FastQCFastQC Report
Fri 10 Feb 2017
ERR1631485.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631485.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241037
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.3202827781626887No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6350.2634450312607608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5300.2198832544381153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.10537801250430433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT251.2307325E-437.04
AATTCCG250.00548783429.65
TAATACT358.8489865E-426.428574
GTCTGGA358.8489865E-426.428571
GACCATA509.052219E-625.90000211
CGACCAT551.8945817E-523.54545410
ATACACA1103.6379788E-1223.54545437
ATTCCGA400.001926785923.1256
TACTGTT400.001926785923.1257
GGTATCA3350.022.641791
CGTGGGT603.7105798E-521.58333233
TAAACGA603.7105798E-521.58333216
CTCGATT603.7105798E-521.58333225
ATCGGCC450.003817170920.55555728
ATTCCGT656.873245E-519.92307729
AAACGAT656.873245E-519.92307717
GATAGCT656.873245E-519.92307714
TTCCGAC759.216044E-619.7333327
TCCGACC801.608049E-518.58
ATAGCTC701.2141313E-418.515
Produced by FastQC (version 0.11.5)