##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631495.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	353775
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.062028125220834	34.0	33.0	34.0	31.0	34.0
2	33.208926577627025	34.0	33.0	34.0	31.0	34.0
3	33.29560031093209	34.0	34.0	34.0	31.0	34.0
4	36.498955550844464	37.0	37.0	37.0	35.0	37.0
5	36.48981132075472	37.0	37.0	37.0	35.0	37.0
6	36.54300614797541	37.0	37.0	37.0	35.0	37.0
7	36.555757190304575	37.0	37.0	37.0	35.0	37.0
8	36.55377853155254	37.0	37.0	37.0	35.0	37.0
9	38.39858384566462	39.0	39.0	39.0	37.0	39.0
10	38.36758956964172	39.0	39.0	39.0	37.0	39.0
11	38.415801003462654	39.0	39.0	39.0	37.0	39.0
12	38.38004663981344	39.0	39.0	39.0	37.0	39.0
13	38.410729983746734	39.0	39.0	39.0	37.0	39.0
14	39.964146703413185	41.0	40.0	41.0	38.0	41.0
15	39.95524839233976	41.0	40.0	41.0	38.0	41.0
16	39.905307045438484	41.0	40.0	41.0	38.0	41.0
17	39.900103172920645	41.0	40.0	41.0	38.0	41.0
18	39.87340541304501	41.0	40.0	41.0	38.0	41.0
19	39.88949756200975	41.0	40.0	41.0	38.0	41.0
20	39.844124090170304	41.0	40.0	41.0	38.0	41.0
21	39.82531552540456	41.0	40.0	41.0	38.0	41.0
22	39.7710578757685	41.0	40.0	41.0	38.0	41.0
23	39.71645820083386	41.0	40.0	41.0	37.0	41.0
24	39.68900289732174	41.0	40.0	41.0	37.0	41.0
25	39.68056533107201	41.0	40.0	41.0	37.0	41.0
26	39.57561444420889	41.0	40.0	41.0	37.0	41.0
27	39.5328471486114	41.0	40.0	41.0	37.0	41.0
28	39.4727128824818	41.0	40.0	41.0	36.0	41.0
29	39.41877747155678	41.0	39.0	41.0	36.0	41.0
30	39.35066921065649	41.0	39.0	41.0	36.0	41.0
31	39.30053847784609	41.0	39.0	41.0	35.0	41.0
32	39.23299272136245	41.0	39.0	41.0	35.0	41.0
33	39.15708854497915	41.0	39.0	41.0	35.0	41.0
34	39.10612960214826	41.0	39.0	41.0	35.0	41.0
35	39.01175888629779	41.0	39.0	41.0	35.0	41.0
36	38.94899017737262	41.0	39.0	41.0	35.0	41.0
37	38.882716415801006	41.0	39.0	41.0	35.0	41.0
38	38.80841495300685	40.0	38.0	41.0	35.0	41.0
39	38.72192495230019	40.0	38.0	41.0	35.0	41.0
40	38.626499894000425	40.0	38.0	41.0	35.0	41.0
41	38.531741926365626	40.0	38.0	41.0	35.0	41.0
42	38.40584834993993	40.0	38.0	41.0	35.0	41.0
43	37.60351635926789	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	0.0
17	1.0
18	4.0
19	9.0
20	17.0
21	31.0
22	67.0
23	126.0
24	181.0
25	305.0
26	463.0
27	632.0
28	904.0
29	1335.0
30	1802.0
31	2384.0
32	3009.0
33	4075.0
34	6184.0
35	9138.0
36	14915.0
37	28847.0
38	66577.0
39	212767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.90495371351848	17.651049395802417	12.14811674086637	27.295880149812735
2	20.076602360257226	19.441170235319056	32.84912727015759	27.63310013426613
3	20.497774008903967	21.069606388241112	28.08084234329729	30.35177725955763
4	15.257154971380116	15.377853155254046	32.54610981556073	36.818882057805105
5	17.121616846865944	33.97696275881563	31.747014345275954	17.15440604904247
6	35.51918592325631	32.867500529997876	15.5124019503922	16.100911596353615
7	31.31594940286905	28.425694297222808	20.221609780227546	20.036746519680587
8	28.75556497774009	30.992580029679885	19.034131863472545	21.217723129107483
9	27.55762843615292	14.092290297505478	19.072291710833156	39.27778955550845
10	19.627164158010036	24.39318776058229	29.965938802911456	26.013709278496222
11	38.0812663416013	20.71683979930747	19.87251784326196	21.329376015829272
