##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631491.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225347
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.05953041309625	34.0	33.0	34.0	31.0	34.0
2	33.20492839931306	34.0	33.0	34.0	31.0	34.0
3	33.28734351910609	34.0	34.0	34.0	31.0	34.0
4	36.5030242248621	37.0	37.0	37.0	35.0	37.0
5	36.4895294812001	37.0	37.0	37.0	35.0	37.0
6	36.543082446183	37.0	37.0	37.0	35.0	37.0
7	36.55174464270658	37.0	37.0	37.0	35.0	37.0
8	36.552720914855755	37.0	37.0	37.0	35.0	37.0
9	38.404021353734464	39.0	39.0	39.0	37.0	39.0
10	38.3701668981615	39.0	39.0	39.0	37.0	39.0
11	38.41786666785003	39.0	39.0	39.0	37.0	39.0
12	38.38717178395985	39.0	39.0	39.0	37.0	39.0
13	38.406772666154865	39.0	39.0	39.0	37.0	39.0
14	39.96480982662294	41.0	40.0	41.0	38.0	41.0
15	39.950964512507376	41.0	40.0	41.0	38.0	41.0
16	39.91940429648498	41.0	40.0	41.0	38.0	41.0
17	39.90922887813017	41.0	40.0	41.0	38.0	41.0
18	39.88588709856355	41.0	40.0	41.0	38.0	41.0
19	39.900726435231	41.0	40.0	41.0	38.0	41.0
20	39.864542239302054	41.0	40.0	41.0	38.0	41.0
21	39.8372820583367	41.0	40.0	41.0	38.0	41.0
22	39.779353619085235	41.0	40.0	41.0	38.0	41.0
23	39.73022937957905	41.0	40.0	41.0	38.0	41.0
24	39.696357173603374	41.0	40.0	41.0	37.0	41.0
25	39.70169560721909	41.0	40.0	41.0	37.0	41.0
26	39.59127922714747	41.0	40.0	41.0	37.0	41.0
27	39.54381465029488	41.0	40.0	41.0	37.0	41.0
28	39.50627698615912	41.0	40.0	41.0	37.0	41.0
29	39.453349722871835	41.0	39.0	41.0	36.0	41.0
30	39.38931958268803	41.0	39.0	41.0	36.0	41.0
31	39.33568674089293	41.0	39.0	41.0	36.0	41.0
32	39.26306096819572	41.0	39.0	41.0	35.0	41.0
33	39.19958552809667	41.0	39.0	41.0	35.0	41.0
34	39.139651293338716	41.0	39.0	41.0	35.0	41.0
35	39.07311834637248	41.0	39.0	41.0	35.0	41.0
36	39.00181054107665	41.0	39.0	41.0	35.0	41.0
37	38.940824594957995	41.0	39.0	41.0	35.0	41.0
38	38.87766422450709	41.0	39.0	41.0	35.0	41.0
39	38.8010889872064	41.0	39.0	41.0	35.0	41.0
40	38.71434720675225	40.0	38.0	41.0	35.0	41.0
41	38.62381127771836	40.0	38.0	41.0	35.0	41.0
42	38.51181511180535	40.0	38.0	41.0	35.0	41.0
43	37.74316054795493	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	1.0
18	3.0
19	6.0
20	12.0
21	23.0
22	55.0
23	87.0
24	111.0
25	200.0
26	279.0
27	436.0
28	593.0
29	860.0
30	1200.0
31	1470.0
32	1923.0
33	2575.0
34	3658.0
35	5742.0
36	9024.0
37	17143.0
38	41234.0
39	138708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.691728756096154	17.80276640026271	12.119974971932175	26.38552987170896
2	20.37879359387966	19.67410260620288	32.50453744669332	27.44256635322414
3	21.002276489147846	21.189543237762205	28.472089710535307	29.336090562554638
4	15.64609247072293	15.50364548895703	32.377622067300656	36.47263997301939
5	16.908146103564725	33.858449413571066	31.997763449258255	17.23564103360595
6	35.52521222825243	32.5981708210005	15.550240296076717	16.32637665467035
7	30.379814242035618	28.752989833456848	20.226583890622017	20.640612033885517
8	28.1934083879528	31.202989167816746	19.56316258925124	21.04043985497921
9	26.962417959857465	14.24869201720014	19.407846565518955	39.381043457423445
10	19.229011258192923	24.925559248625454	31.294403741784894	24.551025751396736
11	36.73046457241499	20.914411995722155	20.46710184737316	21.888021584489696
12	22.988102792582108	24.268794348271776	27.532649646988865	25.21045321215725
