##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631494.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47980
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84679032930388	34.0	33.0	34.0	31.0	34.0
2	32.90206335973322	34.0	33.0	34.0	31.0	34.0
3	32.888328470195916	34.0	33.0	34.0	31.0	34.0
4	36.27392663609837	37.0	37.0	37.0	35.0	37.0
5	36.30339724885369	37.0	37.0	37.0	35.0	37.0
6	36.41900791996665	37.0	37.0	37.0	35.0	37.0
7	36.41825760733639	37.0	37.0	37.0	35.0	37.0
8	36.46283868278449	37.0	37.0	37.0	35.0	37.0
9	38.23795331388078	39.0	39.0	39.0	37.0	39.0
10	38.20654439349729	39.0	39.0	39.0	37.0	39.0
11	38.28070029178824	39.0	39.0	39.0	37.0	39.0
12	38.25896206752814	39.0	39.0	39.0	37.0	39.0
13	38.28845352230096	39.0	39.0	39.0	37.0	39.0
14	39.554710295956646	41.0	39.0	41.0	37.0	41.0
15	39.47357232180075	41.0	39.0	41.0	37.0	41.0
16	39.348290954564405	40.0	39.0	41.0	37.0	41.0
17	39.43611921634014	40.0	39.0	41.0	37.0	41.0
18	39.63309712380158	41.0	39.0	41.0	37.0	41.0
19	39.7074406002501	41.0	40.0	41.0	38.0	41.0
20	39.75168820341809	41.0	40.0	41.0	38.0	41.0
21	39.74872863693206	41.0	40.0	41.0	37.0	41.0
22	39.641954981242186	41.0	40.0	41.0	37.0	41.0
23	39.54833263859942	41.0	40.0	41.0	36.0	41.0
24	39.48265944143393	41.0	40.0	41.0	36.0	41.0
25	39.484785327219676	41.0	40.0	41.0	36.0	41.0
26	39.25368903709879	41.0	39.0	41.0	35.0	41.0
27	39.07223843268029	41.0	39.0	41.0	35.0	41.0
28	38.908357649020424	41.0	38.0	41.0	35.0	41.0
29	38.659920800333474	40.0	38.0	41.0	35.0	41.0
30	38.330762817840764	40.0	37.0	41.0	35.0	41.0
31	37.951813255523135	40.0	35.0	41.0	35.0	41.0
32	37.64095456440183	39.0	35.0	41.0	35.0	41.0
33	37.37757398916215	39.0	35.0	41.0	35.0	41.0
34	37.166194247603165	39.0	35.0	41.0	34.0	41.0
35	36.90664860358483	39.0	35.0	41.0	33.0	41.0
36	36.65164651938308	39.0	35.0	41.0	33.0	41.0
37	36.34806169237182	39.0	35.0	41.0	33.0	41.0
38	36.03551479783243	38.0	35.0	41.0	32.0	41.0
39	35.65712796998749	38.0	35.0	41.0	30.0	41.0
40	35.266527719883285	38.0	35.0	41.0	26.0	41.0
41	34.7897040433514	37.0	35.0	41.0	21.0	41.0
42	34.27184243434765	37.0	35.0	41.0	16.0	41.0
43	33.28820341809087	35.0	34.0	40.0	11.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	1.0
13	0.0
14	1.0
15	1.0
16	1.0
17	2.0
18	1.0
19	1.0
20	5.0
21	17.0
22	18.0
23	33.0
24	66.0
25	86.0
26	143.0
27	244.0
28	373.0
29	525.0
30	691.0
31	789.0
32	959.0
33	1111.0
34	1544.0
35	2005.0
36	3304.0
37	9620.0
38	9523.0
39	16913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.682784493538975	13.689037098791163	16.84451854939558	20.78365985827428
2	18.299291371404752	23.851604835348063	34.39141308878699	23.45769070446019
3	19.906210921217173	26.998749478949563	31.021258857857443	22.073780741975824
4	11.89245518966236	19.5122967903293	30.67319716548562	37.922050854522716
5	11.517298874531054	43.670279283034596	28.528553563984993	16.283868278449354
6	25.095873280533553	32.26552730304294	22.455189662359317	20.183409754064193
7	27.955398082534387	35.34389328887036	17.740725302209253	18.95998332638599
8	37.55731554814506	27.899124635264695	16.65694039182993	17.886619424760315
9	33.02417674030846	12.057107127969987	17.40308461859108	37.51563151313047
10	21.64860358482701	30.837849103793246	20.933722384326803	26.579824927052936
11	30.598165902459357	29.355981659024593	14.431012922050854	25.614839516465192
12	24.320550229262192	34.795748228428515	24.120466861192163	16.76323468111713
13	35.74406002501042	19.345560650270947	25.5585660691955	19.351813255523133
14	23.755731554814506	23.555648186744477	28.405585660691955	24.28303459774906
15	31.21508962067528	26.263026260942056	23.18882867861609	19.333055439766568
16	19.716548561900794	27.265527303042937	29.456023343059606	23.561900791996663
17	16.33180491871613	33.455606502709465	28.94747811588162	21.26511046269279
18	19.464360150062525	23.328470195914964	34.31846602751146	22.888703626511045
19	18.807836598582742	31.019174656106713	36.07127969987495	14.101709045435598
20	21.765318882867863	23.928720300125054	38.06169237182159	16.244268445185494
21	25.604418507711546	22.544810337640683	35.13130471029596	16.719466444351813
22	23.19924968736974	24.262192580241766	31.463109629012088	21.075448103376406
23	19.126719466444353	27.56773655689871	32.169654022509384	21.13588995414756
24	17.36973739057941	27.719883284701957	36.598582742809505	18.311796581909128
25	17.109212171738225	27.338474364318465	33.422259274697794	22.13005418924552
26	20.97749062109212	25.935806586077533	33.30554397665694	19.781158816173406