12	23.680870609850892	24.399123736838384	26.45297152144725	25.467034131863475
13	31.633948130874145	17.818952724189103	23.463500812663415	27.08359833227334
14	24.366334534661863	19.624902833722	22.91795632817469	33.09080630344145
15	27.523991237368385	25.716062469083457	20.278142887428448	26.48180340611971
16	27.109603561585754	23.972016111935552	22.61380821143382	26.304572115044873
17	25.692318564059075	24.835276658893367	23.524839233976397	25.94756554307116
18	26.012861281888206	22.897321744046355	24.70807716769133	26.38173980637411
19	26.29948413539679	24.005370645184083	24.38866511200622	25.306480107412906
20	26.42753162320684	23.15483004734648	24.262313617412197	26.155324712034485
21	27.65345205285846	23.377287824182037	23.82305137446117	25.14620874849834
22	27.318493392693092	22.978446752879655	23.383506465974136	26.319553388453116
23	25.93823758038301	23.158504699314538	24.098367606529575	26.804890113772878
24	25.926365627870823	23.884107130238146	23.73175040633171	26.457776835559326
25	26.26302028125221	22.910324358702567	23.95929616281535	26.867359197229877
26	26.377499823334038	23.9686241255035	24.062186417920994	25.591689633241465
27	26.62681082609003	23.128542152498056	23.74814500741997	26.496502013991947
28	25.427178291286832	23.437212917814996	24.91385767790262	26.221751112995552
29	25.249381669139993	23.63790544837821	25.145360751890326	25.967352130591475
30	24.933361599886933	24.188537912515017	24.844039290509503	26.034061197088544
31	26.265846936612252	23.784043530492543	23.57939368242527	26.370715850469935
32	25.293477492756693	23.408381033142533	24.212281817539395	27.08585965656137
33	24.875697830542013	22.946505547311144	25.22789908840365	26.9498975337432
34	26.259628294820153	22.687583916331	24.620733517065933	26.432054271782913
35	25.904035050526463	22.86622853508586	25.358490566037734	25.87124584834994
36	24.795703483852733	23.231715073139707	25.08034767860929	26.892233764398277
37	25.29913080347679	21.898947070878382	25.960850823263375	26.841071302381458
38	24.440958236167056	21.74206769839587	27.00332132004805	26.813652745389017
39	24.463854144583422	21.426612960214825	27.342237297717475	26.767295597484274
40	24.14133276800226	21.557487103384922	27.8688431912939	26.43233693731892
41	22.93604692247898	21.844109956893508	28.742562363083877	26.477280757543635
42	23.425058299766803	22.195180552611124	28.523779238216378	25.855981909405696
43	22.4625821496714	21.546463147480743	28.78185287258851	27.209101830259346
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	36.0
2	45.0
3	80.0
4	115.0
5	115.0
6	150.5
7	186.0
8	174.5
9	163.0
10	278.0
11	393.0
12	393.0
13	673.0
14	953.0
15	1370.0
16	1787.0
17	1628.0
18	1469.0
19	1469.0
20	1683.5
21	1898.0
22	1548.5
23	1199.0
24	1358.0
25	1517.0
26	1517.0
27	1820.5
28	2124.0
29	2887.5
30	3651.0
31	4362.0
32	5073.0
33	5073.0
34	6509.5
35	7946.0
36	9617.0
37	11288.0
38	13181.5
39	15075.0
40	15075.0
41	16532.0
42	17989.0
43	19472.0
44	20955.0
45	22483.0
46	24011.0
47	24011.0
48	25502.5
49	26994.0
50	27401.5
51	27809.0
52	28050.0
53	28291.0
54	28291.0
55	27641.0
56	26991.0
57	25787.5
58	24584.0
59	23993.5
60	23403.0
61	23403.0
62	21584.5
63	19766.0
64	18246.0
65	16726.0
66	14887.5
67	13049.0
68	13049.0
