FastQCFastQC Report
Fri 10 Feb 2017
ERR1631494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47980
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20554.2830345977490625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15983.3305543976656944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13822.8803668195081285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5781.204668611921634No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3840.8003334722801168No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2130.44393497290537726No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.4230929553980825No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1960.40850354314297627No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1680.35014589412255104No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1670.3480616923718216No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.32930387661525634No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1540.32096706961233845No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1510.3147144643601501No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1470.30637765735723216No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.30012505210504375No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1390.28970404335139643No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1370.28553563984993746No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1350.28136723634847854No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1310.27303042934556065No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1260.26260942059191333No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.26052521884118385No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.25427261358899544No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1190.24802000833680699No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1150.23968320133388915No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG1100.22926219258024177No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1080.22509378907878283No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.20216756982075865No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA890.18549395581492287No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.18132555231346395No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT850.177157148812005No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT850.177157148812005No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.1709045435598166No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.1625677365568987No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA750.1563151313047103No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA740.15423092955398082No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA700.14589412255106293No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.14380992080033347No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.13755731554814507No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT650.13547311379741558No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT650.13547311379741558No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT650.13547311379741558No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG620.12922050854522718No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT610.12713630679449772No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.12296790329303876No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT580.12088370154230929No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC580.12088370154230929No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA570.11879949979157983No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT560.11671529804085036No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA560.11671529804085036No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC540.11254689453939141No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA540.11254689453939141No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT530.11046269278866194No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA530.11046269278866194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG510.10629428928720301No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT510.10629428928720301No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.10629428928720301No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC490.10212588578574407No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAA480.1000416840350146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCTG250.005444624529.5999985
GAGCTGA358.745537E-426.4285726
AGCTGAT400.00190447423.1257
CATGGGT502.6501503E-422.1999994
AGGGGAA500.00693941618.49999810
GAGAGGG1054.5650995E-717.6190477
CAGTCGG2350.017.31914910
GGCAGTC2350.017.3191498
GGAACAG650.001550289817.07692322
GGGCAGT2400.016.9583347
GCAGTCG2400.016.9583349
AGTCGGT2400.016.95833411
TGGGCAG2450.016.6122446
GCCTTAT1452.746674E-916.58620626
CGCCTTA1452.746674E-916.58620625
TTCCTCG1504.305548E-916.03333320
GGTATCA9300.015.9139791
TCGCCTT1403.23962E-815.85714324
GGTGATT2600.015.65384615
TCCTCCG956.836975E-515.57894821
Produced by FastQC (version 0.11.5)