##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631492.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	276961
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05823563606428	34.0	33.0	34.0	31.0	34.0
2	33.198082040431686	34.0	33.0	34.0	31.0	34.0
3	33.26832297688122	34.0	34.0	34.0	31.0	34.0
4	36.51123082311228	37.0	37.0	37.0	35.0	37.0
5	36.51066034568044	37.0	37.0	37.0	35.0	37.0
6	36.56784890291413	37.0	37.0	37.0	35.0	37.0
7	36.56824968136308	37.0	37.0	37.0	35.0	37.0
8	36.57091792707276	37.0	37.0	37.0	35.0	37.0
9	38.41994721278447	39.0	39.0	39.0	37.0	39.0
10	38.37923389935767	39.0	39.0	39.0	37.0	39.0
11	38.44210556720982	39.0	39.0	39.0	37.0	39.0
12	38.39522170991584	39.0	39.0	39.0	37.0	39.0
13	38.422521582461066	39.0	39.0	39.0	37.0	39.0
14	39.95369745198783	41.0	40.0	41.0	38.0	41.0
15	39.94910113698319	41.0	40.0	41.0	38.0	41.0
16	39.91345351872646	41.0	40.0	41.0	38.0	41.0
17	39.89844779589906	41.0	40.0	41.0	38.0	41.0
18	39.91932077079444	41.0	40.0	41.0	38.0	41.0
19	39.944064326746364	41.0	40.0	41.0	38.0	41.0
20	39.935066670036576	41.0	40.0	41.0	38.0	41.0
21	39.909593769519894	41.0	40.0	41.0	38.0	41.0
22	39.854947086412885	41.0	40.0	41.0	38.0	41.0
23	39.80742053935391	41.0	40.0	41.0	38.0	41.0
24	39.77243727456212	41.0	40.0	41.0	38.0	41.0
25	39.74370037658732	41.0	40.0	41.0	37.0	41.0
26	39.65659063911525	41.0	40.0	41.0	37.0	41.0
27	39.59997616993006	41.0	40.0	41.0	37.0	41.0
28	39.53159109044233	41.0	40.0	41.0	37.0	41.0
29	39.47199425189828	41.0	39.0	41.0	36.0	41.0
30	39.37841428937648	41.0	39.0	41.0	36.0	41.0
31	39.29529067269399	41.0	39.0	41.0	35.0	41.0
32	39.212658099876876	41.0	39.0	41.0	35.0	41.0
33	39.14025801466632	41.0	39.0	41.0	35.0	41.0
34	39.06558324096172	41.0	39.0	41.0	35.0	41.0
35	38.997461736489974	41.0	39.0	41.0	35.0	41.0
36	38.932708937359415	41.0	39.0	41.0	35.0	41.0
37	38.87127429493683	41.0	39.0	41.0	35.0	41.0
38	38.79662118493218	41.0	39.0	41.0	35.0	41.0
39	38.7036044786089	40.0	38.0	41.0	35.0	41.0
40	38.60066579771159	40.0	38.0	41.0	35.0	41.0
41	38.500565061506855	40.0	38.0	41.0	35.0	41.0
42	38.370572029997	40.0	38.0	41.0	35.0	41.0
43	37.57041244074075	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	0.0
16	0.0
17	3.0
18	4.0
19	3.0
20	11.0
21	37.0
22	40.0
23	77.0
24	145.0
25	203.0
26	335.0
27	508.0
28	782.0
29	1075.0
30	1417.0
31	1775.0
32	2171.0
33	3083.0
34	4449.0
35	6887.0
36	11165.0
37	23360.0
38	51983.0
39	167446.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.305375125017605	16.85363643256632	13.87559981369218	25.9653886287239
2	19.49046977733327	19.587234303746737	34.38859622834984	26.53369969057015
3	19.698080235123356	21.288195810962556	29.14489765707085	29.86882629684324
4	14.794140691288666	16.500879185156034	32.6558612945505	36.049118829004804
5	16.20444755759836	35.01828777336881	31.90557515318041	16.871689515852413
6	33.61484107870783	33.399648325937584	16.807420539353917	16.178090056000663
7	30.48804705355628	29.941038629987617	19.41428576586595	20.156628550590156
8	29.298709926668376	31.747430143594222	18.817811894093392	20.13604803564401
9	28.080487866522724	14.597723145135957	18.30510432876831	39.01668465957301
10	19.172374449832287	25.656319842865965	29.12178971046465	26.049515996837098
11	36.638371467462925	21.986128010802965	19.37168048931077	22.003820032423338
12	23.940193745689825	25.673289741154893	26.391080332610006	23.995436180545276
