##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631486.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70718
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71493820526599	34.0	31.0	34.0	31.0	34.0
2	32.80454763992194	34.0	31.0	34.0	31.0	34.0
3	32.815223846828246	34.0	31.0	34.0	31.0	34.0
4	36.26450125851976	37.0	37.0	37.0	35.0	37.0
5	36.30859187194208	37.0	37.0	37.0	35.0	37.0
6	36.41657003874544	37.0	37.0	37.0	35.0	37.0
7	36.410517831386635	37.0	37.0	37.0	35.0	37.0
8	36.44519075765717	37.0	37.0	37.0	35.0	37.0
9	38.22807488899573	39.0	39.0	39.0	37.0	39.0
10	38.21486750190899	39.0	39.0	39.0	37.0	39.0
11	38.28391640035069	39.0	39.0	39.0	37.0	39.0
12	38.081026047116715	39.0	39.0	39.0	37.0	39.0
13	38.208560762465005	39.0	39.0	39.0	37.0	39.0
14	39.48361096184847	41.0	39.0	41.0	37.0	41.0
15	39.444724115501	41.0	39.0	41.0	37.0	41.0
16	39.369538165672104	40.0	39.0	41.0	37.0	41.0
17	39.468975366950424	40.0	39.0	41.0	37.0	41.0
18	39.661132950592496	41.0	39.0	41.0	38.0	41.0
19	39.710483893775276	41.0	40.0	41.0	37.0	41.0
20	39.72076416188241	41.0	40.0	41.0	38.0	41.0
21	39.68832546169292	41.0	40.0	41.0	37.0	41.0
22	39.594869764416416	41.0	40.0	41.0	36.0	41.0
23	39.47738906643288	41.0	39.0	41.0	35.0	41.0
24	39.41047540937244	41.0	39.0	41.0	35.0	41.0
25	39.35959727367855	41.0	39.0	41.0	35.0	41.0
26	39.14194405950394	41.0	39.0	41.0	35.0	41.0
27	38.93725784100229	41.0	38.0	41.0	35.0	41.0
28	38.74215899770921	40.0	38.0	41.0	35.0	41.0
29	38.45523063435052	40.0	37.0	41.0	35.0	41.0
30	38.116123193529226	40.0	36.0	41.0	35.0	41.0
31	37.697036115274756	39.0	35.0	41.0	35.0	41.0
32	37.37617014055827	39.0	35.0	41.0	35.0	41.0
33	37.07528493452869	39.0	35.0	41.0	34.0	41.0
34	36.84153963630193	39.0	35.0	41.0	33.0	41.0
35	36.54259170225402	39.0	35.0	41.0	33.0	41.0
36	36.25328770610029	39.0	35.0	41.0	33.0	41.0
37	35.91734777567239	38.0	35.0	41.0	31.0	41.0
38	35.60013009417687	38.0	35.0	41.0	30.0	41.0
39	35.16292881586018	38.0	35.0	41.0	24.0	41.0
40	34.72483667524534	38.0	35.0	41.0	21.0	41.0
41	34.221513617466556	37.0	35.0	41.0	15.0	41.0
42	33.69699369326056	37.0	35.0	41.0	10.0	41.0
43	32.64857603439011	35.0	33.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	3.0
18	3.0
19	8.0
20	16.0
21	25.0
22	37.0
23	68.0
24	120.0
25	180.0
26	264.0
27	421.0
28	686.0
29	911.0
30	1151.0
31	1306.0
32	1535.0
33	1862.0
34	2550.0
35	3363.0
36	5058.0
37	13618.0
38	13124.0
39	24404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.47164795384485	14.8788144461099	18.692553522441248	18.956984077604005
2	18.121270397918494	24.956870952232812	34.08608840747759	22.835770242371108
3	19.7714867501909	26.96767442518171	31.706213411012758	21.55462541361464
4	11.98987527927826	20.5506377442801	30.16063802709353	37.29884894934811
5	10.888316977290081	42.97208631465822	28.820102378460927	17.319494329590768
6	24.104188466868408	34.216182584349106	21.157272547300547	20.522356401481943
7	25.535224412455104	35.54681976300235	18.255606776209735	20.662349048332814
8	35.36864730337396	28.69707853728895	17.593823354732884	18.340450804604203
9	31.197149240645945	12.631861760796404	18.249950507650105	37.92103849090755
10	20.841935575101108	31.291891739019768	21.157272547300547	26.708900138578578
11	30.37699029949942	28.139936084165274	15.632512231680758	25.85056138465454
12	23.368873554116348	36.35142396560988	23.90904720156113	16.370655278712633
13	34.24022172572754	20.5690206170989	27.0935264006335	18.097231256540063
14	23.828445374586384	23.414123702593397	29.692581803784044	23.06484911903617
15	28.73667241720637	27.464011991289343	24.700924799909497	19.098390791594785
16	18.61902203116604	28.858282191238438	29.28250233321078	23.24019344438474
17	14.32591419440595	34.59656664498431	29.170791029158067	21.90672813145168
18	17.725331598744308	25.003535167849773	35.47187420458723	21.79925902881869
19	18.620436098305948	30.58910037048559	36.92553522441246	13.864928306796006
20	22.093384993919514	24.41669730478803	38.251930201645976	15.237987499646483
21	26.05277298566136	23.039395910517833	34.82140332022964	16.086427783591166
22	22.91778613648576	25.187363896037784	31.78822930512741	20.106620662349048
23	18.944257473344834	28.079131197149245	32.47263780084278	20.503973528663142
24	16.743969003648292	28.210639441160666	37.563279504510874	17.482112050680165
25	16.463983709946547	27.851466387624086	34.63333239062191	21.05121751180746
26	20.8051698294635	25.894397465991688	34.18224497299131	19.118187731553494
27	18.742045872338018	27.21513617466557	32.91099861421421	21.131819338782208
28	16.476710314205718	27.219378376085295	37.88144461099013	18.422466698718857