13	31.49720209277248	18.82430207635336	23.600935446222937	26.07756038465123
14	24.044695514029474	19.927933364988217	23.643536412732363	32.38383470824994
15	26.765832249819166	25.89783755718958	21.245900766373637	26.090429426617618
16	26.539958375305638	24.699685374111926	23.044904081261343	25.71545216932109
17	24.985466857779336	25.1931465695128	24.18359241525292	25.637794157454945
18	25.35112515365192	23.718088104123865	25.19758417019086	25.733202572033353
19	25.745184093864133	24.417897731054776	24.99301077893205	24.84390739614905
20	25.83748618796789	23.9581623008072	24.960172533914363	25.24417897731055
21	26.25018305102797	23.949730859518876	24.56700111383777	25.233084975615384
22	26.57723422100139	23.803733797210526	24.28255091037378	25.33648107141431
23	25.881862194748546	23.90934869334848	24.39881604813909	25.809973063763884
24	25.6142748738612	24.23506858311848	24.286988511051845	25.863668031968473
25	25.959520206614688	23.845891003652145	24.444523335123165	25.75006545461
26	25.69282040586296	24.208442979050087	24.645546645839527	25.453189969247425
27	25.597855751352363	23.875179168127378	24.595845518245195	25.931119562275068
28	25.116376077782267	23.834353241889175	25.139451601308206	25.90981907902036
29	25.003661020559402	24.038926633147987	25.45984637026453	25.49756597602808
30	24.891833483472155	24.41701021091916	25.135457760697943	25.555698544910737
31	25.558804865385383	24.09927800236968	24.876745641166735	25.465171491078202
32	25.06179358944206	23.933311737010033	25.094631834459747	25.910262839088162
33	24.73252361912961	23.807727637820783	25.442095967552262	26.017652775497346
34	25.221547213852414	23.042685280922313	25.453633729315232	26.282133775910037
35	24.89360852374338	23.031591279227147	26.162318557602276	25.91248163942719
36	24.316276675527078	23.124337133398715	26.16586863814473	26.39351755292948
37	24.45650485695394	22.18267826951324	26.82352105863402	26.537295814898798
38	23.594279045205838	21.86849614150621	28.17033286442686	26.36689194886109
39	23.50020191083085	21.77486276719903	28.416175941991682	26.308759379978436
40	23.043572801057923	21.893346705303376	29.013920753327092	26.049159740311605
41	22.29894340727855	21.934616391609385	29.2655327117734	26.500907489338665
42	21.971448477237328	22.323350211007913	29.374253928386	26.33094738336876
43	21.43671759553045	22.074844573036252	29.522913551101194	26.965524280332108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	61.0
2	63.0
3	111.5
4	160.0
5	160.0
6	192.5
7	225.0
8	204.5
9	184.0
10	273.0
11	362.0
12	362.0
13	583.5
14	805.0
15	977.0
16	1149.0
17	1143.5
18	1138.0
19	1138.0
20	1392.5
21	1647.0
22	1577.5
23	1508.0
24	1764.5
25	2021.0
26	2021.0
27	2284.5
28	2548.0
29	3052.0
30	3556.0
31	4161.0
32	4766.0
33	4766.0
34	5575.0
35	6384.0
36	7222.0
37	8060.0
38	8822.5
39	9585.0
40	9585.0
41	10459.5
42	11334.0
43	11925.5
44	12517.0
45	13300.5
46	14084.0
47	14084.0
48	14628.5
49	15173.0
50	15767.0
51	16361.0
52	16323.0
53	16285.0
54	16285.0
55	16178.0
56	16071.0
57	15746.5
58	15422.0
59	14908.0
60	14394.0
61	14394.0
62	13459.0
63	12524.0
64	11578.5
65	10633.0
66	9377.5
67	8122.0
68	8122.0
69	7132.0
70	6142.0
71	5266.5
72	4391.0
73	3588.0
74	2785.0
75	2785.0
76	2279.5
77	1774.0
78	1495.0
79	1216.0
80	1028.0
81	840.0
82	840.0
83	660.0
84	480.0
85	386.5
86	293.0
87	238.5
88	184.0
