FastQCFastQC Report
Fri 10 Feb 2017
ERR1631487.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631487.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420768
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.19773366795954064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6890.1637481937789946No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6060.14402235911476158No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC200.001840610537.021
GACTGTT303.595636E-430.8333327
TACGACC250.005492431629.64
GGTATCA3350.026.5074631
TAGGACA652.676139E-622.769234
TCTGTAC502.698158E-422.23
AATCTGC450.003821838620.55555517
AATGTAC450.003821838620.5555553
CGAACGA555.135648E-420.1818216
ACGAACG555.135648E-420.1818215
TATACAC2100.018.50000237
AGCGTCA500.007027650718.53
AGTCATC500.007027650718.58
TAACGAA609.223558E-418.513
GCATTAG500.007027650718.51
ACGCTAT500.007027650718.527
ATAACGA609.223558E-418.512
TAGGAGT609.223558E-418.54
GTATCAA4800.017.7291662
GACGGTA1054.779413E-717.619058
Produced by FastQC (version 0.11.5)