.0
3	33.23329648187443	34.0	33.0	34.0	31.0	34.0
4	36.455623710941914	37.0	37.0	37.0	35.0	37.0
5	36.44950755752097	37.0	37.0	37.0	35.0	37.0
6	36.5063033826073	37.0	37.0	37.0	35.0	37.0
7	36.52262798452239	37.0	37.0	37.0	35.0	37.0
8	36.52914534334266	37.0	37.0	37.0	35.0	37.0
9	38.3786903467009	39.0	39.0	39.0	37.0	39.0
10	38.34307519480751	39.0	39.0	39.0	37.0	39.0
11	38.394833663213326	39.0	39.0	39.0	37.0	39.0
12	38.36668390363938	39.0	39.0	39.0	37.0	39.0
13	38.38509180173915	39.0	39.0	39.0	37.0	39.0
14	39.91698317314836	41.0	40.0	41.0	38.0	41.0
15	39.889873575720834	41.0	40.0	41.0	38.0	41.0
16	39.84729230933828	41.0	40.0	41.0	38.0	41.0
17	39.852763562228446	41.0	40.0	41.0	38.0	41.0
18	39.82474159400391	41.0	40.0	41.0	38.0	41.0
19	39.84047776133331	41.0	40.0	41.0	38.0	41.0
20	39.805097388895824	41.0	40.0	41.0	38.0	41.0
21	39.77776489957977	41.0	40.0	41.0	38.0	41.0
22	39.70367860772811	41.0	40.0	41.0	37.0	41.0
23	39.637384616299045	41.0	40.0	41.0	37.0	41.0
24	39.59307370886159	41.0	40.0	41.0	37.0	41.0
25	39.59726110447389	41.0	40.0	41.0	37.0	41.0
26	39.49006199366393	41.0	40.0	41.0	36.0	41.0
27	39.435138460441145	41.0	39.0	41.0	36.0	41.0
28	39.35555714056454	41.0	39.0	41.0	36.0	41.0
29	39.300265092752745	41.0	39.0	41.0	35.0	41.0
30	39.21890063776799	41.0	39.0	41.0	35.0	41.0
31	39.15306134579151	41.0	39.0	41.0	35.0	41.0
32	39.06309920769363	41.0	39.0	41.0	35.0	41.0
33	38.9669436469868	41.0	39.0	41.0	35.0	41.0
34	38.89865254423661	41.0	39.0	41.0	35.0	41.0
35	38.776828753648	41.0	38.0	41.0	35.0	41.0
36	38.698332174295516	40.0	38.0	41.0	35.0	41.0
37	38.614857676099454	40.0	38.0	41.0	35.0	41.0
38	38.52968028387511	40.0	38.0	41.0	35.0	41.0
39	38.41789554394536	40.0	38.0	41.0	35.0	41.0
40	38.277738152553155	40.0	38.0	41.0	35.0	41.0
41	38.15253234904275	40.0	37.0	41.0	35.0	41.0
42	37.98818078612483	40.0	37.0	41.0	34.0	41.0
43	37.162847785643386	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	3.0
17	5.0
18	6.0
19	12.0
20	27.0
21	35.0
22	75.0
23	155.0
24	195.0
25	371.0
26	518.0
27	734.0
28	1148.0
29	1656.0
30	2173.0
31	2770.0
32	3368.0
33	4495.0
34	6817.0
35	9726.0
36	14961.0
37	29760.0
38	61051.0
39	196423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61459317772508	17.519599626730383	12.659962078660033	26.205845116884507
2	19.23319246565979	19.93010110376063	33.947028999720644	26.88967743085894
3	20.31109763853474	22.12840950292137	28.61575221554537	28.944740642998518
4	14.537900536723667	15.471668955023388	32.92440101519826	37.066029493054685
5	16.022657703440856	35.840124106203525	31.633411196899726	16.503806993455893
6	34.54705396361216	33.29945376627854	16.236633916418512	15.916858353690793
7	30.276148190415054	29.95934451953425	20.19162758628888	19.57287970376182
8	29.294829502564745	31.202486879097496	19.29560219444494	20.207081423892824
9	28.140546709224157	13.767288980819409	19.083409116575428	39.008755193381
10	19.23319246565979	25.32467918427513	29.76765749540843	25.674470854656654
11	36.98103338623301	21.74206356282282	19.599626730384028	21.677276320560143
12	23.81525531522857	25.525876262311066	26.918207592589287	23.74066082987108
13	30.890735424356436	18.305367831053893	24.740999625541626	26.062897119048046
