##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	73088
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.657317206654994	34.0	31.0	34.0	31.0	34.0
2	32.83387149737303	34.0	31.0	34.0	31.0	34.0
3	32.93678852889667	34.0	33.0	34.0	31.0	34.0
4	36.31125492556918	37.0	37.0	37.0	35.0	37.0
5	36.28216670315236	37.0	37.0	37.0	35.0	37.0
6	36.346554838003506	37.0	37.0	37.0	35.0	37.0
7	36.350864711033275	37.0	37.0	37.0	35.0	37.0
8	36.349250218914186	37.0	37.0	37.0	35.0	37.0
9	38.18238288091068	39.0	39.0	39.0	37.0	39.0
10	38.11747482486865	39.0	38.0	39.0	37.0	39.0
11	38.190304838003506	39.0	39.0	39.0	37.0	39.0
12	37.96974879597198	39.0	38.0	39.0	37.0	39.0
13	38.11011383537653	39.0	38.0	39.0	37.0	39.0
14	39.552197351138354	41.0	39.0	41.0	37.0	41.0
15	39.54008866024518	41.0	39.0	41.0	37.0	41.0
16	39.49213277145359	41.0	39.0	41.0	37.0	41.0
17	39.5133811295972	41.0	39.0	41.0	37.0	41.0
18	39.50622537215411	41.0	39.0	41.0	37.0	41.0
19	39.525982377408056	41.0	39.0	41.0	37.0	41.0
20	39.47412707968476	41.0	39.0	41.0	37.0	41.0
21	39.45309763572679	41.0	39.0	41.0	37.0	41.0
22	39.37467162872154	41.0	39.0	41.0	36.0	41.0
23	39.31969680385289	41.0	39.0	41.0	36.0	41.0
24	39.286011383537655	41.0	39.0	41.0	36.0	41.0
25	39.26800569176883	41.0	39.0	41.0	36.0	41.0
26	39.156551007005255	41.0	39.0	41.0	36.0	41.0
27	39.08905702714536	40.0	39.0	41.0	35.0	41.0
28	39.04318082311734	40.0	39.0	41.0	35.0	41.0
29	38.97148642732049	40.0	39.0	41.0	35.0	41.0
30	38.881553743432576	40.0	38.0	41.0	35.0	41.0
31	38.791716834500875	40.0	38.0	41.0	35.0	41.0
32	38.712456217162874	40.0	38.0	41.0	35.0	41.0
33	38.61684544658494	40.0	38.0	41.0	35.0	41.0
34	38.579725809982484	40.0	38.0	41.0	35.0	41.0
35	38.45695599824869	40.0	38.0	41.0	35.0	41.0
36	38.39259522767075	40.0	38.0	41.0	35.0	41.0
37	38.29449430823117	40.0	38.0	41.0	34.0	41.0
38	38.18689798598949	40.0	38.0	41.0	34.0	41.0
39	38.09005582311734	40.0	37.0	41.0	34.0	41.0
40	37.9927621497373	40.0	37.0	41.0	34.0	41.0
41	37.88044549036778	40.0	37.0	41.0	34.0	41.0
42	37.7405182793345	40.0	37.0	41.0	33.0	41.0
43	36.85150777145359	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	0.0
17	2.0
18	1.0
19	6.0
20	13.0
21	14.0
22	26.0
23	39.0
24	66.0
25	98.0
26	155.0
27	210.0
28	282.0
29	428.0
30	573.0
31	784.0
32	926.0
33	1250.0
34	1795.0
35	2570.0
36	3888.0
37	7828.0
38	17364.0
39	34768.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.47072022767075	20.05390761821366	12.817425569176882	24.657946584938703
2	20.01970227670753	20.31523642732049	33.83181917688266	25.833242119089316
3	19.899299474605954	23.34719789842382	29.479531523642734	27.273971103327494
4	14.645359019264447	17.08214754816112	34.74031304728547	33.53218038528897
5	14.665882224168126	36.56687828371278	33.57596322241681	15.191276269702279
6	31.85201401050788	35.636492994746064	16.262587565674256	16.248905429071804
7	28.76669220665499	30.21836690017513	21.907837127845884	19.107103765323995
8	27.566768826619963	31.878010070052536	19.66259851138354	20.892622591943958
9	26.56523642732049	14.390871278458844	20.891254378283712	38.15263791593696
10	19.888353765323995	25.647165061295972	30.887423380035028	23.57705779334501
11	35.065947898423815	22.505746497373032	20.858417250437828	21.569888353765325
12	22.080232049036777	27.711799474605954	26.47356611208406	23.734402364273205
13	30.45506786339755	19.584610332749563	24.72909369527145	25.231228108581437
14	23.345829684763572	21.304454903677758	24.718147985989493	30.631567425569177
15	26.970227670753065	26.98801444833625	21.706709719789842	24.335048161120838
16	25.11903458844133	25.305111646234675	24.68667907180385	24.88917469352014
17	23.34309325744308	26.899080560420312	24.638791593695274	25.11903458844133
18	23.300678633975483	24.72088441330998	26.505035026269702	25.473401926444833
19	24.484183450087567	25.651269702276707	26.840247373029776	23.024299474605954
20	24.24885070052539	24.927484676007005	26.829301663747813	23.99436295971979
21	25.545917250437828	24.768771891418563	26.030264886164623	23.655045971978982
22	26.028896672504377	24.425350262697023	25.651269702276707	23.894483362521893
23	24.49923380035026	24.65657837127846	26.7321584938704	24.112029334500875
24	23.364984676007005	26.10688485113835	25.285956654991242	25.2421738178634
25	24.466396672504377	24.618268388791595	26.327167250437828	24.5881676882662
26	23.78092162872154	25.70463003502627	26.379159369527144	24.135288966725042
