FastQCFastQC Report
Fri 10 Feb 2017
ERR1631490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75622
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16792.2202533654227605No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14541.922720901325011No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11421.5101425511094655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.7973870037819683No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2480.32794689376107483No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.32530216074687257No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.2314141387426939No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.22744703922139062No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT1670.22083520668588508No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT1670.22083520668588508No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA1550.20496680860067176No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1520.20099970907936845No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC1470.1943878765438629No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.19174314352966068No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1320.17455237893734626No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1230.16265108037343629No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.16000634735923408No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.15603924783793077No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG1170.15471688133082967No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1130.14942741530242523No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC1100.1454603157811219No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.1454603157811219No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1060.14017084975271746No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1060.14017084975271746No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC1060.14017084975271746No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC1040.13752611673851525No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC1030.13620375023141415No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1000.13223665071011081No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG1000.13223665071011081No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT980.1295919176959086No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA950.12562481817460527No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG940.12430245166750416No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT920.12165771865330194No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA900.11901298563909972No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA900.11901298563909972No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG880.11636825262489753No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG840.11107878659649309No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA840.11107878659649309No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.10975642008939199No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT820.10843405358229087No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG810.10711168707518977No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.10711168707518977No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.10578932056808865No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG790.10446695406098755No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC790.10446695406098755No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC770.10182222104678533No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC770.10182222104678533No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC760.10049985453968423No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG760.10049985453968423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCT405.8681562E-527.7533
ACCGACC451.3073086E-424.66666632
GGCGTCC400.001914637923.12515
GTCCCCC400.001914637923.12518
GCGTCCC400.001914637923.12516
CGTCCCC400.001914637923.12517
CGACCTG502.6698882E-422.19999934
TTGCCAA502.6698882E-422.19999928
CGGCGTC450.003793335320.55555514
TCCCCCA450.003793335320.55555519
GCCTTAT1450.020.41379426
TCCTCCG555.082276E-420.18181821
TTCCTCC555.082276E-420.18181820
TGCCAAG555.082276E-420.18181829
TTTGCCA555.082276E-420.18181827
GGTGATT2050.019.8536615
GTGATTC2050.019.8536616
GGTATCA7400.019.7500021
TTATACA1600.019.6562537
CAGTCGG2100.019.3809510
Produced by FastQC (version 0.11.5)