13	32.014615776228425	18.43905820675113	23.955719397315868	25.59060661970458
14	24.23157050992739	19.667028931871275	23.865453980885395	32.235946577315936
15	27.5670581778662	25.45159787840165	21.07336411985803	25.907979823874122
16	25.635017204588372	25.058040662764792	23.22962438754915	26.077317745097684
17	24.083535226981418	26.217048609732057	24.104837865259007	25.59457829802752
18	25.144334400872324	23.558912626687512	25.55052877480945	25.746224197630713
19	25.591689804701744	25.06273446441918	25.176468889121573	24.169106841757504
20	26.417437834207703	23.320611927311067	25.41404746516658	24.847902773314654
21	28.11190023144053	23.235040312534977	24.377439422879032	24.27562003314546
22	27.631327154364694	23.189185480988296	23.35599597055181	25.8234913940952
23	25.66173576785179	23.75352486451161	24.166218348431727	26.41852101920487
24	25.075371622719445	24.141666155162643	25.16058217582981	25.622380046288107
25	25.08367604103105	23.433263167016293	24.84104260166594	26.64201819028672
26	25.926393968825934	23.628236466506113	24.82840544336567	25.616964121302278
27	25.89028780225375	23.521362213452434	24.173078520080445	26.415271464213376
28	24.844653218323156	23.708753217962094	25.561721686446827	25.88487187726792
29	24.712143587003226	23.995436180545276	25.989579760327263	25.30284047212423
30	24.470593332635282	24.202685576669637	26.148085831579177	25.178635259115907
31	25.046125627795973	24.083535226981418	24.932391203093577	25.937947942129036
32	23.948859225667153	23.547719715050132	26.84854546307964	25.654875596203077
33	24.390076581179297	22.565631984286597	27.29590086690906	25.748390567625044
34	24.89339654319561	22.34827286152202	26.423575882524975	26.33475471275739
35	24.03298659378035	22.504973624445316	27.044601947566626	26.417437834207703
36	22.91080693671672	21.967352804185428	27.925231350262315	27.19660890883554
37	22.886254743447633	21.20117994952358	28.615942316788285	27.296622990240504
38	22.238149053476842	20.15374005726438	30.390560403811367	27.21755048544741
39	22.183628741952838	20.003899465989797	31.35856672961175	26.453905062445614
40	21.36726831575565	20.720967934113467	31.17875802008225	26.733005730048635
41	19.94179685948563	21.116691519744656	32.61650557298681	26.3250060477829
42	19.704940406772074	21.504471748729966	31.926877791458004	26.86371005303996
43	18.459638721697278	21.766241456378335	32.250750105610535	27.52336971631385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	20.0
2	27.0
3	87.5
4	148.0
5	148.0
6	196.0
7	244.0
8	254.0
9	264.0
10	413.0
11	562.0
12	562.0
13	856.5
14	1151.0
15	1887.5
16	2624.0
17	2368.5
18	2113.0
19	2113.0
20	2349.0
21	2585.0
22	2096.0
23	1607.0
24	1684.0
25	1761.0
26	1761.0
27	2028.5
28	2296.0
29	2903.0
30	3510.0
31	4077.0
32	4644.0
33	4644.0
34	5509.0
35	6374.0
36	7428.5
37	8483.0
38	9727.5
39	10972.0
40	10972.0
41	12255.5
42	13539.0
43	15186.5
44	16834.0
45	18564.5
46	20295.0
47	20295.0
48	22080.5
49	23866.0
50	23644.0
51	23422.0
52	22608.0
53	21794.0
54	21794.0
55	20451.0
56	19108.0
57	18622.5
58	18137.0
59	17456.0
60	16775.0
61	16775.0
62	15474.5
63	14174.0
64	13025.0
65	11876.0
66	10538.0
67	9200.0
68	9200.0
69	7841.5
70	6483.0
71	5562.5
72	4642.0
73	3679.5
74	2717.0
75	2717.0
76	2203.5
77	1690.0
78	1416.0
79	1142.0
80	982.0
81	822.0
82	822.0
83	659.5
84	497.0
85	388.5