69	11114.0
70	9179.0
71	8179.5
72	7180.0
73	5545.0
74	3910.0
75	3910.0
76	3217.5
77	2525.0
78	2192.0
79	1859.0
80	1645.5
81	1432.0
82	1432.0
83	1136.0
84	840.0
85	758.0
86	676.0
87	567.0
88	458.0
89	458.0
90	329.0
91	200.0
92	113.5
93	27.0
94	19.5
95	12.0
96	12.0
97	6.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	353775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.48240069001822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.34129559186921	55.197762286465114
2	5.88911286236831	7.3593181915047365
3	1.820219913999245	3.41195130031354
4	0.9014285291767152	2.2529367421373316
5	0.5626059995059501	1.757648674586948
6	0.4031256943637768	1.5112956698207591
7	0.30869907619520104	1.3501781560026909
8	0.2269423343931686	1.134392149686565
9	0.18401165134144068	1.0347740757673043
>10	1.1950803595995614	14.25324095725697
>50	0.11225551573643662	4.857473746224779
>100	0.05386454182513234	5.235607219219634
>500	0.0013579296258436724	0.643420831013649
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.230937742915695	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.22924174969966787	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	647	0.18288460179492616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	2.8266553600452265E-4
9	0.0	0.0	0.0	0.0	2.8266553600452265E-4
10	0.0	0.0	0.0	0.0	2.8266553600452265E-4
11	0.0	0.0	0.0	0.0	2.8266553600452265E-4
12	0.0	0.0	0.0	0.0	2.8266553600452265E-4
13	0.0	0.0	0.0	2.8266553600452265E-4	2.8266553600452265E-4
14	0.0	0.0	0.0	2.8266553600452265E-4	2.8266553600452265E-4
15	0.0	0.0	0.0	5.653310720090453E-4	2.8266553600452265E-4
16	0.0	0.0	0.0	8.479966080135679E-4	2.8266553600452265E-4
17	0.0	0.0	0.0	0.0016959932160271359	2.8266553600452265E-4
18	0.0	0.0	0.0	0.002261324288036181	2.8266553600452265E-4
19	0.0	0.0	0.0	0.003109320896049749	2.8266553600452265E-4
20	0.0	0.0	0.0	0.003957317504063317	5.653310720090453E-4
21	0.0	0.0	0.0	0.00537064518408593	8.479966080135679E-4
22	0.0	0.0	0.0	0.00537064518408593	8.479966080135679E-4
23	0.0	0.0	0.0	0.007066638400113066	8.479966080135679E-4
24	0.0	0.0	0.0	0.011589286976185428	8.479966080135679E-4
25	0.0	0.0	0.0	0.01695993216027136	8.479966080135679E-4
26	0.0	0.0	0.0	0.023743905024379904	8.479966080135679E-4
27	0.0	0.0	0.0	0.06303441452900856	8.479966080135679E-4
28	0.0	0.0	0.0	0.19871387181117942	8.479966080135679E-4
29	0.0	0.0	0.0	0.3584198996537347	8.479966080135679E-4
30	0.0	0.0	0.0	0.5732457070171719	8.479966080135679E-4
31	0.0	0.0	0.0	1.1527100558264434	8.479966080135679E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACAC	35	8.8572124E-4	26.428572	3
TTGTACT	40	0.0019285602	23.125	4
TCTAATA	40	0.0019285602	23.125	2
TAACACA	40	0.0019285602	23.125	4
GGTATCA	465	0.0	22.27957	1
TCGTTTA	90	9.431824E-8	20.555555	30
CGTTTAT	90	9.431824E-8	20.555555	31
ATACCGC	100	1.2822056E-8	20.349998	27
AGACTGC	55	5.1334273E-4	20.181818	6
TAATACT	60	9.2195964E-4	18.5	4
ACCGCAG	110	3.8291546E-8	18.5	29
CTTTCGT	80	1.6115217E-5	18.5	27
GGGTGCG	50	0.0070254947	18.499998	7
ACTTCAC	50	0.0070254947	18.499998	8
TATTATT	50	0.0070254947	18.499998	2
GTCTTAG	50	0.0070254947	18.499998	1
CTCTATG	235	0.0	18.106384	1
CAGTCGG	125	8.518327E-9	17.76	10
CGAGCCG	115	6.367736E-8	17.695652	15
ATCGTTT	105	4.7741014E-7	17.619047	29
>>END_MODULE