FastQCFastQC Report
Fri 10 Feb 2017
ERR1631491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225347
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5530.2453993174970157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.19392314963145726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3920.17395394658016303No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10472737600234305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGC250.005487085329.5999983
CGCGGTA358.8471855E-426.42857231
GGTATCA2150.022.3720951
ATAACTG555.125474E-420.1818185
CAGATGT902.1366322E-618.54
TTCTACT500.00701776918.4999982
TATACAC1502.4738256E-1017.26666637
GCTCTGA650.001574609517.0769231
CCAGAGT700.002583828815.8571433
GTATCAA3200.015.6093752
TGCGGTG750.004090801314.85
TCCTAGG750.004090801314.82
CTTAGCT750.004090801314.84
CTTATAC3950.014.51898737
ACACCAT800.0062779213.8756
AGAAGTT800.0062779213.8755
GGACTAC800.0062779213.8756
GTGTGCT800.0062779213.8759
TGTGTGC800.0062779213.8758
AGCACTT850.00937407713.0588235
Produced by FastQC (version 0.11.5)