FastQCFastQC Report
Fri 10 Feb 2017
ERR1631493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371805
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.12667930770161778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4500.1210311857021826No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA3800.10220411237073197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTT200.001840334737.021
TAGACTA250.00549162229.5999985
GTATAAT250.00549162229.5999981
GGTATAG809.731593E-1025.4375021
GTTCTAG1200.024.6666661
TAGAACT1200.023.1249984
TCTAGAC1450.021.6896553
GTCTAGA1059.767973E-1021.1428571
ATATAGT450.003821013720.5555573
AGGCTAA450.003821013720.55555716
CTCTAGT1450.020.41379427
CTAGAAC1202.3646862E-1020.0416663
ACATGTA1403.6379788E-1219.8214288
TCTAGTT1500.019.73333428
CTAGACA1700.019.5882364
ACTGATC1254.0927262E-1019.248
GTATTTT1551.8189894E-1219.09677512
CCTCTAG2450.018.8775526
GTCCTAT1103.8307917E-818.51
CCTACAC609.220805E-418.53
Produced by FastQC (version 0.11.5)