27	23.827440893169875	25.54318082311734	25.797668563922944	24.831709719789842
28	23.37729859894921	25.273642732049034	27.69948555166375	23.649573117338004
29	23.737138791593697	25.079356392294223	27.321858581436075	23.861646234676005
30	23.61126313485114	25.332475919439577	27.02495621716287	24.03130472854641
31	24.112029334500875	25.4118323117338	26.680166374781084	23.795971978984237
32	23.204903677758317	25.19018169877408	26.76910026269702	24.835814360770577
33	22.336088003502628	25.127243870402804	27.33143607705779	25.20523204903678
34	24.225591068301224	23.91227014010508	27.107049036777582	24.75508975481611
35	23.71251094570928	24.549857705779335	27.490148861646237	24.24748248686515
36	23.132388353765325	24.47186952714536	26.796464535901926	25.59927758318739
37	23.296573992994745	23.13649299474606	27.78705122591944	25.779881786339754
38	22.602889667250437	23.029772329246935	29.110113835376534	25.257224168126097
39	22.21021234676007	23.293837565674256	29.3180823117338	25.177867775831874
40	21.84079465849387	23.11870621716287	30.479695709281962	24.560803415061297
41	21.11427320490368	22.94904772329247	30.47695928196147	25.45971978984238
42	20.929564360770577	23.704301663747813	30.45643607705779	24.90969789842382
43	20.281031085814362	22.816330998248688	30.85732267950963	26.045315236427317
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	16.5
2	16.0
3	37.0
4	58.0
5	58.0
6	72.0
7	86.0
8	84.5
9	83.0
10	125.5
11	168.0
12	168.0
13	270.0
14	372.0
15	538.5
16	705.0
17	610.5
18	516.0
19	516.0
20	578.0
21	640.0
22	467.0
23	294.0
24	308.5
25	323.0
26	323.0
27	416.5
28	510.0
29	748.5
30	987.0
31	1353.0
32	1719.0
33	1719.0
34	2138.0
35	2557.0
36	2988.5
37	3420.0
38	3780.0
39	4140.0
40	4140.0
41	4709.5
42	5279.0
43	5554.5
44	5830.0
45	6006.5
46	6183.0
47	6183.0
48	6204.0
49	6225.0
50	6093.5
51	5962.0
52	5562.0
53	5162.0
54	5162.0
55	4824.5
56	4487.0
57	3988.0
58	3489.0
59	3319.5
60	3150.0
61	3150.0
62	2907.0
63	2664.0
64	2434.0
65	2204.0
66	1954.5
67	1705.0
68	1705.0
69	1454.5
70	1204.0
71	1081.5
72	959.0
73	762.5
74	566.0
75	566.0
76	477.0
77	388.0
78	329.5
79	271.0
80	253.0
81	235.0
82	235.0
83	215.0
84	195.0
85	160.0
86	125.0
87	110.5
88	96.0
89	96.0
90	81.0
91	66.0
92	43.0
93	20.0
94	14.0
95	8.0
96	8.0
97	5.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	73088.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.29580779334501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83460028591527	48.739875218914186
2	8.509383013002338	10.261602451838879
3	3.732782681703692	6.752134413309982
4	1.9378701581610658	4.673817863397548
5	1.2253511538723365	3.6941768826619965
6	0.8169007692482244	2.955341506129597
7	0.5967913953118973	2.5188813485113837
8	0.4492954230865234	2.167250437828371
9	0.3267603076992897	1.7732049036777584
>10	1.5543806303750936	14.798598949211907
>50	0.00680750641040187	0.23259632224168128
>100	0.00907667521386916	1.4325197022767076
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.47750656742556913	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.4720337127845885	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	234	0.32016199649737304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119	0.1628174255691769	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.002736427320490368	0.0
24	0.0	0.0	0.0	0.008209281961471103	0.0
25	0.0	0.0	0.0	0.019154991243432576	0.0
26	0.0	0.0	0.0	0.02736427320490368	0.0
27	0.0	0.0	0.0	0.08893388791593695	0.0
28	0.0	0.0	0.0	0.2640652364273205	0.0
29	0.0	0.0	0.0	0.4651926444833625	0.0
30	0.0	0.0	0.0	0.7210485989492119	0.0
31	0.0	0.0	0.0	1.3230626094570928	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATTCC	45	1.3067167E-4	24.666668	17
CTGAGCC	50	2.6686894E-4	22.2	14
GGGTAGG	50	2.6686894E-4	22.2	1
ACACGCT	45	0.0037921248	20.555557	9
CACGCTG	45	0.0037921248	20.555557	10
GCTGAGC	45	0.0037921248	20.555557	13
ACGCTGA	45	0.0037921248	20.555557	11
CACACGC	45	0.0037921248	20.555557	8
TACCTGT	75	9.072604E-6	19.733334	37
GCACACG	50	0.006973538	18.5	7
CTTATAC	90	2.0966672E-6	18.5	37
TCTATGG	50	0.006973538	18.5	2
TCTTATA	145	1.4551915E-10	17.862068	37
GGTATCA	180	0.0	17.472223	1
GGTAGGC	65	0.0015608703	17.076923	2
CCAGTCA	85	5.279273E-4	15.235294	19
GAAATTG	75	0.0040556937	14.8	13
AAATTGA	75	0.0040556937	14.8	14
AGCTGTC	95	0.0012256549	13.631579	36
CTCTTAT	200	1.3789759E-8	12.950001	36
>>END_MODULE