##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631488.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	246676
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74972838865556	34.0	31.0	34.0	31.0	34.0
2	32.864838897987646	34.0	33.0	34.0	31.0	34.0
3	32.87610468793073	34.0	33.0	34.0	31.0	34.0
4	36.32136081337463	37.0	37.0	37.0	35.0	37.0
5	36.329314566475865	37.0	37.0	37.0	35.0	37.0
6	36.40870615706433	37.0	37.0	37.0	35.0	37.0
7	36.399049765684545	37.0	37.0	37.0	35.0	37.0
8	36.41929089169599	37.0	37.0	37.0	35.0	37.0
9	38.223665861291735	39.0	39.0	39.0	37.0	39.0
10	38.19794386158362	39.0	39.0	39.0	37.0	39.0
11	38.25621057581605	39.0	39.0	39.0	37.0	39.0
12	38.074316106958115	39.0	39.0	39.0	37.0	39.0
13	38.1774230164264	39.0	39.0	39.0	37.0	39.0
14	39.54932786327004	41.0	39.0	41.0	37.0	41.0
15	39.52031004232272	41.0	39.0	41.0	37.0	41.0
16	39.42620279232678	40.0	39.0	41.0	37.0	41.0
17	39.42490959801521	40.0	39.0	41.0	37.0	41.0
18	39.593239715254015	41.0	39.0	41.0	37.0	41.0
19	39.6384731388542	41.0	40.0	41.0	37.0	41.0
20	39.66742609739091	41.0	40.0	41.0	37.0	41.0
21	39.65054970892993	41.0	40.0	41.0	37.0	41.0
22	39.57607955374662	41.0	40.0	41.0	37.0	41.0
23	39.50599166518024	41.0	39.0	41.0	36.0	41.0
24	39.45615301042663	41.0	39.0	41.0	36.0	41.0
25	39.41241142227051	41.0	39.0	41.0	36.0	41.0
26	39.245151534806794	41.0	39.0	41.0	35.0	41.0
27	39.08902365856427	41.0	39.0	41.0	35.0	41.0
28	38.90976827903809	40.0	38.0	41.0	35.0	41.0
29	38.70209100196209	40.0	38.0	41.0	35.0	41.0
30	38.470771376218195	40.0	37.0	41.0	35.0	41.0
31	38.18164718091748	40.0	37.0	41.0	35.0	41.0
32	37.937237509932054	40.0	35.0	41.0	35.0	41.0
33	37.71407433232256	40.0	35.0	41.0	34.0	41.0
34	37.55790997097407	40.0	35.0	41.0	34.0	41.0
35	37.352519093872125	40.0	35.0	41.0	33.0	41.0
36	37.14622825082294	40.0	35.0	41.0	33.0	41.0
37	36.927382477419776	39.0	35.0	41.0	33.0	41.0
38	36.66900711864957	39.0	35.0	41.0	33.0	41.0
39	36.340989800385934	39.0	35.0	41.0	31.0	41.0
40	36.051249412184404	39.0	35.0	41.0	31.0	41.0
41	35.660141237899104	39.0	35.0	41.0	28.0	41.0
42	35.27755030890723	39.0	35.0	41.0	24.0	41.0
43	34.224059089656066	37.0	34.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	2.0
13	2.0
14	0.0
15	3.0
16	1.0
17	7.0
18	2.0
19	11.0
20	23.0
21	52.0
22	101.0
23	166.0
24	300.0
25	472.0
26	653.0
27	1056.0
28	1639.0
29	2325.0
30	2924.0
31	3565.0
32	4281.0
33	5634.0
34	7735.0
35	10564.0
36	17029.0
37	40804.0
38	50921.0
39	96399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.915597788191796	14.027307074867437	16.69558449139762	24.36151064554314
2	19.885598923283982	20.853265011594154	33.97249833790073	25.28863772722113
3	19.968703886879958	23.110476900874023	29.818466328301092	27.10235288394493
4	13.517326371434594	19.282783894663442	31.04679822925619	36.153091504645765
5	13.900419984108709	39.56607047300913	29.092818109585046	17.44069143329712
6	26.930872885890807	30.900452415314017	22.316723151015907	19.851951547779272
7	30.424524477452202	32.19080899641635	17.134216543157827	20.250449982973617
8	35.81459079926705	28.318928472976694	16.549238677455445	19.317242050300802
9	32.375666866659095	13.121665666704502	16.622208889393374	37.880458577243026
10	21.758095639624447	27.19356564886734	21.82863351116444	29.21970520034377
11	33.112666007232164	27.29491316544779	14.759036144578314	24.833384682741734
12	25.455658434545718	30.86356191927873	23.31114498370332	20.36963466247223
13	35.33582513094099	18.79428886474566	25.116346949034362	20.75353905527899
14	24.104898733561434	21.87971265952099	25.409038576918714	28.606350029998868
15	32.744166436945626	24.373266957466473	20.326257925375796	22.5563086802121
16	23.712481149361917	26.334138708265094	26.04387942077867	23.909500721594316
17	18.22309426129822	31.760690136048908	26.707097569281164	23.30911803337171
18	22.737923429924273	22.76143605377094	29.910895263422464	24.589745252882324
19	21.855794645608004	30.418849016523698	30.466685044349674	17.258671293518624
20	24.893787802623685	22.85386498889231	32.53457977265725	19.71776743582675
21	28.309199111384974	21.15852373153448	29.9542720005189	20.578005156561645
22	26.244952893674295	22.38969336295383	26.520212748706808	24.845140994665066
23	22.323209392077057	25.445118292821352	26.79385104347403	25.43782127162756
24	21.14352429908058	25.0891858145908	32.19243055668164	21.574859329646987
25	20.357067570416255	25.049862978157584	28.740939532017705	25.852129919408455
26	25.100131346381488	23.44776143605377	27.971509186138903	23.480598031425835
27	22.611441729231867	23.702751787770193	27.1668099044901	26.518996578507842
