FastQCFastQC Report
Fri 10 Feb 2017
ERR1631495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353775
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.230937742915695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8110.22924174969966787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6470.18288460179492616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACAC358.8572124E-426.4285723
TTGTACT400.001928560223.1254
TCTAATA400.001928560223.1252
TAACACA400.001928560223.1254
GGTATCA4650.022.279571
TCGTTTA909.431824E-820.55555530
CGTTTAT909.431824E-820.55555531
ATACCGC1001.2822056E-820.34999827
AGACTGC555.1334273E-420.1818186
TAATACT609.2195964E-418.54
ACCGCAG1103.8291546E-818.529
CTTTCGT801.6115217E-518.527
GGGTGCG500.007025494718.4999987
ACTTCAC500.007025494718.4999988
TATTATT500.007025494718.4999982
GTCTTAG500.007025494718.4999981
CTCTATG2350.018.1063841
CAGTCGG1258.518327E-917.7610
CGAGCCG1156.367736E-817.69565215
ATCGTTT1054.7741014E-717.61904729
Produced by FastQC (version 0.11.5)