14	23.69073304684296	20.063539047686977	24.312452821216933	31.933275084253133
15	26.955059051491	26.09172446996309	21.57444886265699	25.378767615888925
16	25.893202094589373	24.710983517887815	23.439608185778905	25.95620620174391
17	24.45777833253092	25.94699333701848	24.346035199087034	25.249193131363562
18	24.831047948503056	23.06425824551393	26.475098518214722	25.629595287768286
19	25.181433997253972	24.670862977954506	25.67239053036382	24.475312494427705
20	25.78027020440672	23.56323888661044	25.77194890723537	24.884542001747473
21	27.992249306063254	23.477351212234684	24.715144166473493	23.81525531522857
22	26.79279375664961	23.455359212567537	24.4922522779551	25.25959475282776
23	25.313980373626244	23.639616507076074	25.078012160981437	25.968390958316245
24	24.896132379950426	24.610533573462195	25.507153343675515	24.98618070291186
25	24.846501786107	23.69340774950518	25.59274382886658	25.867346635521237
26	25.58442253169523	24.60993919509281	25.481892262976764	24.323746010235194
27	26.127981550495416	23.559672616394145	24.78706394916876	25.52528188394168
28	24.249448714062396	23.976629042515885	26.410014086767355	25.363908156654364
29	24.23993866015228	24.143649364312335	26.592785435352432	25.023626540182946
30	23.83754450408041	24.715441355658186	26.67867310972819	24.768341030533218
31	24.69196341006758	24.581111844177766	25.297337779283534	25.429586966471117
32	23.9929744476739	24.043793798256093	26.055764578615452	25.907467175454553
33	24.118685472798273	23.667552290437047	26.47658446413818	25.737177772626495
34	25.216502321047535	23.699351533199003	26.038824795088054	25.045321350665407
35	24.915449676955355	23.645263101585208	26.58921916513614	24.850068056323295
36	23.512419536028244	24.282436713563122	26.24685722437189	25.958286526036744
37	23.87320720624335	23.22860386464816	27.045107374452428	25.853081554656065
38	23.559969805578834	22.34119695916026	28.06595222386667	26.03288101139423
39	23.702620614230607	22.397960093436282	28.060008440172844	25.839410852160267
40	23.171246352002758	22.497221281123135	28.686780430686564	25.64475193618754
41	21.662119672140893	22.95192073370066	29.67671760489292	25.709241989265525
42	22.400040417729116	23.230684188940995	29.06480507361376	25.304470319716128
43	21.236841948847797	22.48057868678043	29.44401847327972	26.83856089109205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	66.5
2	62.0
3	184.5
4	307.0
5	307.0
6	398.0
7	489.0
8	469.5
9	450.0
10	749.5
11	1049.0
12	1049.0
13	1598.0
14	2147.0
15	3179.5
16	4212.0
17	3738.5
18	3265.0
19	3265.0
20	3554.0
21	3843.0
22	3048.5
23	2254.0
24	2501.0
25	2748.0
26	2748.0
27	3035.5
28	3323.0
29	3984.0
30	4645.0
31	5388.0
32	6131.0
33	6131.0
34	7302.0
35	8473.0
36	9988.5
37	11504.0
38	12864.0
39	14224.0
40	14224.0
41	15313.5
42	16403.0
43	17569.5
44	18736.0
45	19903.5
46	21071.0
47	21071.0
48	22794.0
49	24517.0
50	24930.0
51	25343.0
52	25450.5
53	25558.0
54	25558.0
55	24787.0
56	24016.0
57	23222.0
58	22428.0
59	21095.0
60	19762.0
61	19762.0
62	18421.0
63	17080.0
64	15662.0
65	14244.0
66	12489.0
67	10734.0
68	10734.0
69	9347.5
70	7961.0
71	7106.0
72	6251.0
73	5379.0
74	4507.0
75	4507.0
76	4045.5
77	3584.0
78	2901.5
79	2219.0
80	1641.0
81	1063.0
82	1063.0
83	864.0
84	665.0
85	586.0
86	507.0