27	18.46602751146311	26.542309295539805	32.10504376823676	22.886619424760315
28	16.77365568987078	27.340558566069195	36.015006252605254	19.870779491454773
29	17.150896206752815	28.368070029178828	36.96748645268862	17.51354731137974
30	16.13588995414756	29.68945393914131	38.955814922884535	15.218841183826596
31	16.91954981242184	31.55273030429346	34.04960400166736	17.47811588161734
32	15.871196331804919	30.746144226761153	38.00125052105044	15.381408920383494
33	17.148812005002085	29.037098791162986	37.45102125885786	16.363067944977075
34	17.922050854522716	30.51688203418091	32.263443101292204	19.29762401000417
35	16.277615673197165	32.13630679449771	32.69904126719466	18.887036265110464
36	14.026677782409338	31.93205502292622	34.05377240516882	19.987494789495624
37	14.729053772405168	32.22592746977907	34.44768653605669	18.597332221759068
38	16.07544810337641	28.388912046686123	36.01917465610671	19.51646519383076
39	16.88411838265944	28.380575239683203	35.90245935806586	18.832847019591497
40	15.998332638599416	27.817840766986247	33.732805335556485	22.451021258857857
41	12.459358065860775	29.583159649854107	36.49645685702376	21.46102542726136
42	13.130471029595665	28.66819508128387	35.24802000833681	22.95331388078366
43	11.84660275114631	31.117132138390996	37.250937890787824	19.785327219674866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	6.5
2	10.0
3	72.0
4	134.0
5	134.0
6	196.5
7	259.0
8	272.5
9	286.0
10	446.5
11	607.0
12	607.0
13	1093.5
14	1580.0
15	2643.0
16	3706.0
17	3228.0
18	2750.0
19	2750.0
20	3003.0
21	3256.0
22	2198.0
23	1140.0
24	999.5
25	859.0
26	859.0
27	769.0
28	679.0
29	740.0
30	801.0
31	801.5
32	802.0
33	802.0
34	761.5
35	721.0
36	667.5
37	614.0
38	736.0
39	858.0
40	858.0
41	1011.5
42	1165.0
43	1518.5
44	1872.0
45	2432.5
46	2993.0
47	2993.0
48	4248.0
49	5503.0
50	5005.0
51	4507.0
52	3564.0
53	2621.0
54	2621.0
55	2019.0
56	1417.0
57	1377.0
58	1337.0
59	1274.5
60	1212.0
61	1212.0
62	1248.5
63	1285.0
64	1231.0
65	1177.0
66	1156.0
67	1135.0
68	1135.0
69	1015.5
70	896.0
71	787.5
72	679.0
73	580.0
74	481.0
75	481.0
76	383.5
77	286.0
78	231.0
79	176.0
80	137.0
81	98.0
82	98.0
83	71.0
84	44.0
85	30.5
86	17.0
87	13.0
88	9.0
89	9.0
90	6.5
91	4.0
92	2.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	47980.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.91663192997082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.48533151163231	48.59316381825761
2	5.639682720406847	6.1942476031679865
3	1.9507381684314393	3.2138390996248436
4	1.0512732931041027	2.3092955398082533
5	0.5730767771072907	1.5735723218007505
6	0.41367793844168654	1.3630679449770737
7	0.2960264146646932	1.137974155898291
8	0.212531784887472	0.9337223843268029
9	0.14421799688792744	0.712796998749479
>10	1.0209116095487496	10.93580658607753
>50	0.1138563133325743	4.139224676948729
>100	0.08349462977722115	7.194664443518133
>500	0.0037952104444191432	1.204668611921634
>1k	0.011385631333257429	10.493955814922884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2055	4.2830345977490625	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1598	3.3305543976656944	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1382	2.8803668195081285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	1.204668611921634	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	384	0.8003334722801168	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	213	0.44393497290537726	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.4230929553980825	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	196	0.40850354314297627	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	168	0.35014589412255104	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	167	0.3480616923718216	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.32930387661525634	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	154	0.32096706961233845	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	151	0.3147144643601501	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	147	0.30637765735723216	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	144	0.30012505210504375	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	139	0.28970404335139643	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	137	0.28553563984993746	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	135	0.28136723634847854	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	131	0.27303042934556065	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	