29	17.05082157300829	28.364772759410616	37.90972595378829	16.674679713792813
30	16.582765349698803	28.712633275827937	39.21490992392319	15.489691450550072
31	16.57710908113917	30.74606182301536	35.722164088350915	16.954665007494558
32	15.698973387256427	29.807121242116573	39.241777199581435	15.252128171045563
33	16.639328035295115	28.73667241720637	38.843010266127436	15.78098928137108
34	17.014055827370683	28.988376368109957	33.971548969145054	20.026018835374305
35	14.990525750162616	30.535365819169098	34.58525410786504	19.888854322803244
36	13.05891003704856	29.542690686953815	36.18173590882095	21.216663367176675
37	13.642919765830483	29.781668033598237	37.03159025990554	19.543821940665744
38	14.450352102717837	26.516587007551117	38.70867388783619	20.32438700189485
39	15.028705562940129	26.905455471025764	39.41287932351028	18.65295964252383
40	14.188749681834894	26.98605729800051	37.06694193840324	21.75825108176136
41	11.30122458214316	28.94595435391272	38.87836194462513	20.874459119318985
42	11.315365253542238	28.076303062869425	37.60994372012783	22.998387963460505
43	10.198252213015074	31.27633700048078	38.138804830453346	20.386605956050794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	23.0
2	41.0
3	156.0
4	271.0
5	271.0
6	390.0
7	509.0
8	569.0
9	629.0
10	926.0
11	1223.0
12	1223.0
13	1987.5
14	2752.0
15	4205.0
16	5658.0
17	4863.5
18	4069.0
19	4069.0
20	4278.0
21	4487.0
22	3177.5
23	1868.0
24	1482.5
25	1097.0
26	1097.0
27	984.5
28	872.0
29	921.0
30	970.0
31	940.5
32	911.0
33	911.0
34	906.5
35	902.0
36	889.5
37	877.0
38	1109.0
39	1341.0
40	1341.0
41	1771.0
42	2201.0
43	2921.5
44	3642.0
45	4596.0
46	5550.0
47	5550.0
48	6757.0
49	7964.0
50	7117.5
51	6271.0
52	4788.5
53	3306.0
54	3306.0
55	2648.0
56	1990.0
57	1851.0
58	1712.0
59	1694.5
60	1677.0
61	1677.0
62	1649.0
63	1621.0
64	1594.5
65	1568.0
66	1479.5
67	1391.0
68	1391.0
69	1261.0
70	1131.0
71	998.5
72	866.0
73	734.5
74	603.0
75	603.0
76	488.5
77	374.0
78	289.5
79	205.0
80	154.0
81	103.0
82	103.0
83	71.0
84	39.0
85	26.5
86	14.0
87	10.0
88	6.0
89	6.0
90	3.0
91	0.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	70718.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.870556294012836
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.79052662547808	46.41533980033372
2	5.870710636818315	6.207754744195254
3	1.979191740886357	3.139229050595322
4	1.072507956885715	2.2681636924121156
5	0.6472491909385114	1.7110212392884416
6	0.47875043461980793	1.5187081082609803
7	0.32095001203562545	1.1878163975225544
8	0.22199042499130758	0.9389405808987811
9	0.18722084035411485	0.8908622981419158
>10	1.2222846291690068	12.780338810486722
>50	0.1123325042124689	4.254928023982578
>100	0.08558666987616678	7.914533782063972
>500	0.002674583433630212	1.1086286376877175
>1k	0.008023750300890637	9.663734834129926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2942	4.160185525608756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1955	2.7645012585197546	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1937	2.739048050001414	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	784	1.1086286376877175	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	433	0.6122910715800787	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	352	0.4977516332475466	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	244	0.3450323821375039	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	231	0.32664950931870246	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.3139229050595322	No Hit
GCCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	209	0.2955400322407308	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	203	0.287055629401284	No Hit
CCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	201	0.28422749512146833	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	199	0.28139936084165273	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	194	0.27432902514211377	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	186	0.2630164880228513	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	185	0.26160242088294355	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	185	0.26160242088294355	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.25877428660312796	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.24180548092423426	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	167	0.23614921236460307	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	165	0.23332107808478747	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	151	0.21352413812607823	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCTCT	151	0.21352413812607823	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	151	0.21352413812607823	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	149	0.2106960038462626	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	136	0.19231313102746117	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.18665686246782998	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	128	0.18100059390819878	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	119	0.16827398964902854	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCT	114	0.16120365394948952	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACTGTCTCTTA	111	0.1569614525297661	No Hit
