FastQCFastQC Report
Fri 10 Feb 2017
ERR1631488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246676
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58662.378018129043766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50532.04843600512413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40491.6414243785370284No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA18660.7564578637565065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.6871361624154761No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA11120.45079375374985814No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9450.383093612674115No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT9450.383093612674115No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT9190.37255347094974783No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT9180.37214808088342605No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT8840.3583648186284843No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA8370.33931148551135903No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC8300.3364737550471063No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC8170.33120368418492274No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA8010.3247174431237737No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA6890.2793137556957304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.2659358835071105No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.2635035431091796No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA6400.25944964244596147No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.23350467820136533No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT5710.2314777278697563No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA5360.21728907554849275No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA5200.21080283448734372No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT5060.20512737355883834No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT4920.1994519126303329No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT4840.19620879209975836No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT4790.19418184176814932No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG4440.1799931894468858No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC4120.1670207073245877No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG4040.1637775867940132No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3970.1609398563297605No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.15404822520228964No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3700.1499943245390715No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA3620.14675120400849698No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA3420.13864340268206066No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC3400.13783262254941706No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA3390.1374272324830952No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC3300.1337787218861989No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT3060.12404936029447534No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT3040.12323858016183172No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.1228331900955099No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.12202240996286627No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT2980.12080623976390083No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC2970.12040084969757901No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT2900.11756311923332631No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT2830.1147253887690736No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT2810.11391460863642998No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA2780.11269843843746453ABI Solid3 Adapter B (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.1118876583048209No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT2730.11067148810585546No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT2640.10702297750895913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT200.001839134837.01
TATTGCT401.593331E-632.37530
CAGAAAT303.5913583E-430.8333342
TATGGCA303.5913583E-430.8333344
AGAAATC303.5913583E-430.8333343
TTATTGC453.9914994E-628.77777729
ATATGCT706.539267E-926.42857235
TATGCTG706.539267E-926.42857236
TAACGAT358.849576E-426.42857223
GTCTTCT358.849576E-426.42857219
TTCTGCT358.849576E-426.42857222
ATGCCCC2350.025.97872437
TTTGGAG601.3303033E-624.6666682
TTTTGGA601.3303033E-624.6666681
CTAAGGT551.8948153E-523.5454544
TCTTCTG400.001926912823.12500220
GAGAGCT400.001926912823.12500233
GCTTGAA400.001926912823.12500226
ATTGACT2250.023.02222331
AGGTAGC502.693773E-422.27
Produced by FastQC (version 0.11.5)