FastQCFastQC Report
Fri 10 Feb 2017
ERR1631492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276961
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.49104386538176853No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9890.3570899873989479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9320.3365094724528002No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3470.12528839800549535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.11734504135961382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACTA303.5924886E-430.8333345
ATACACA2450.024.9183737
ATCGGCA451.3196723E-424.66666616
GCGTTAT400.001927507823.1250021
ATACCGT400.001927507823.1250026
CAATACA450.00381858920.5555554
TAACCAG555.129557E-420.1818185
GGTATCA8350.019.275451
CGGGTAA500.007021736418.49999824
AGATACC500.007021736418.4999984
GGGTAAA500.007021736418.49999811
TAACCCG500.007021736418.49999828
GATAGTG500.007021736418.4999987
CAGTCGG3550.018.23943710
CGGTGAT3500.017.97142814
AGTCGGT3750.017.76000211
GCAGTCG3650.017.7397259
GTGATTC3600.017.47222116
GGCAGTC3750.017.2666688
TCGGTGA3650.017.23287613
Produced by FastQC (version 0.11.5)