##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631485.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241037
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77113887079577	34.0	31.0	34.0	31.0	34.0
2	32.9463650808797	34.0	33.0	34.0	31.0	34.0
3	33.06818870131971	34.0	33.0	34.0	31.0	34.0
4	36.40759302513722	37.0	37.0	37.0	35.0	37.0
5	36.37447777727071	37.0	37.0	37.0	35.0	37.0
6	36.41517692304501	37.0	37.0	37.0	35.0	37.0
7	36.41228110207147	37.0	37.0	37.0	35.0	37.0
8	36.40460178312873	37.0	37.0	37.0	35.0	37.0
9	38.25049266295216	39.0	39.0	39.0	37.0	39.0
10	38.20622560021906	39.0	39.0	39.0	37.0	39.0
11	38.27628538357182	39.0	39.0	39.0	37.0	39.0
12	38.04653227512789	39.0	39.0	39.0	37.0	39.0
13	38.177321324112064	39.0	39.0	39.0	37.0	39.0
14	39.68329343627742	41.0	40.0	41.0	37.0	41.0
15	39.67807846928065	41.0	40.0	41.0	37.0	41.0
16	39.64717450018047	41.0	40.0	41.0	37.0	41.0
17	39.643955077436246	41.0	40.0	41.0	37.0	41.0
18	39.63305218700863	41.0	40.0	41.0	37.0	41.0
19	39.641021917796856	41.0	40.0	41.0	37.0	41.0
20	39.596593054178406	41.0	40.0	41.0	37.0	41.0
21	39.564946460501915	41.0	40.0	41.0	37.0	41.0
22	39.51310379734232	41.0	39.0	41.0	37.0	41.0
23	39.452349639267	41.0	39.0	41.0	37.0	41.0
24	39.403651721519935	41.0	39.0	41.0	36.0	41.0
25	39.395486999921175	41.0	39.0	41.0	36.0	41.0
26	39.300161386011276	41.0	39.0	41.0	36.0	41.0
27	39.23992996925783	41.0	39.0	41.0	36.0	41.0
28	39.167102975891666	41.0	39.0	41.0	35.0	41.0
29	39.09669469832432	41.0	39.0	41.0	35.0	41.0
30	39.037712052506464	40.0	39.0	41.0	35.0	41.0
31	38.96647402680916	40.0	39.0	41.0	35.0	41.0
32	38.882731696793435	40.0	38.0	41.0	35.0	41.0
33	38.79477839501819	40.0	38.0	41.0	35.0	41.0
34	38.74714255487747	40.0	38.0	41.0	35.0	41.0
35	38.652522226878034	40.0	38.0	41.0	35.0	41.0
36	38.5966843264727	40.0	38.0	41.0	35.0	41.0
37	38.509195683650226	40.0	38.0	41.0	35.0	41.0
38	38.422623912511355	40.0	38.0	41.0	35.0	41.0
39	38.33263357907707	40.0	38.0	41.0	34.0	41.0
40	38.22837572654821	40.0	38.0	41.0	34.0	41.0
41	38.13082638765003	40.0	37.0	41.0	34.0	41.0
42	38.020432547700146	40.0	37.0	41.0	34.0	41.0
43	37.103150138775376	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	0.0
15	2.0
16	0.0
17	1.0
18	7.0
19	10.0
20	23.0
21	32.0
22	70.0
23	117.0
24	183.0
25	303.0
26	446.0
27	637.0
28	854.0
29	1227.0
30	1696.0
31	2081.0
32	2789.0
33	3721.0
34	5444.0
35	7785.0
36	12384.0
37	23104.0
38	51757.0
39	126362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.741417292780774	17.627999020897207	12.067441928002754	26.563141758319265
2	19.866659475516208	19.672913287171678	32.832303754195415	27.628123483116703
3	20.487725950787638	21.32079307326261	28.544580292652167	29.646900683297584
4	15.328352078726503	15.418379750826636	32.543966279036	36.70930189141086
5	16.696191870957573	34.25200280454868	31.990109402290933	17.061695922202816
6	35.42568153437024	32.95593622555873	15.63701838306982	15.981363857001208
7	30.86497093807175	29.05570514070454	19.995270435659258	20.084053485564457
8	29.048237407534945	30.964955587731346	19.201201475292176	20.78560552944154
9	27.579168343449346	13.933545472271891	18.983392591178948	39.50389359309982
10	19.07383513734406	25.02769284383725	30.49282890178686	25.405643117031822
