##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631490.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75622
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70717516066753	34.0	31.0	34.0	31.0	34.0
2	32.82144085054614	34.0	31.0	34.0	31.0	34.0
3	32.87902991193039	34.0	33.0	34.0	31.0	34.0
4	36.27388855095078	37.0	37.0	37.0	35.0	37.0
5	36.26883711089366	37.0	37.0	37.0	35.0	37.0
6	36.3455872629658	37.0	37.0	37.0	35.0	37.0
7	36.353402449022774	37.0	37.0	37.0	35.0	37.0
8	36.38035227843749	37.0	37.0	37.0	35.0	37.0
9	38.178426912803154	39.0	39.0	39.0	37.0	39.0
10	38.16442305149295	39.0	39.0	39.0	37.0	39.0
11	38.223850202322076	39.0	39.0	39.0	37.0	39.0
12	38.03871889132792	39.0	39.0	39.0	37.0	39.0
13	38.164356933167596	39.0	39.0	39.0	37.0	39.0
14	39.55187643807358	41.0	39.0	41.0	37.0	41.0
15	39.510129327444396	41.0	39.0	41.0	37.0	41.0
16	39.4199439316601	41.0	39.0	41.0	37.0	41.0
17	39.498003226574276	41.0	39.0	41.0	37.0	41.0
18	39.57212186929729	41.0	39.0	41.0	37.0	41.0
19	39.58159001348814	41.0	40.0	41.0	37.0	41.0
20	39.57031022718257	41.0	40.0	41.0	37.0	41.0
21	39.55282854195869	41.0	40.0	41.0	37.0	41.0
22	39.42120017984185	41.0	39.0	41.0	36.0	41.0
23	39.30614107005898	41.0	39.0	41.0	35.0	41.0
24	39.22180053423607	41.0	39.0	41.0	35.0	41.0
25	39.237404459019864	41.0	39.0	41.0	35.0	41.0
26	39.061066885297926	41.0	39.0	41.0	35.0	41.0
27	38.907196318531646	40.0	38.0	41.0	35.0	41.0
28	38.70852397450477	40.0	38.0	41.0	35.0	41.0
29	38.54041152045701	40.0	38.0	41.0	35.0	41.0
30	38.34005977096612	40.0	37.0	41.0	35.0	41.0
31	38.122781730184336	40.0	36.0	41.0	35.0	41.0
32	37.913490783105445	40.0	35.0	41.0	35.0	41.0
33	37.6565549707757	40.0	35.0	41.0	34.0	41.0
34	37.51689984396075	40.0	35.0	41.0	34.0	41.0
35	37.31211816667108	40.0	35.0	41.0	33.0	41.0
36	37.13551611964772	40.0	35.0	41.0	33.0	41.0
37	36.918105842215226	40.0	35.0	41.0	33.0	41.0
38	36.661950226124674	39.0	35.0	41.0	33.0	41.0
39	36.35752823252493	39.0	35.0	41.0	31.0	41.0
40	36.03497659411283	39.0	35.0	41.0	30.0	41.0
41	35.69895004099336	39.0	35.0	41.0	28.0	41.0
42	35.318915130517574	39.0	35.0	41.0	24.0	41.0
43	34.32736505249795	37.0	35.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	0.0
15	2.0
16	1.0
17	0.0
18	4.0
19	3.0
20	7.0
21	13.0
22	25.0
23	41.0
24	84.0
25	161.0
26	230.0
27	405.0
28	557.0
29	741.0
30	1014.0
31	1185.0
32	1432.0
33	1796.0
34	2453.0
35	3317.0
36	5309.0
37	11824.0
38	14989.0
39	30025.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.03789902409351	17.33886964110973	14.66504456375129	20.95818677104546
2	19.633175530930153	22.75263812118167	32.547406839279574	25.066779508608604
3	21.37473222078231	24.841977202401416	29.31686546243157	24.466425114384702
4	13.702361746581682	17.44994842770622	31.620427917801695	37.227261907910396
5	13.827986564756287	39.083864483880355	29.8352331332152	17.252915818148157
6	29.699029382983788	33.21652429187274	18.52106529845812	18.563381026685356
7	27.8027558118008	32.96924175504483	19.253656343392134	19.97434608976224
8	33.26809658564968	28.239136759144163	18.75512417021502	19.73764248499114
9	29.19653011028537	12.731744730369469	20.15418793472799	37.91753722461718
10	21.951284017878393	26.857263759223503	25.048266377509194	26.143185845388906
11	34.1342466478009	24.77189177752506	17.1867974927931	23.907064081880936
12	22.785697283859193	30.96717886329375	24.984792785168334	21.26233106767872
13	32.17317711776996	18.94686731374468	26.44204067599376	22.4379148924916
14	23.955991642643674	22.35989526857264	26.353442120017984	27.330670968765702
15	28.65832694189522	25.812594218613633	23.19827563407474	22.330803205416412
16	23.420433207267727	25.926317738224324	26.948507048213482	23.704742006294467
17	19.947898759620216	30.052101240379788	26.14450821189601	23.85549178810399
18	22.083520668588505	22.695776361376318	30.750310756129167	24.470392213906006
19	21.35092962365449	28.129380339054773	30.860067176218557	19.659622861072172
20	23.987728438814102	22.841236677157443	31.699769908227765	21.471264975800693
21	26.89561238792944	23.55663695749914	29.91986458966967	19.627886064901748
22	25.923673005210123	23.335801750813257	28.610721747639577	22.129803496337043
23	22.13641532887255	25.01256248181746	29.073550025124963	23.777472164185028
24	20.375023141413877	26.571632593689664	31.457776837428263	21.595567427468197