126	0.26260942059191333	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	125	0.26052521884118385	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122	0.25427261358899544	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	119	0.24802000833680699	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	115	0.23968320133388915	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	110	0.22926219258024177	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	108	0.22509378907878283	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97	0.20216756982075865	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	89	0.18549395581492287	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	87	0.18132555231346395	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	85	0.177157148812005	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	85	0.177157148812005	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	82	0.1709045435598166	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.1625677365568987	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA	75	0.1563151313047103	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	74	0.15423092955398082	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	70	0.14589412255106293	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.14380992080033347	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	66	0.13755731554814507	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	65	0.13547311379741558	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	65	0.13547311379741558	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	65	0.13547311379741558	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	62	0.12922050854522718	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	61	0.12713630679449772	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.12296790329303876	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	58	0.12088370154230929	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	58	0.12088370154230929	No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA	57	0.11879949979157983	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	56	0.11671529804085036	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAA	56	0.11671529804085036	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	54	0.11254689453939141	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	54	0.11254689453939141	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	53	0.11046269278866194	No Hit
GAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAA	53	0.11046269278866194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	51	0.10629428928720301	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	51	0.10629428928720301	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.10629428928720301	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	49	0.10212588578574407	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAA	48	0.1000416840350146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0020842017507294707	0.0
14	0.0	0.0	0.0	0.008336807002917883	0.0
15	0.0	0.0	0.0	0.012505210504376824	0.0
16	0.0	0.0	0.0	0.016673614005835766	0.0
17	0.0	0.0	0.0	0.027094622759483118	0.0
18	0.0	0.0	0.0	0.02917882451021259	0.0
19	0.0	0.0	0.0	0.03126302626094206	0.0
20	0.0	0.0	0.0	0.03751563151313047	0.0
21	0.0	0.0	0.0	0.06252605252188412	0.0
22	0.0	0.0	0.0	0.08336807002917883	0.0
23	0.0	0.0	0.0	0.12505210504376824	0.0
24	0.0	0.0	0.0	0.1709045435598166	0.0
25	0.0	0.0	0.0	0.1834097540641934	0.0
26	0.0	0.0	0.0	0.20842017507294705	0.0
27	0.0	0.0	0.0	0.26469362234264276	0.0
28	0.0	0.0	0.0	0.39391413088787	0.0
29	0.0	0.0	0.0	0.7294706127553147	0.0
30	0.0	0.0	0.0	1.208837015423093	0.0
31	0.0	0.0	0.0	1.9945810754481035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAGCTG	25	0.0054446245	29.599998	5
GAGCTGA	35	8.745537E-4	26.428572	6
AGCTGAT	40	0.001904474	23.125	7
CATGGGT	50	2.6501503E-4	22.199999	4
AGGGGAA	50	0.006939416	18.499998	10
GAGAGGG	105	4.5650995E-7	17.619047	7
CAGTCGG	235	0.0	17.319149	10
GGCAGTC	235	0.0	17.319149	8
GGAACAG	65	0.0015502898	17.076923	22
GGGCAGT	240	0.0	16.958334	7
GCAGTCG	240	0.0	16.958334	9
AGTCGGT	240	0.0	16.958334	11
TGGGCAG	245	0.0	16.612244	6
GCCTTAT	145	2.746674E-9	16.586206	26
CGCCTTA	145	2.746674E-9	16.586206	25
TTCCTCG	150	4.305548E-9	16.033333	20
GGTATCA	930	0.0	15.913979	1
TCGCCTT	140	3.23962E-8	15.857143	24
GGTGATT	260	0.0	15.653846	15
TCCTCCG	95	6.836975E-5	15.578948	21
>>END_MODULE