11	37.89791608757162	20.657824317428446	19.906487385754055	21.537772209245883
12	23.649066325916767	24.617797267639407	26.718719532685853	25.014416873757973
13	31.956919477092725	18.22500280039994	23.45407551537731	26.364002207130028
14	24.163095292423982	19.659637317092397	23.29642337068583	32.88084401979779
15	27.27340615756087	25.725925895194514	20.778967544401898	26.221700402842718
16	26.679721370577962	24.297099615411742	22.84130652140543	26.181872492604867
17	25.232225757871195	25.104444545858108	23.770624426955198	25.892705269315496
18	25.52388222555043	23.326294303364214	25.0156614959529	26.134161975132447
19	26.119226508793258	24.12783099690089	24.82274505573833	24.93019743856752
20	26.060729265631416	23.39931213880027	24.79162950086501	25.748329094703305
21	27.371731310960556	23.4893398109004	23.80091023369856	25.33801864444048
22	27.41612283591316	23.226724527769598	23.748636101511387	25.608516534805858
23	26.38930952509366	23.262818571422645	24.074312242518783	26.273559660964914
24	25.81014533038496	23.736604753627034	24.099204686417437	26.354045229570566
25	26.197637707074016	23.166567788347848	24.12783099690089	26.507963507677246
26	25.729244887714337	23.939063297336094	24.26847330492829	26.063218510021287
27	26.100557175869266	23.42586407895883	24.16765890713874	26.305919838033166
28	25.46455523425864	23.80132510776354	24.75097184249721	25.983147815480613
29	25.256288453639897	23.797176367113764	25.128507241626803	25.818027937619537
30	25.04885142115111	24.185083617867797	24.958408874986	25.807656085995095
31	25.683193866501824	24.057302405854703	24.166414284943805	26.093089442699668
32	25.007364014653348	23.663172044126004	24.813202952243845	26.516260988976796
33	24.77130067168111	23.568580757311118	25.295701489812767	26.364417081195
34	25.720532532349804	22.993980177317177	25.22558777283156	26.05989951750146
35	25.227662143156444	23.165738040217892	25.794380115915814	25.81221970070985
36	24.563863639192324	23.213033683625337	25.69439546625622	26.52870721092612
37	24.635221978368467	22.241398623447854	26.40051112484805	26.72286827333563
38	23.87475781726457	21.861788853993367	27.67127038587437	26.592182942867694
39	24.03033559163116	21.5004335433979	27.96209710542365	26.50713375954729
40	23.39184440563067	21.558930786559742	28.64788393483158	26.401340872978007
41	22.579106112339602	21.37597132390463	29.487174168281214	26.557748395474555
42	22.55006492779117	21.77093143376328	29.489248538606105	26.189755099839445
43	21.687541746702788	21.17185328393566	29.727386251903237	27.413218717458314
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	49.0
2	42.0
3	95.5
4	149.0
5	149.0
6	169.5
7	190.0
8	184.5
9	179.0
10	281.0
11	383.0
12	383.0
13	587.5
14	792.0
15	1197.0
16	1602.0
17	1468.0
18	1334.0
19	1334.0
20	1555.5
21	1777.0
22	1560.5
23	1344.0
24	1505.0
25	1666.0
26	1666.0
27	1936.5
28	2207.0
29	2746.0
30	3285.0
31	3798.5
32	4312.0
33	4312.0
34	5204.5
35	6097.0
36	7121.0
37	8145.0
38	9096.5
39	10048.0
40	10048.0
41	10856.5
42	11665.0
43	12479.0
44	13293.0
45	14221.0
46	15149.0
47	15149.0
48	16049.5
49	16950.0
50	17213.5
51	17477.0
52	17734.5
53	17992.0
54	17992.0
55	17750.0
56	17508.0
57	17089.5
58	16671.0
59	16350.5
60	16030.0
61	16030.0