25	20.901325011240115	25.595726111449054	29.94366718679749	23.55928169051334
26	22.63494750204967	26.38385654968131	29.70564121551929	21.27555473274973
27	23.421755573774828	24.48493824548412	29.359181190658802	22.734124990082254
28	19.97566845626934	25.474068392795747	32.14672978762793	22.403533363306973
29	19.5723466716035	26.40898151331623	32.77882097802227	21.239850837058
30	19.23117611277142	27.880775435719762	33.39636613683849	19.491682314670335
31	19.84739890508053	28.10557774192695	29.9516013858401	22.095421967152415
32	20.269233820845788	26.77924413530454	31.712993573298775	21.2385284705509
33	19.175636719473168	26.31112639179075	32.367564994313824	22.145671894422257
34	21.36944275475391	26.855941392716403	29.950279019333	21.82433683319669
35	20.568088651450637	26.551797096083153	30.680225331252807	22.199888921213404
36	16.84694930046812	28.00904498690857	31.231652164713974	23.912353547909337
37	19.701938589299413	26.358731586046392	32.05548649863796	21.88384332601624
38	18.55544682764275	23.899129882838327	33.44793843061543	24.097484858903492
39	20.1634445002777	24.71370765121261	33.31966887942662	21.80317896908307
40	18.65991378170374	24.65420115839306	33.044616645949596	23.64126841395361
41	16.01385840099442	25.821850784163335	34.97791647933141	23.18637433551083
42	17.28068551479728	25.218851656925235	33.819523419110844	23.680939409166648
43	15.692523339768849	25.503160455951974	34.275739864060725	24.528576340218457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	86.0
2	60.0
3	132.5
4	205.0
5	205.0
6	278.0
7	351.0
8	350.5
9	350.0
10	568.5
11	787.0
12	787.0
13	1383.0
14	1979.0
15	2742.0
16	3505.0
17	3036.0
18	2567.0
19	2567.0
20	2772.5
21	2978.0
22	2025.0
23	1072.0
24	933.0
25	794.0
26	794.0
27	741.0
28	688.0
29	695.0
30	702.0
31	771.5
32	841.0
33	841.0
34	932.5
35	1024.0
36	1200.0
37	1376.0
38	1684.5
39	1993.0
40	1993.0
41	2494.0
42	2995.0
43	3333.5
44	3672.0
45	4400.5
46	5129.0
47	5129.0
48	6200.0
49	7271.0
50	6643.0
51	6015.0
52	5510.5
53	5006.0
54	5006.0
55	4657.0
56	4308.0
57	4089.5
58	3871.0
59	3872.0
60	3873.0
61	3873.0
62	3698.0
63	3523.0
64	3179.5
65	2836.0
66	2536.0
67	2236.0
68	2236.0
69	1799.5
70	1363.0
71	1142.5
72	922.0
73	679.5
74	437.0
75	437.0
76	355.0
77	273.0
78	244.0
79	215.0
80	175.0
81	135.0
82	135.0
83	101.0
84	67.0
85	53.0
86	39.0
87	33.5
88	28.0
89	28.0
90	22.0
91	16.0
92	10.0
93	4.0
94	3.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	75622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.51204675887969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63869451418194	41.4112295363783
2	7.125687730356284	7.056147681891513
3	2.734896640136745	4.062309909814604
4	1.4529138400726458	2.8774695194520117
5	1.0202446450510123	2.5257200285631165
6	0.7211153250360558	2.1422337415037953
7	0.47006036002350304	1.6291555367485653
8	0.3792532450189627	1.5022083520668588
9	0.2777629400138881	1.2377350506466374
>10	1.9283157950964156	18.740578138636906
>50	0.17894343250894718	6.0762741001295915
>100	0.061428342503071415	4.288434582528894
>500	0.00267079750013354	0.7973870037819683
>1k	0.008012392500400619	5.653116817857237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	2.2202533654227605	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1454	1.922720901325011	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1142	1.5101425511094655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.7973870037819683	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	248	0.32794689376107483	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	246	0.32530216074687257	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	175	0.2314141387426939	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	172	0.22744703922139062	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	167	0.22083520668588508	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	167	0.22083520668588508	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	155	0.20496680860067176	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	152	0.20099970907936845	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	147	0.1943878765438629	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	145	0.19174314352966068	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	132	0.17455237893734626	