FastQCFastQC Report
Fri 10 Feb 2017
ERR1631489.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631489.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73088
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.47750656742556913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3450.4720337127845885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2340.32016199649737304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.1628174255691769No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCC451.3067167E-424.66666817
CTGAGCC502.6686894E-422.214
GGGTAGG502.6686894E-422.21
ACACGCT450.003792124820.5555579
CACGCTG450.003792124820.55555710
GCTGAGC450.003792124820.55555713
ACGCTGA450.003792124820.55555711
CACACGC450.003792124820.5555578
TACCTGT759.072604E-619.73333437
GCACACG500.00697353818.57
CTTATAC902.0966672E-618.537
TCTATGG500.00697353818.52
TCTTATA1451.4551915E-1017.86206837
GGTATCA1800.017.4722231
GGTAGGC650.001560870317.0769232
CCAGTCA855.279273E-415.23529419
GAAATTG750.004055693714.813
AAATTGA750.004055693714.814
AGCTGTC950.001225654913.63157936
CTCTTAT2001.3789759E-812.95000136
Produced by FastQC (version 0.11.5)