62	14926.0
63	13822.0
64	12604.5
65	11387.0
66	10173.5
67	8960.0
68	8960.0
69	7831.5
70	6703.0
71	5834.5
72	4966.0
73	4084.0
74	3202.0
75	3202.0
76	2602.0
77	2002.0
78	1686.0
79	1370.0
80	1150.0
81	930.0
82	930.0
83	752.5
84	575.0
85	493.5
86	412.0
87	320.0
88	228.0
89	228.0
90	164.5
91	101.0
92	61.5
93	22.0
94	15.0
95	8.0
96	8.0
97	5.0
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	241037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.32968407022517
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.330401207459	72.322111566574
2	4.3882292885489305	6.874572275994933
3	1.1795373263043414	2.771783583553715
4	0.5997674578540823	1.8791838195724941
5	0.3195632883576279	1.251564570874763
6	0.22149558337824776	1.0409807441381018
7	0.1690345007835234	0.9268293330339133
8	0.1199170636842439	0.7514452570412719
9	0.08405664520032896	0.5925717416290299
>10	0.5386082296950675	7.855495320078441
>50	0.04195444397889429	2.278763640331587
>100	0.005841758022377686	0.6489359764361584
>500	0.0015932067333757328	0.8057621707415634
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	772	0.3202827781626887	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.2634450312607608	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.2198832544381153	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	254	0.10537801250430433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	4.148740649775761E-4	0.0	0.0
13	0.0	0.0	4.148740649775761E-4	0.0	0.0
14	0.0	0.0	4.148740649775761E-4	0.0	0.0
15	0.0	0.0	4.148740649775761E-4	4.148740649775761E-4	0.0
16	0.0	0.0	4.148740649775761E-4	8.297481299551522E-4	0.0
17	0.0	0.0	4.148740649775761E-4	0.001244622194932728	0.0
18	0.0	0.0	4.148740649775761E-4	0.0016594962599103043	0.0
19	0.0	0.0	4.148740649775761E-4	0.0016594962599103043	0.0
20	0.0	0.0	4.148740649775761E-4	0.0016594962599103043	0.0
21	0.0	0.0	4.148740649775761E-4	0.0016594962599103043	0.0
22	0.0	0.0	4.148740649775761E-4	0.0016594962599103043	0.0
23	0.0	0.0	4.148740649775761E-4	0.002489244389865456	0.0
24	0.0	0.0	4.148740649775761E-4	0.007052859104618793	0.0
25	0.0	0.0	4.148740649775761E-4	0.012031347884349706	0.0
26	0.0	0.0	4.148740649775761E-4	0.018254458859013348	0.0
27	0.0	0.0	4.148740649775761E-4	0.07841119828076187	0.0
28	0.0	0.0	4.148740649775761E-4	0.264274779390716	0.0
29	0.0	0.0	4.148740649775761E-4	0.4974340039081137	0.0
30	0.0	0.0	4.148740649775761E-4	0.8019515676016545	0.0
31	0.0	0.0	4.148740649775761E-4	1.4740475528653276	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	25	1.2307325E-4	37.0	4
AATTCCG	25	0.005487834	29.6	5
TAATACT	35	8.8489865E-4	26.42857	4
GTCTGGA	35	8.8489865E-4	26.42857	1
GACCATA	50	9.052219E-6	25.900002	11
CGACCAT	55	1.8945817E-5	23.545454	10
ATACACA	110	3.6379788E-12	23.545454	37
ATTCCGA	40	0.0019267859	23.125	6
TACTGTT	40	0.0019267859	23.125	7
GGTATCA	335	0.0	22.64179	1
CGTGGGT	60	3.7105798E-5	21.583332	33
TAAACGA	60	3.7105798E-5	21.583332	16
CTCGATT	60	3.7105798E-5	21.583332	25
ATCGGCC	45	0.0038171709	20.555557	28
ATTCCGT	65	6.873245E-5	19.923077	29
AAACGAT	65	6.873245E-5	19.923077	17
GATAGCT	65	6.873245E-5	19.923077	14
TTCCGAC	75	9.216044E-6	19.733332	7
TCCGACC	80	1.608049E-5	18.5	8
ATAGCTC	70	1.2141313E-4	18.5	15
>>END_MODULE