86	280.0
87	224.0
88	168.0
89	168.0
90	127.0
91	86.0
92	56.0
93	26.0
94	16.0
95	6.0
96	6.0
97	4.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	276961.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.12776892837583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.84155974036791	70.8353459767672
2	4.803264229484993	7.409301071873034
3	1.2583157060444394	2.9115324904422484
4	0.5904179776279816	1.8215048539859975
5	0.35723430132246337	1.377634232284437
6	0.2375975316398573	1.0995220510962826
7	0.15608519019663467	0.842695173783934
8	0.1132211056472292	0.6985993019339777
9	0.0919901571411829	0.6385496025303122
>10	0.4925949660410738	7.212510910925045
>50	0.04223348377040905	2.214290715580672
>100	0.014077827923469581	1.7510196930335453
>500	9.385218615646387E-4	0.6952684643070361
>1k	4.692609307823194E-4	0.4922254614563088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1360	0.49104386538176853	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	989	0.3570899873989479	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	932	0.3365094724528002	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	347	0.12528839800549535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	325	0.11734504135961382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0010831849971656659	0.0
13	0.0	0.0	0.0	0.0010831849971656659	3.610616657218886E-4
14	0.0	0.0	0.0	0.0014442466628875545	3.610616657218886E-4
15	0.0	0.0	0.0	0.0021663699943313317	3.610616657218886E-4
16	0.0	0.0	0.0	0.004332739988662663	3.610616657218886E-4
17	0.0	0.0	0.0	0.00794335664588155	3.610616657218886E-4
18	0.0	3.610616657218886E-4	0.0	0.009387603308769105	3.610616657218886E-4
19	0.0	3.610616657218886E-4	0.0	0.01047078830593477	3.610616657218886E-4
20	0.0	3.610616657218886E-4	0.0	0.011915034968822326	3.610616657218886E-4
21	0.0	3.610616657218886E-4	0.0	0.013720343297431768	3.610616657218886E-4
22	0.0	3.610616657218886E-4	0.0	0.02094157661186954	3.610616657218886E-4
23	0.0	0.0010831849971656659	0.0	0.0350229815750232	3.610616657218886E-4
24	0.0	0.0010831849971656659	0.0	0.05524243485544896	3.610616657218886E-4
25	0.0	0.0010831849971656659	0.0	0.06282472983560862	3.610616657218886E-4
26	0.0	0.0010831849971656659	0.0	0.08015568979025928	3.610616657218886E-4
27	0.0	0.0010831849971656659	0.0	0.18666888117821642	3.610616657218886E-4
28	0.0	0.0010831849971656659	0.0	0.5860030834666252	3.610616657218886E-4
29	0.0	0.0010831849971656659	0.0	1.173450413596138	3.610616657218886E-4
30	0.0	0.0010831849971656659	0.0	2.0374709796686177	3.610616657218886E-4
31	0.0	0.0010831849971656659	0.0	3.848917356595333	3.610616657218886E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACTA	30	3.5924886E-4	30.833334	5
ATACACA	245	0.0	24.91837	37
ATCGGCA	45	1.3196723E-4	24.666666	16
GCGTTAT	40	0.0019275078	23.125002	1
ATACCGT	40	0.0019275078	23.125002	6
CAATACA	45	0.003818589	20.555555	4
TAACCAG	55	5.129557E-4	20.181818	5
GGTATCA	835	0.0	19.27545	1
CGGGTAA	50	0.0070217364	18.499998	24
AGATACC	50	0.0070217364	18.499998	4
GGGTAAA	50	0.0070217364	18.499998	11
TAACCCG	50	0.0070217364	18.499998	28
GATAGTG	50	0.0070217364	18.499998	7
CAGTCGG	355	0.0	18.239437	10
CGGTGAT	350	0.0	17.971428	14
AGTCGGT	375	0.0	17.760002	11
GCAGTCG	365	0.0	17.739725	9
GTGATTC	360	0.0	17.472221	16
GGCAGTC	375	0.0	17.266668	8
TCGGTGA	365	0.0	17.232876	13
>>END_MODULE