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	123	0.16265108037343629	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.16000634735923408	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	118	0.15603924783793077	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	117	0.15471688133082967	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	113	0.14942741530242523	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	110	0.1454603157811219	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	110	0.1454603157811219	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	106	0.14017084975271746	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	106	0.14017084975271746	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	106	0.14017084975271746	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	104	0.13752611673851525	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	103	0.13620375023141415	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	100	0.13223665071011081	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG	100	0.13223665071011081	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	98	0.1295919176959086	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	95	0.12562481817460527	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	94	0.12430245166750416	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	92	0.12165771865330194	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	90	0.11901298563909972	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	90	0.11901298563909972	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	88	0.11636825262489753	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	84	0.11107878659649309	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	84	0.11107878659649309	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	83	0.10975642008939199	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	82	0.10843405358229087	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG	81	0.10711168707518977	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	81	0.10711168707518977	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	80	0.10578932056808865	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	79	0.10446695406098755	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	79	0.10446695406098755	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	77	0.10182222104678533	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	77	0.10182222104678533	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	76	0.10049985453968423	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	76	0.10049985453968423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0013223665071011082	0.0
10	0.0	0.0	0.0	0.0013223665071011082	0.0
11	0.0	0.0	0.0	0.003967099521303324	0.0
12	0.0	0.0	0.0	0.005289466028404433	0.0
13	0.0	0.0	0.0	0.005289466028404433	0.0
14	0.0	0.0	0.0	0.005289466028404433	0.0
15	0.0	0.0	0.0	0.010578932056808866	0.0
16	0.0	0.0	0.0	0.017190764592314407	0.0
17	0.0	0.0	0.0	0.025124963634921053	0.0
18	0.0	0.0	0.0	0.02909206315622438	0.0
19	0.0	0.0	0.0	0.037026262198831025	0.0
20	0.0	0.0	0.0	0.046282827748538785	0.0
21	0.0	0.0	0.0	0.062151225833752086	0.0
22	0.0	0.0	0.0	0.07273015789056095	0.0
23	0.0	0.0	0.0	0.09653275501838089	0.0
24	0.0	0.0	0.0	0.13223665071011081	0.0
25	0.0	0.0	0.0	0.15339451482372854	0.0
26	0.0	0.0	0.0	0.1798418449657507	0.0
27	0.0	0.0	0.0	0.2631509349131205	0.0
28	0.0	0.0	0.0	0.5289466028404433	0.0
29	0.0	0.0	0.0	0.9005315913358547	0.0
30	0.0	0.0	0.0	1.492951786517151	0.0
31	0.0	0.0	0.0	2.55348972521224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACCT	40	5.8681562E-5	27.75	33
ACCGACC	45	1.3073086E-4	24.666666	32
GGCGTCC	40	0.0019146379	23.125	15
GTCCCCC	40	0.0019146379	23.125	18
GCGTCCC	40	0.0019146379	23.125	16
CGTCCCC	40	0.0019146379	23.125	17
CGACCTG	50	2.6698882E-4	22.199999	34
TTGCCAA	50	2.6698882E-4	22.199999	28
CGGCGTC	45	0.0037933353	20.555555	14
TCCCCCA	45	0.0037933353	20.555555	19
GCCTTAT	145	0.0	20.413794	26
TCCTCCG	55	5.082276E-4	20.181818	21
TTCCTCC	55	5.082276E-4	20.181818	20
TGCCAAG	55	5.082276E-4	20.181818	29
TTTGCCA	55	5.082276E-4	20.181818	27
GGTGATT	205	0.0	19.85366	15
GTGATTC	205	0.0	19.85366	16
GGTATCA	740	0.0	19.750002	1
TTATACA	160	0.0	19.65625	37
CAGTCGG	210	0.0	19.38095	10
>>END_MODULE