28	20.444226434675443	24.513531920413822	30.630057241077363	24.41218440383337
29	19.994243461058232	25.81037474257731	32.70727594091035	21.48810585545412
30	19.77533282524445	26.387650197019575	34.61382542282184	19.223191554914138
31	19.899382185538926	28.774992297588742	29.473479381861228	21.852146135011107
32	18.89523099125979	27.987724788791773	33.81682855243315	19.300215667515282
33	21.029204300377824	25.179182409314244	33.40292529471858	20.388687995589354
34	21.768230391282493	26.94343997794678	27.76759798277903	23.520731647991695
35	20.238693671050285	28.280821806742445	28.79931570156805	22.68116882063922
36	17.085164345132885	27.700303231769606	31.130713972984807	24.083818450112698
37	18.44605879777522	27.4343673482625	31.47489013929203	22.644683714670254
38	19.16562616549644	23.294118600917805	33.668050398093044	23.87220483549271
39	19.863707859702608	23.28074072872918	34.523018047965756	22.332533363602458
40	18.878204608474274	22.837649386239438	31.72542120027891	26.558724805007376
41	15.033890609544503	24.680147237672088	35.26488186933467	25.021080283448732
42	15.298204932786327	23.57059462614928	34.03452301804796	27.096677423016423
43	13.946634451669397	26.861551184549775	35.96255817347452	23.229256190306312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	31.0
2	56.0
3	250.5
4	445.0
5	445.0
6	615.5
7	786.0
8	889.5
9	993.0
10	1615.5
11	2238.0
12	2238.0
13	3964.0
14	5690.0
15	8775.0
16	11860.0
17	10455.5
18	9051.0
19	9051.0
20	10105.5
21	11160.0
22	7772.5
23	4385.0
24	3553.5
25	2722.0
26	2722.0
27	2479.5
28	2237.0
29	2374.0
30	2511.0
31	2541.0
32	2571.0
33	2571.0
34	2474.5
35	2378.0
36	2343.5
37	2309.0
38	2896.0
39	3483.0
40	3483.0
41	4501.0
42	5519.0
43	7613.0
44	9707.0
45	13027.5
46	16348.0
47	16348.0
48	23742.0
49	31136.0
50	29540.5
51	27945.0
52	21942.0
53	15939.0
54	15939.0
55	12693.5
56	9448.0
57	9371.5
58	9295.0
59	9273.0
60	9251.0
61	9251.0
62	9275.5
63	9300.0
64	9140.5
65	8981.0
66	8585.5
67	8190.0
68	8190.0
69	7527.0
70	6864.0
71	6100.5
72	5337.0
73	4499.5
74	3662.0
75	3662.0
76	3014.0
77	2366.0
78	1867.5
79	1369.0
80	1024.5
81	680.0
82	680.0
83	486.0
84	292.0
85	193.5
86	95.0
87	71.0
88	47.0
89	47.0
90	32.0
91	17.0
92	10.0
93	3.0
94	3.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	246676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.23892499519909
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70212281629874	39.16535103836979
2	8.470134639774802	7.832998406155695
3	2.3237152400830383	3.2233828408920195
4	1.1020308849847267	2.038268937328066
5	0.6478872969295367	1.4978806064033563
6	0.4355765956240577	1.2084356120834983
7	0.29927774143630453	0.968679672730411
8	0.2316557511923791	0.8569210323272736
9	0.1908899973722516	0.7943893443746363
>10	1.310375197803133	11.77833840173548
>50	0.1491322075526124	4.995254467784801
>100	0.11334047773998542	10.524237118626676
>500	0.017895864906313484	6.086615972158178
>1k	0.003976858868069664	4.009627228788794
>5k	0.001988429434034832	5.0196193202413255
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5866	2.378018129043766	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5053	2.04843600512413	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4049	1.6414243785370284	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	1866	0.7564578637565065	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1695	0.6871361624154761	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	1112	0.45079375374985814	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	945	0.383093612674115	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	945	0.383093612674115	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	919	0.37255347094974783	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	918	0.37214808088342605	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	884	0.3583648186284843	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	837	0.33931148551135903	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	830	0.3364737550471063	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	817	0.33120368418492274	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	801	0.3247174431237737	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	689	0.2793137556957304	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.2659358835071105	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	650	0.2635035431091796	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	640	0.25944964244596147	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	576	0.23350467820136533	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	571	0.2314777278697563	