89	184.0
90	130.5
91	77.0
92	46.5
93	16.0
94	11.5
95	7.0
96	7.0
97	3.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	225347.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.6307836302479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.78874308625345	77.59995296369124
2	4.587446432957291	7.673034801000076
3	1.0324159890570082	2.5902527459170512
4	0.49728288383348956	1.6635262904361707
5	0.27691001224233114	1.1579100659443846
6	0.174156566515868	0.873891007924526
7	0.1295446559286222	0.758374476329506
8	0.08801519162296095	0.5888623557435724
9	0.07455076784490429	0.5611265221595431
>10	0.3333909270915543	4.905393122789013
>50	0.01435376180663722	0.7767138369760912
>100	0.002658104038266152	0.6050987590866972
>500	5.316208076532304E-4	0.2458630520021628
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	553	0.2453993174970157	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	437	0.19392314963145726	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.17395394658016303	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.10472737600234305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.4376006780653835E-4	0.0
5	0.0	0.0	0.0	4.4376006780653835E-4	0.0
6	0.0	0.0	0.0	4.4376006780653835E-4	0.0
7	0.0	0.0	0.0	4.4376006780653835E-4	0.0
8	0.0	0.0	0.0	4.4376006780653835E-4	0.0
9	0.0	0.0	0.0	0.0013312802034196151	0.0
10	0.0	0.0	0.0	0.0017750402712261534	0.0
11	0.0	0.0	0.0	0.0017750402712261534	0.0
12	0.0	0.0	0.0	0.0022188003390326916	0.0
13	0.0	0.0	0.0	0.0026625604068392303	0.0
14	0.0	0.0	0.0	0.0035500805424523068	0.0
15	0.0	0.0	0.0	0.004881360745871922	0.0
16	0.0	0.0	0.0	0.005768880881484998	0.0
17	0.0	0.0	0.0	0.006656401017098076	0.0
18	0.0	0.0	0.0	0.00798768122051769	0.0
19	0.0	0.0	0.0	0.009318961423937306	0.0
20	0.0	0.0	0.0	0.009762721491743844	4.4376006780653835E-4
21	0.0	0.0	0.0	0.011537761762969997	4.4376006780653835E-4
22	0.0	4.4376006780653835E-4	0.0	0.017750402712261533	4.4376006780653835E-4
23	0.0	4.4376006780653835E-4	0.0	0.025294323864972688	4.4376006780653835E-4
24	0.0	4.4376006780653835E-4	0.0	0.040382166170394994	4.4376006780653835E-4
25	0.0	4.4376006780653835E-4	0.0	0.050144887662138836	4.4376006780653835E-4
26	0.0	4.4376006780653835E-4	0.0	0.06345768969633499	4.4376006780653835E-4
27	0.0	4.4376006780653835E-4	0.0	0.16374746502061266	4.4376006780653835E-4
28	0.0	4.4376006780653835E-4	0.0	0.39006509960194724	4.4376006780653835E-4
29	0.0	8.875201356130767E-4	0.0	0.652771059743418	4.4376006780653835E-4
30	0.0	8.875201356130767E-4	0.0	1.0601428019898202	4.4376006780653835E-4
31	0.0	8.875201356130767E-4	0.0	1.6827381771223935	4.4376006780653835E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAAGC	25	0.0054870853	29.599998	3
CGCGGTA	35	8.8471855E-4	26.428572	31
GGTATCA	215	0.0	22.372095	1
ATAACTG	55	5.125474E-4	20.181818	5
CAGATGT	90	2.1366322E-6	18.5	4
TTCTACT	50	0.007017769	18.499998	2
TATACAC	150	2.4738256E-10	17.266666	37
GCTCTGA	65	0.0015746095	17.076923	1
CCAGAGT	70	0.0025838288	15.857143	3
GTATCAA	320	0.0	15.609375	2
TGCGGTG	75	0.0040908013	14.8	5
TCCTAGG	75	0.0040908013	14.8	2
CTTAGCT	75	0.0040908013	14.8	4
CTTATAC	395	0.0	14.518987	37
ACACCAT	80	0.00627792	13.875	6
AGAAGTT	80	0.00627792	13.875	5
GGACTAC	80	0.00627792	13.875	6
GTGTGCT	80	0.00627792	13.875	9
TGTGTGC	80	0.00627792	13.875	8
AGCACTT	85	0.009374077	13.058823	5
>>END_MODULE