FastQCFastQC Report
Fri 10 Feb 2017
ERR1631486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70718
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29424.160185525608756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19552.7645012585197546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19372.739048050001414No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7841.1086286376877175No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4330.6122910715800787No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3520.4977516332475466No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2440.3450323821375039No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA2310.32664950931870246No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.3139229050595322No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.2955400322407308No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA2030.287055629401284No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.28422749512146833No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1990.28139936084165273No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT1940.27432902514211377No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1860.2630164880228513No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1850.26160242088294355No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1850.26160242088294355No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.25877428660312796No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.24180548092423426No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1670.23614921236460307No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1650.23332107808478747No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1510.21352413812607823No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT1510.21352413812607823No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT1510.21352413812607823No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT1490.2106960038462626No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1360.19231313102746117No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.18665686246782998No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.18100059390819878No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC1190.16827398964902854No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT1140.16120365394948952No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA1110.1569614525297661No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA1080.1527192511100427No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.1513051839701349No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG1050.1484770496903193No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT1030.1456489154105037No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA1020.1442348482705959No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT990.13999264685087248No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT930.13150824401142566No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT920.13009417687151786No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.13009417687151786No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT910.12868010973161007No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT880.12443790831188666No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC880.12443790831188666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC860.12160977403207106No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.11595350547243984No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA820.11595350547243984No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT800.11312537119262421No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT800.11312537119262421No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT800.11312537119262421No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC790.11171130405271641No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA770.10888316977290081No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG770.10888316977290081No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT760.10746910263299302No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC750.1060550354930852No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT740.1046409683531774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC730.1032269012132696No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA720.10181283407336181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG710.10039876693345401No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG200.001830278337.018
TGGGTGG200.001830278337.019
ATGCCCC554.838512E-1033.63636437
TGAACCA250.00546195529.5999985
GGGCAGT3100.024.4677437
TGGGCAG3100.024.4677436
GGCAGTC3150.024.0793658
CAGTCGG3200.023.70312510
GCAGTCG3200.023.7031259
AGTCGGT3150.023.49206411
ATTCCTC3250.022.7692319
CGGTGAT3350.021.53731314
GATTCCT3350.021.53731318
GTCGGTG3350.021.53731312
TTCCTCG2500.021.4620
TCCTCGC2550.021.03921521
TGATTCC3350.020.98507517
TCGGTGA3450.020.91304213
GTGATTC3400.020.67647216
GGTGATT3500.020.61428515
Produced by FastQC (version 0.11.5)