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	536	0.21728907554849275	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	520	0.21080283448734372	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	506	0.20512737355883834	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	492	0.1994519126303329	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	484	0.19620879209975836	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	479	0.19418184176814932	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	444	0.1799931894468858	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	412	0.1670207073245877	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	404	0.1637775867940132	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	397	0.1609398563297605	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	380	0.15404822520228964	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	370	0.1499943245390715	No Hit
CTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATA	362	0.14675120400849698	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	342	0.13864340268206066	No Hit
GGGCATATCAATAAGCGGAGGAATCACCGACTGCCCATAGAGC	340	0.13783262254941706	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	339	0.1374272324830952	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCC	330	0.1337787218861989	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	306	0.12404936029447534	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	304	0.12323858016183172	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	303	0.1228331900955099	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	301	0.12202240996286627	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	298	0.12080623976390083	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	297	0.12040084969757901	No Hit
CATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGT	290	0.11756311923332631	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCTGT	283	0.1147253887690736	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCACTGTCT	281	0.11391460863642998	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGA	278	0.11269843843746453	ABI Solid3 Adapter B (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	276	0.1118876583048209	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	273	0.11067148810585546	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	264	0.10702297750895913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012161701989654445	0.0
2	0.0	0.0	0.0	0.0012161701989654445	0.0
3	0.0	0.0	0.0	0.0012161701989654445	0.0
4	0.0	0.0	0.0	0.0016215602652872594	0.0
5	0.0	0.0	0.0	0.0016215602652872594	0.0
6	0.0	0.0	0.0	0.0016215602652872594	0.0
7	0.0	0.0	0.0	0.0016215602652872594	0.0
8	0.0	0.0	0.0	0.0016215602652872594	0.0
9	0.0	0.0	0.0	0.0016215602652872594	0.0
10	0.0	0.0	0.0	0.0016215602652872594	0.0
11	0.0	0.0	0.0	0.0020269503316090744	0.0
12	0.0	0.0	0.0	0.002837730464252704	0.0
13	0.0	0.0	0.0	0.0032431205305745187	0.0
14	0.0	0.0	0.0	0.003648510596896334	0.0
15	0.0	0.0	0.0	0.005270070862183593	0.0
16	0.0	0.0	0.0	0.01013475165804537	0.0
17	0.0	0.0	0.0	0.013783262254941704	0.0
18	0.0	0.0	0.0	0.016215602652872595	0.0
19	0.0	0.0	0.0	0.017026382785516225	0.0
20	0.0	0.0	0.0	0.018647943050803483	4.0539006632181484E-4
21	0.0	0.0	0.0	0.022701843714021633	4.0539006632181484E-4
22	0.0	0.0	0.0	0.031215035106779743	4.0539006632181484E-4
23	0.0	0.0	0.0	0.04418751722907782	4.0539006632181484E-4
24	0.0	0.0	0.0	0.06932170134103034	4.0539006632181484E-4
25	0.0	0.0	0.0	0.07702411260114482	4.0539006632181484E-4
26	0.0	0.0	0.0	0.09688822585091375	4.0539006632181484E-4
27	0.0	0.0	0.0	0.1670207073245877	4.0539006632181484E-4
28	0.0	0.0	0.0	0.3936337543984822	4.0539006632181484E-4
29	0.0	0.0	0.0	0.753214743225932	4.0539006632181484E-4
30	0.0	0.0	0.0	1.2806272195106132	4.0539006632181484E-4
31	0.0	0.0	0.0	2.4059900436199713	4.0539006632181484E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAT	20	0.0018391348	37.0	1
TATTGCT	40	1.593331E-6	32.375	30
CAGAAAT	30	3.5913583E-4	30.833334	2
TATGGCA	30	3.5913583E-4	30.833334	4
AGAAATC	30	3.5913583E-4	30.833334	3
TTATTGC	45	3.9914994E-6	28.777777	29
ATATGCT	70	6.539267E-9	26.428572	35
TATGCTG	70	6.539267E-9	26.428572	36
TAACGAT	35	8.849576E-4	26.428572	23
GTCTTCT	35	8.849576E-4	26.428572	19
TTCTGCT	35	8.849576E-4	26.428572	22
ATGCCCC	235	0.0	25.978724	37
TTTGGAG	60	1.3303033E-6	24.666668	2
TTTTGGA	60	1.3303033E-6	24.666668	1
CTAAGGT	55	1.8948153E-5	23.545454	4
TCTTCTG	40	0.0019269128	23.125002	20
GAGAGCT	40	0.0019269128	23.125002	33
GCTTGAA	40	0.0019269128	23.125002	26
ATTGACT	225	0.0	23.022223	31
AGGTAGC	50	2.693773E-4	22.2	7
>>END_MODULE