87	460.0
88	413.0
89	413.0
90	299.0
91	185.0
92	107.0
93	29.0
94	18.0
95	7.0
96	7.0
97	6.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	336486.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.233476046302584
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17142867690167	55.21510017947941
2	4.809065699978518	5.889516186894599
3	1.5321017852984002	2.8144775391170103
4	0.7970650943508749	1.9522826544911283
5	0.4942667498130171	1.513283559257961
6	0.3362226140957845	1.2352847631875674
7	0.24405937756631413	1.046122283505769
8	0.17222266861763835	0.8436634122742772
9	0.15251302353674598	0.8405012316137864
>10	1.0696504585816313	12.973624167469369
>50	0.14111454256096498	5.986975382492175
>100	0.07834290121488022	7.95114906095671
>500	4.8660187089987725E-4	0.2136396431218273
>1k	0.0014598056126996315	1.5243799361384496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2069	0.6148844231260736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1611	0.47877177653750824	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1436	0.42676366921654985	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	717	0.21308464542358374	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	466	0.13849016006609488	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	458	0.13611264658856534	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	378	0.11233751181327009	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	356	0.10579934975006389	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	354	0.10520497138068151	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	337	0.10015275524093126	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.001188756738764763	0.0
12	0.0	0.0	0.0	0.002377513477529526	0.0
13	0.0	0.0	0.0	0.003269081031603098	0.0
14	0.0	0.0	0.0	0.0038634594009854793	0.0
15	0.0	0.0	0.0	0.005646594509132624	0.0
16	0.0	0.0	0.0	0.009212864725426912	0.0
17	0.0	0.0	0.0	0.013373513311103582	0.0
18	0.0	0.0	0.0	0.014859459234559535	0.0
19	0.0	0.0	0.0	0.018722918635545014	0.0
20	0.0	0.0	0.0	0.021991999667148113	0.0
21	0.0	0.0	0.0	0.027638594176280736	0.0
22	0.0	0.0	0.0	0.0353655129782517	0.0
23	0.0	0.0	0.0	0.04457837770367861	0.0
24	0.0	0.0	0.0	0.058249080199473384	0.0
25	0.0	0.0	0.0	0.06359848552391481	0.0
26	0.0	0.0	0.0	0.07875513394316554	0.0
27	0.0	0.0	0.0	0.12036161979993223	0.0
28	0.0	0.0	0.0	0.28975945507391093	0.0
29	0.0	0.0	0.0	0.529888316304393	0.0
30	0.0	0.0	0.0	0.8663064733748209	0.0
31	0.0	0.0	0.0	1.6184922998282247	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGCT	25	0.0054908893	29.599998	4
GGTTCCG	65	2.6734924E-6	22.76923	8
TAAGATT	50	2.6966023E-4	22.199999	4
GTATTAA	45	0.003820269	20.555557	1
GAGTATA	45	0.003820269	20.555557	1
ATACACA	210	0.0	19.38095	37
GGTATCA	1005	0.0	19.32836	1
GCAGTCG	210	0.0	18.5	9
GTTCCGG	80	1.6111386E-5	18.5	9
CCGAGGA	50	0.0070247985	18.499998	26
CAGTCGG	225	0.0	18.088888	10
AAGACGG	135	1.1423253E-9	17.814816	5
TATACAC	230	0.0	17.695652	36
AGTCGGT	230	0.0	17.695652	11
TCTAGAT	105	4.7723734E-7	17.619047	2
GGAGTAT	75	2.0617963E-4	17.266666	18
TCAAGGG	75	2.0617963E-4	17.266666	3
CGCAAGA	140	1.8535502E-9	17.178572	2
CCCACGG	65	0.0015767962	17.076923	15
TCGGTGA	230	0.0	16.891304	13
>>END_MODULE