CTCTATGGGCATATCAATAAGCGGAGGAATCACCGACTGCCCA	108	0.1527192511100427	No Hit
GCCCATAGAGAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.1513051839701349	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	105	0.1484770496903193	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	103	0.1456489154105037	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTTA	102	0.1442348482705959	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	99	0.13999264685087248	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	93	0.13150824401142566	No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	92	0.13009417687151786	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.13009417687151786	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCT	91	0.12868010973161007	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCTGTCTCTT	88	0.12443790831188666	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	88	0.12443790831188666	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	86	0.12160977403207106	No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.11595350547243984	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA	82	0.11595350547243984	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACCTGTCT	80	0.11312537119262421	No Hit
CCCATAGAGAGGCCCATGTACTCTGCGTTGATACCTGTCTCTT	80	0.11312537119262421	No Hit
TTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTGAT	80	0.11312537119262421	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	79	0.11171130405271641	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	77	0.10888316977290081	No Hit
CCTTATTGATATGCCCATAGAGAGGCCCATGTACTCTGCGTTG	77	0.10888316977290081	No Hit
TGCCCATAGAGAGGCCCATGTACTCTGCGTTGATACCCTGTCT	76	0.10746910263299302	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	75	0.1060550354930852	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	74	0.1046409683531774	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	73	0.1032269012132696	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	72	0.10181283407336181	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	71	0.10039876693345401	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.008484402839446816	0.0
4	0.0	0.0	0.0	0.008484402839446816	0.0
5	0.0	0.0	0.0	0.008484402839446816	0.0
6	0.0	0.0	0.0	0.008484402839446816	0.0
7	0.0	0.0	0.0	0.008484402839446816	0.0
8	0.0	0.0	0.0	0.011312537119262422	0.0
9	0.0	0.0	0.0	0.014140671399078028	0.0
10	0.0	0.0	0.0	0.016968805678893632	0.0
11	0.0	0.0	0.0	0.028281342798156056	0.0
12	0.0	0.0	0.0	0.029695409938063858	0.0
13	0.0	0.0	0.0	0.029695409938063858	0.0
14	0.0	0.0	0.0	0.032523544217879466	0.0
15	0.0	0.0	0.0	0.03535167849769507	0.0
16	0.0	0.0	0.0	0.04383608133714189	0.0
17	0.0	0.0	0.0	0.0494923498967731	0.0
18	0.0	0.0	0.0	0.050906417036680904	0.0
19	0.0	0.0	0.0	0.05514861845640431	0.0
20	0.0	0.0	0.0	0.05797675273621992	0.0
21	0.0	0.0	0.0	0.07070335699539014	0.0
22	0.0	0.0	0.0	0.10181283407336181	0.0
23	0.0	0.0	0.0	0.13857857971096468	0.0
24	0.0	0.0	0.0	0.200797533866908	0.0
25	0.0	0.0	0.0	0.23049294380497187	0.0
26	0.0	0.0	0.0	0.27008682372239035	0.0
27	0.0	0.0	0.0	0.36624338923612093	0.0
28	0.0	0.0	0.0	0.716932039933256	0.0
29	0.0	0.0	0.0	1.4522469526853135	0.0
30	0.0	0.0	0.0	2.7857122656183715	0.0
31	0.0	0.0	0.0	4.676320031675104	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTG	20	0.0018302783	37.0	18
TGGGTGG	20	0.0018302783	37.0	19
ATGCCCC	55	4.838512E-10	33.636364	37
TGAACCA	25	0.005461955	29.599998	5
GGGCAGT	310	0.0	24.467743	7
TGGGCAG	310	0.0	24.467743	6
GGCAGTC	315	0.0	24.079365	8
CAGTCGG	320	0.0	23.703125	10
GCAGTCG	320	0.0	23.703125	9
AGTCGGT	315	0.0	23.492064	11
ATTCCTC	325	0.0	22.76923	19
CGGTGAT	335	0.0	21.537313	14
GATTCCT	335	0.0	21.537313	18
GTCGGTG	335	0.0	21.537313	12
TTCCTCG	250	0.0	21.46	20
TCCTCGC	255	0.0	21.039215	21
TGATTCC	335	0.0	20.985075	17
TCGGTGA	345	0.0	20.913042	13
GTGATTC	340	0.0	20.676472	16
GGTGATT	350	0.0	20.614285	15
>>END_MODULE